Navigation Links
NIST calculations may improve temperature measures for microfluidics
Date:9/9/2009

roposed by NIST researchers David Ross, Michael Gaitan and Laurie Locascio in 2001*). But it turns out that the curves are only good for that one temperature. In an upcoming paper in Analytical Chemistry**, the NIST teamJayna J. Shah, Michael Gaitan and Jon Geistreports that changing the reference point, such as the higher temperature when a microfluidic system is first heated, introduces errors when a dye intensity-to-temperature calculation is done using current methods.

"Our analysis shows that a simple linear correction for a 40 degrees Celsius reference temperature identified errors between minus 3 to 8 degrees Celsius for three previously published sets of calibration equations derived at approximately 23 degrees Celsius," says lead researcher Shah.

To address the problem, the NIST team developed mathematical methods to correct for the shift experienced when the reference temperature changes. This allowed the researchers to create generalized calibration equations that can be applied to any reference temperature.

Microfluidic DNA amplification (production of numerous copies of DNA from a tiny sample) by the polymerase chain reaction (PCR) is one procedure that could benefit from the new NIST calculations, Shah says. "PCR requires a microfluidic device to be cycled through temperatures at three different zones starting around 65 degrees Celsius, so a useful dye intensity-to-temperature ratio would have to be based on that temperature and not a reference point of 23 degrees Celsius," she explains.


'/>"/>

Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert

Page: 1 2

Related biology news :

1. New Iowa State supercomputer, Cystorm, unleashes 28.16 trillion calculations per second
2. Shortcuts of the mind lead to miscalculations of weight and caloric intake, says Penn study
3. Carnegie Mellon urges industry to broaden carbon footprint calculations
4. Icy calculations on a hot topic
5. A breath of fresh air could improve drug toxicity screening
6. U-Iowa improves delivery of cancer-fighting molecules
7. Novel polymer could improve protein-based drugs
8. Water quality improves after lawn fertilizer ban, study shows
9. Improved air quality during Beijing Olympics could inform pollution-curbing policies
10. Technology improves salmon passage at hydropower dams
11. Short stressful events may improve working memory
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/22/2014)... Dec. 22, 2014  The 2014 Holiday Season may be ... Market Intelligence reports that the long anticipated floodgates for ... intensifying demand for smart phones, tablets, and wearable mobile ... of 2.5 billion users with nearly 4.8 billion biometric ...
(Date:12/19/2014)... , Dec. 18, 2014 Research and Markets ( ... Market Monitor: North America Perimeter Security Systems Market" report ... The North American perimeter security market is estimated ... 2019. Although the U.S. market holds a larger share in ...
(Date:12/19/2014)... , Dec. 18, 2014   LaunchKey , the ... the post-password and Internet of Things era, today announced ... The venture round was led by Metamorphic Ventures with ... Venture Partners, VegasTechFund, and others.  LaunchKey has raised $4 ...
Breaking Biology News(10 mins):First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3
... diseases can rival malaria,s ability to kill and cripple. Each year ... and more than a million mostly African children under the ... anniversary of the announcement of the genome sequence of the deadly ... Anopheles . A new book on malaria ...
... shelves until Oct. 2, it has already passed an enviable ... 100 books of all time. And they fit on ... of Genetics at Harvard Medical School and a founding core ... Harvard University, and his team encoded the book, Regenesis: How ...
... as carbon drains but are far from places where greenhouses ... has proposed human, agricultural and livestock waste, such as urine, ... large quantities of carbon dioxide and other greenhouse gases present ... occurring substance. A study published in the Journal of ...
Cached Biology News:New book tells story of 10-year-old malaria project 2New book tells story of 10-year-old malaria project 3Writing the book in DNA 2Writing the book in DNA 3A urine based 'potion' can act as a CO2 absorbent 2A urine based 'potion' can act as a CO2 absorbent 3
(Date:1/22/2015)... BOSTON , Jan. 22, 2015  Transwestern | RBJ today ... feet of office space for Shire a leading biopharmaceutical company, ... . Transwestern | RBJ,s Robert Richards , president, and ... the lease for the entire five-floor building at 95 Hayden ...
(Date:1/22/2015)... 2015 Protocol Networks brings independent technology ... of brand-neutral, independent consultants, Protocol Networks has recently announced ... over the years, his company has attracted several clients ... Plainfield, as well as others. With the success of ...
(Date:1/22/2015)... Boston, MA (PRWEB) January 22, 2015 ... systems, is pleased to announce an exciting product from a ... of motion analysis solutions over the past 15 years, with ... industries. Most recently, their 3D IMU system (inertial measurement unit), ...
(Date:1/22/2015)... Selexis SA, a serial innovation company with ... used for drug discovery to commercial manufacturing, announced today ... Next-Generation Sequencing (NGS) data packages. The NGS analysis ... ensuring the integrity of the gene, validation of the ...
Breaking Biology Technology:Transwestern?RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Protocol Networks Brings Independent Technology Consulting to the Constitution State 23D Motion Capture Just Got Easy 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2
... 2008--Researchers at the Gladstone Institute of Cardiovascular Disease (GICD) ... for the first time how tiny genetic factors called ... (ES) cells into cardiac muscle. As reported in the ... GICD Director, Deepak Srivastava, MD, demonstrated that two microRNAs, ...
... ACUR ) reported 2007 fourth quarter net,income of $9.5 ... $3.9 million, or $0.44 loss per diluted share (after giving ... quarter in 2006.,For the year ended December 31, 2007, the ... share compared to a net loss of $6.0 million, or,$0.75 ...
... drug and diagnostic discovery company, today announces its,consolidated financial ... 2007 Key Events, - Milestone reached in the ... in view of the initiation of Phase 1 trials ... Approval by the AFSSAPS (Agence Francaise de Securite Sanitaire ...
Cached Biology Technology:Gladstone scientists identify role of tiny RNAs in controlling stem cell fate 2Acura Pharmaceuticals, Inc. Reports 2007 4th Quarter and Annual Financial Results; Sets 2008 Expectations 2Acura Pharmaceuticals, Inc. Reports 2007 4th Quarter and Annual Financial Results; Sets 2008 Expectations 3Acura Pharmaceuticals, Inc. Reports 2007 4th Quarter and Annual Financial Results; Sets 2008 Expectations 4Acura Pharmaceuticals, Inc. Reports 2007 4th Quarter and Annual Financial Results; Sets 2008 Expectations 5Acura Pharmaceuticals, Inc. Reports 2007 4th Quarter and Annual Financial Results; Sets 2008 Expectations 6ExonHit Therapeutics - 2007 Financial Results 2ExonHit Therapeutics - 2007 Financial Results 3ExonHit Therapeutics - 2007 Financial Results 4ExonHit Therapeutics - 2007 Financial Results 5ExonHit Therapeutics - 2007 Financial Results 6ExonHit Therapeutics - 2007 Financial Results 7ExonHit Therapeutics - 2007 Financial Results 8ExonHit Therapeutics - 2007 Financial Results 9ExonHit Therapeutics - 2007 Financial Results 10ExonHit Therapeutics - 2007 Financial Results 11ExonHit Therapeutics - 2007 Financial Results 12
Recombinant Mouse TGF-beta RII/(human) Fc Chimera, CF...
Human Norrin Biotinylated Affinity Purified PAb Protein Family: Other Growth Factors, Other Wnt-related Molecules...
...
Recombinant Mouse Matrilin-4, CF Protein Family: Extracellular Matrix Molecules, Chondrogenesis Markers...
Biology Products: