Navigation Links
NYSCF scientists develop new protocol to ready induced pluripotent stem cell clinical application
Date:4/3/2013

NEW YORK, NY (April 3, 2013) A team of New York Stem Cell Foundation (NYSCF) Research Institute scientists led by David Kahler, PhD, NYSCF Director of Laboratory Automation, have developed a new way to generate induced pluripotent stem (iPS) cell lines from human fibroblasts, acquired from both healthy and diseased donors. Reported in PLOS ONE, this cell-sorting method consistently selects the highest quality, standardized iPS cells, representing a major step forward for drug discovery and the development of cell therapies.

Employing a breakthrough method developed by 2012 Nobel laureate Shinya Yamanaka, MD, PhD, adult cells are "reprogrammed" or reverted to an embryonic-like state, commonly through viral infection. Reprogramming is a dynamic process, resulting in a mixture of fully reprogrammed iPS cells, partially reprogrammed cells, and residual adult cells. Previous protocols to select promising fully reprogrammed cells rely primarily on judging stem cell colonies by eye through a microscope.

Cell colonies selected by qualitative measures could include partially reprogrammed cells, a major concern for clinical applications of cell therapies because these cells could become any other cell type in a patient following transplantation. Additionally for drug efficacy assays and toxicity investigations on iPS cells, heterogeneous cell populations can mar the response of representative iPS cell lines.

The NYSCF scientists developed a quantitative protocol, optimized over three and a half years, in order to consistently harvest early-reprogrammed cells. Using fluorescence activated cell sorting (FACS), fully reprogrammed cells were identified by two specific proteins, or pluripotency markers. The group then looked at third marker that is expressed by partially reprogrammed or adult cells, and they then negatively selected against these cells to obtain only fully reprogrammed cells.

"To date, this protocol has enabled our
'/>"/>

Contact: David McKeon
dmckeon@nyscf.org
212-365-7440
New York Stem Cell Foundation
Source:Eurekalert

Page: 1 2

Related biology news :

