Navigation Links
Nature: Microscope looks into cells of living fish
Date:5/16/2012

This release is available in German.

Microscopes provide valuable insights in the structure and dynamics of cells, in particular when the latter remain in their natural environment. However, this is very difficult especially for higher organisms. Researchers of Karlsruhe Institute of Technology (KIT), the Max Planck Institute for Polymer Research, Mainz, and the American National Institutes of Health (NIH) have now developed a new method to visualize cell structures of an eighth of a micrometer in size in living fish larvae. It is published in the Nature Methods magazine (DOI:10.1038/nmeth.2025). "The zebrafish is perfectly suited for genetic studies of cells, as its larvae are completely transparent," explains Marina Mione, KIT. To visualize certain structures, these are colored mostly by genetic engineering methods using a fluorescent dye. Mione studied parts of the cellular skeleton of fish, the so-called microtubuli. The thread-shaped microtubuli have a length of about 100 m and a diameter of about 20 nm, corresponding to a hundred thousandth of a human hair. "Microtubuli exist everywhere in the cell and are required for its division and motion."

In the new microscopy method, the object is not illuminated completely, but only at a certain spot with special light. Scattered light is minimized and the illuminated detail is represented sharply. A series of images taken at variable illumination is then processed by a computer. In this way, an overall image is obtained. Smart illumination even allows to adjust the depth of field, to image various depth levels, and to combine them into a three-dimensional image on the computer. "Meanwhile, it is possible to reach resolutions of 145 nm in the plane and 400 nm in-between," says Marina Mione. The images are taken within a few seconds, such that movement of the cells does not cause any unsharpness.

Based on a series of images, videos of the movement of the microtubuli are obtained. In the experiment, it was observed over a period of 60 minutes how the early stage of the fish's lateral line develops about 45 m below the skin of the fish. Via this organ, the fish perceives movement stimuli in water. Such images of living organisms also provide valuable findings regarding the development of vertebrates on the cellular level.

The tropical zebrafish living in freshwater has several advantages as a genetic model organism. It is sufficiently small for easy cultivation and large enough to easily distinguish individual organs. It has a short generation cycle and produces many offsprings. As a vertebrate, it has a number of microbiological properties in common with human beings.


'/>"/>

Contact: Monika Landgraf
presse@kit.edu
49-721-608-47414
Helmholtz Association of German Research Centres
Source:Eurekalert  

Related biology news :

1. Changing the course of nature: Are fisheries directing the evolution of fish populations?
2. Commentary in Nature: Can economy bear what oil prices have in store?
3. Nature: Video reveals wave character of particles
4. Microscope reveals how bacteria breathe toxic metals
5. Computational microscope peers into the working ribosome
6. Compact microscope a marvel
7. New microscope reveals ultrastructure of cells
8. International conference puts food safety under the microscope
9. New microscope decodes complex eye circuitry
10. Brandeis researchers use lasers, custom microscope to show gene splicing process in real time
11. Big size multitouch display turned into a microscope
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Nature: Microscope looks into cells of living fish
(Date:4/18/2017)...  Socionext Inc., a global expert in SoC-based imaging and computing ... M820, which features the company,s hybrid codec technology. A demonstration utilizing ... Inc., will be showcased during the upcoming Medtec Japan at Tokyo ... Las Vegas Convention Center April 24-27. ... Click here for an image ...
(Date:4/11/2017)... Apr. 11, 2017 Research and Markets has ... report to their offering. ... The global eye tracking market to grow at a CAGR of ... Eye Tracking Market 2017-2021, has been prepared based on an in-depth ... market landscape and its growth prospects over the coming years. The ...
(Date:4/5/2017)... -- Today HYPR Corp. , leading innovator in ... the HYPR platform is officially FIDO® Certified . ... that empowers biometric authentication across Fortune 500 enterprises and ... 15 million users across the financial services industry, however ... suites and physical access represent a growing portion of ...
Breaking Biology News(10 mins):
(Date:4/21/2017)... ... April 21, 2017 , ... The 2017 Colorado ... with over 30 nominees and well as the first-year award for 2017 Manufacturing ... the event was hosted by CompanyWeek and Manufacturers Edge, among other sponsors. ...
(Date:4/21/2017)... , ... April 21, 2017 , ... ... Webster Bank, today announced first round funding to three startups through the UConn ... financial support to new business startups affiliated with UConn. , The UConn Innovation ...
(Date:4/21/2017)... Muncie, IN (PRWEB) , ... April 21, 2017 , ... ... year to 11 high school graduates from across the nation. The scholarships are created ... of AMA member dues. , Scholarship criteria are set by the AMA Scholarship Committee, ...
(Date:4/21/2017)... ... April 21, 2017 , ... Frederick ... a range of emerging technology-based businesses, recently earned a $77,518 grant from the ... , Founded in 2004, FITCI is Frederick’s first incubator. A non-profit corporation, FITCI ...
Breaking Biology Technology: