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Biopesticides containing beneficial fungi are often grown on grains or other solids, but U.S. Department of Agriculture (USDA) scientists have shown a liquid diet can work better.
The approach, dubbed "liquid culture fermentation," offers several benefits, including lower material costs and increased yields of certain forms of insect-killing fungi, including Isaria or Metarhizium, which can serve as biobased alternatives to synthetic pesticides.
For decades, biopesticide makers have grown such fungi on moistened grains or other solid materials to prompt them to churn out billions of specialized cells called "conidia," which penetrate the bodies of silverleaf whiteflies, aphids and other soft-bodied insect pests, killing the pests within a few days.
Over the past several years, however, scientists with USDA's Agricultural Research Service (ARS) have sought to improve on the practice by initiating liquid culture fermentation processes in special tanks called bioreactors. ARS is USDA's chief intramural scientific research agency.
According to Mark Jackson, a microbiologist with the ARS Crop Bioprotection Research Unit in Peoria, Ill., using liquid culture fermentation has significantly reduced production costs, especially those associated with nitrogen as a primary fungal nutrient. One nitrogen source, called hydrolyzed protein, is typically derived from agricultural commodities like milk casein, which can sell for more than $6 a pound. Liquid culture fermentation's use of less expensive nitrogen sources, including soybean flour or cottonseed meal, reduces the cost to 30 to 50 cents a pound.
Conidia have long been the spores of choice for biopesticide uses. But the researchers showed that other fungal cells can be just as effective, including "blastospores"
|Contact: Jan Suszkiw|
United States Department of Agriculture - Research, Education and Economics