1. NYSCF scientists develop 3-D stem cell culture technique to better understand Alzheimers disease
2. Scientists find government justification of new environmental policy unfounded
3. UGA discovery may allow scientists to make fuel from CO2 in the atmosphere
4. UT MD Anderson scientists uncover the nuclear life of actin
5. Scientists awarded £3M to study the way Northwest European seas absorb carbon
6. Scientists reveal quirky feature of Lyme disease bacteria
7. Scientists create new tools for battling secondhand smoke
8. Academy scientists receive top honors for long-term research and training initiatives in Mongolia
9. Scientists discover reasons behind snakes shrinking heads
10. U of T scientists map genome that causes Dutch Elm Disease
11. Scientists discover novel chemical that controls cell behavior
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/27/2015)... 27, 2015  For more than four decades, AUVSI,s ... been the premier worldwide event for the unmanned land, ... must-attend event for any local, national, or trade news ... commercial markets and current applications of unmanned technologies. The ... projected $48 billion industry, and how it will soon ...
(Date:4/20/2015)... PASADENA, Calif. , April 20, 2015 ... San Gabriel Valley to implant a new miniaturized, wireless ... HF System is the first and only FDA-approved heart ... reduce hospital admissions when used by physicians to manage ... a sensor that is implanted in the pulmonary artery ...
(Date:4/14/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" ... the growing mobile commerce market, announces that its Wocket® ... York Design Awards under the category  ,Product Design – ... Awards are part of a global multi-disciplinary awards program. ... the marketplace, industry and judging panel. Winners will be ...
Breaking Biology News(10 mins):UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 2UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 3UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 4UNMANNED SYSTEMS 2015: Largest International Conference and Trade Show for the Drone and Unmanned Technology Industry 5Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 2NXT-ID's Wocket Smart Wallet Nominated for 2015 New York Design Awards in 'Product Design- Technology' 3
... 19, 2012 Many biotech observers maintain that the ... the field of personalized medicine, reports Genetic ... ( http://genengnews.com ). Although drug discovery efforts require ... is exceeding supply, fueling the growth of the ...
... team, led by Institute of Oceanology of Chinese Academy ... and analysis of Pacific oyster (Crassostrea gigas) genomethe first ... a void in our understanding of the species-rich but ... in Nature , reveals the unique adaptations of ...
... Oregon State University for the first time have confirmed some ... help destroy coral reefs it appears they allow an ... choke off oxygen and disrupt helpful bacteria. These "macroalgae," ... corals. They can get out of control when sewage increases ...
Cached Biology News:Oyster genome uncover the stress adaptation and complexity of shell formation 2Oyster genome uncover the stress adaptation and complexity of shell formation 3The 'slippery slope to slime': Overgrown algae causing coral reef declines 2
(Date:5/27/2015)... MA (PRWEB) May 27, 2015 The ... is directing, via its wholly owned subsidiary, Anelixis ... potential treatment for ALS that targets the innate immune ... ALS Therapy Development Institute received from the 2014 social ... The entire sum of $4 million raised directly by ...
(Date:5/27/2015)... Calif. , May 27, 2015  Two years ... biotech professionals, hackers and citizen scientists, Oakland ... $30,000 crowdfunding campaign promoting its mission to democratize science ... Temescal District. ... Based out of ...
(Date:5/27/2015)... Madison, NJ (PRWEB) May 27, 2015 ... for evaluating the impact of regulation on medical innovation, ... Centered Innovation (iPCI) of Fairleigh Dickinson University’s ( ... inaugural MI3 Alert pertains to new FDA ... Senate proposal for accelerating the approval of certain medical ...
(Date:5/26/2015)... WORMS, GERMANY – May 26, ... GRA) announces that its manufacturing facility in Worms, ... from EXCiPACT™, an independent organization that certifies manufacturers, ... , All three Grace facilities that produce its ... gels have now received GMP certification, following the ...
Breaking Biology Technology:Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 2Ice Bucket Challenge Advances Antibody Toward Clinical Trial for ALS 3East Bay's Counter Culture Labs Seeks to Open Doors of Science to Public 2East Bay's Counter Culture Labs Seeks to Open Doors of Science to Public 3Medical Innovation Impact Index Launched at Fairleigh Dickinson University’s Initiative for Patient-Centered Innovation (iPCI) under the Rothman Institute 2CORRECTION: Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2
... by a young researcher to leave the University of ... the same-sex union debate in Wisconsin. , ,Rob Carpick, an ... million in grants since coming to Madison in 2000. At ... and research with him to the University of Pennsylvania ...
... crowd. I got pummeled this week in comments and e-mails for ... I just can't call a phone, and I'm sure that's going ... at shutupanddrive.org makes his position clear, took me to task for ... talk on my mobile phone while driving. Angus is correct and ...
... and all the IP-driven features, and what broadband speeds are ... competitiveness, economic development equals broadband connectivity and broadband connectivity equals ... speed. Thats all the consumer needs to know. In fact, ... Project Lightspeed offers data speeds that are nothing ...
Cached Biology Technology:Departure of nanotech researcher stings UW 2Departure of nanotech researcher stings UW 3Am I spoiled if I expect well-designed mobile devices? 2Am I spoiled if I expect well-designed mobile devices? 3Broadband blues: Don't fall for Lightspeed hype 2Broadband blues: Don't fall for Lightspeed hype 3Broadband blues: Don't fall for Lightspeed hype 4Broadband blues: Don't fall for Lightspeed hype 5
... The Multiple Species Tissue Array (MSTA) slides were ... cells and tissues from multiple species. Tissue ... each MSTA slide which can then be treated ... immunohistochemistry, or in situ hybridization. ...
... Clear yellow solution. ... 0.01% thimerosal, and other ... filtered. Diluent formulated to ... peroxidase antibody conjugates at ...
... cells-directed transfection reagent : jetPEI-RGD ... which allows targeted efficient transfection ... cells. Integrins are adhesion molecules ... adhesion processes. Many integrins, including ...
...
Biology Products: