Navigation Links
NIST calculations may improve temperature measures for microfluidics
Date:9/9/2009

roposed by NIST researchers David Ross, Michael Gaitan and Laurie Locascio in 2001*). But it turns out that the curves are only good for that one temperature. In an upcoming paper in Analytical Chemistry**, the NIST teamJayna J. Shah, Michael Gaitan and Jon Geistreports that changing the reference point, such as the higher temperature when a microfluidic system is first heated, introduces errors when a dye intensity-to-temperature calculation is done using current methods.

"Our analysis shows that a simple linear correction for a 40 degrees Celsius reference temperature identified errors between minus 3 to 8 degrees Celsius for three previously published sets of calibration equations derived at approximately 23 degrees Celsius," says lead researcher Shah.

To address the problem, the NIST team developed mathematical methods to correct for the shift experienced when the reference temperature changes. This allowed the researchers to create generalized calibration equations that can be applied to any reference temperature.

Microfluidic DNA amplification (production of numerous copies of DNA from a tiny sample) by the polymerase chain reaction (PCR) is one procedure that could benefit from the new NIST calculations, Shah says. "PCR requires a microfluidic device to be cycled through temperatures at three different zones starting around 65 degrees Celsius, so a useful dye intensity-to-temperature ratio would have to be based on that temperature and not a reference point of 23 degrees Celsius," she explains.


'/>"/>

Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert

Page: 1 2

Related biology news :

1. New Iowa State supercomputer, Cystorm, unleashes 28.16 trillion calculations per second
2. Shortcuts of the mind lead to miscalculations of weight and caloric intake, says Penn study
3. Carnegie Mellon urges industry to broaden carbon footprint calculations
4. Icy calculations on a hot topic
5. A breath of fresh air could improve drug toxicity screening
6. U-Iowa improves delivery of cancer-fighting molecules
7. Novel polymer could improve protein-based drugs
8. Water quality improves after lawn fertilizer ban, study shows
9. Improved air quality during Beijing Olympics could inform pollution-curbing policies
10. Technology improves salmon passage at hydropower dams
11. Short stressful events may improve working memory
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/21/2015)... , May 21, 2015 ... "Thermal Imaging Market by Solutions (Hardware, Software, Services), by ... Automotive & others), by End-Users (Military & Defense ... by MarketsandMarkets, Thermal Imaging Market is expected to ... Million by 2020, at a Compound Annual Growth ...
(Date:5/21/2015)... May 21, 2015 The Sync Project™ ... music for health, today announced a collaborative partnership with ... partnership will center on collaboration on original research, joint ... step in the collaboration, The Sync Project and BerkleeICE ... as Medicine" in the 2015-2016 academic year.  ...
(Date:5/20/2015)... DUBLIN , May 20, 2015 ... Markets ( http://www.researchandmarkets.com/research/h8t4x6/5year ) has announced the ... of the Global Iris Recognition Market" ... http://photos.prnewswire.com/prnh/20130307/600769) , ,This research service analyses ... across the government and commercial sectors, ...
Breaking Biology News(10 mins):Thermal Imaging Market Worth $9,998.9 Million by 2020 2Thermal Imaging Market Worth $9,998.9 Million by 2020 3Thermal Imaging Market Worth $9,998.9 Million by 2020 4The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 2The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 3Global Iris Recognition Market Report 2015 - 5-Year Opportunity Analysis 2
... Scientists have used mass spectrometry for decades to ... has it been used to identify viruses, and ... at the Johns Hopkins Bloomberg School of Public ... the potential to be applied for this purpose. ...
... thwarts a disease-causing invader that creates a fuzzy gray ... gene provides access to a different type of pathogen. ... collaborators in Austria and North Carolina identified the gene ... defense against disease. The gene and its defense mechanisms ...
... today finds that the illegal trade in the Roti Island snake-necked ... all but extinct in the wild. Exotic pet enthusiasts in ... for the turtle, often without realizing that they are contributing to ... allowed since 2001. , The report by TRAFFIC, the ...
Cached Biology News:Researchers use mass spectrometry to detect norovirus particles 2Gene thwarts some pathogens, gives access to others, could save crops 2Gene thwarts some pathogens, gives access to others, could save crops 3Illegal trade is propelling rare turtle toward extinction, new report 2
(Date:5/22/2015)... 22, 2015 According to a ... & 2D Gel Electrophoresis, Agarose & Polyacrylamide Gel, Capillary ... by Application & by End User - Global Forecast ... expected to reach around $1.98 Billion by 2020 with ... to 2020. Browse 226 ma ...
(Date:5/22/2015)... (PRWEB) May 22, 2015 Charm ... U.S. Department of Agriculture (USDA), Grain Inspection, Packers ... year contract to Charm Sciences to monitor aflatoxin ... Test (water-based) and ROSA FAST Aflatoxin Quantitative Test ... Quantitative Test uses Water Extraction Technology to ...
(Date:5/22/2015)... Knowledgent , the data and analytics firm, ... of big data analytics in the life sciences and ... life sciences and healthcare organizations understand the ways in ... insights. , The life sciences and healthcare industries historically ... profiling, compliance and regulatory requirements, and scientific research. These ...
(Date:5/22/2015)... Baylor Research Institute (BRI) at Dallas ... in Phoenix, AZ , today announce an ... for patients with a broad range of cancers. Aligning the ... trials and access to technology to drive the development of ... across Baylor Scott & White Health,s 49 hospitals ...
Breaking Biology Technology:Electrophoresis Market Worth $1.98 Billion by 2020 2Electrophoresis Market Worth $1.98 Billion by 2020 3Electrophoresis Market Worth $1.98 Billion by 2020 4USDA-GIPSA (FGIS) Awards 5 Year Contract for Aflatoxin Tests to Charm Sciences 2New White Paper Explores the Potential of Big Data Analytics in Life Sciences and Healthcare 2Precision Medicine Collaboration to Increase Options for Cancer Patients 2Precision Medicine Collaboration to Increase Options for Cancer Patients 3Precision Medicine Collaboration to Increase Options for Cancer Patients 4
... 5 Cord Blood America, Inc. (OTC Bulletin Board: ... ( http://www.cordblood-america.com ) focused on bringing the life ... internationally, said today that Matthew Schissler, founder and CEO, ... the Company is very close to becoming cash flow ...
... MITI ), today announced that its President and CEO, Dr. Christian ... Waldorf-Astoria in New York City on Tuesday, February 10, 2009. A simultaneous ... www.micromet-inc.com ., Forum: The BIO ... February 10, 2009, Time: 2:15 ...
... Feb. 5 Nutrition 21, Inc. (Nasdaq: ... under the Iceland Health(R), Chromax(R) and Diabetes Essentials(R) brands ... health, enhance memory and reduce chronic joint pain, today ... first half of fiscal year 2009.For the quarter the ...
Cached Biology Technology:In Analyst Interview, Cord Blood America Says Stem Cells Now Front and Center for Researchers and Investors 2Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 2Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 3Micromet to Present at the BIO CEO and Investor Conference on February 10, 2009 4Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 2Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 3Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 4Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 5Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 6Nutrition 21 Announces Second Consecutive Profitable Quarter in Q2 Fiscal 2009 7
... The MyCycler thermal cycler system is ... applications. This system includes the MyCycler personal ... module for use with 0.2 ml tubes ... options, and both algorithm and block modes ...
... have been developed for affinity capture ... variety of ProteinChip chemical surfaces allow ... their unique biochemical properties. Each chip ... sample comparison, and the chips are ...
The Freeze 'N Squeeze DNA gel extraction kit uses a quick freeze-and-thaw cycle and centrifugation to draw 50-23,000 bp DNA out of agarose gel slices. The kit includes materials for 100 preps....
The Econo gradient pump rack is a preassembled rack that is used to hold the components of the Econo gradient pump kits....
Biology Products: