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NIST calculations may improve temperature measures for microfluidics
Date:9/9/2009

If you wanted to know if your child had a fever or be certain that the roast in the oven was thoroughly cooked, you would, of course, use a thermometer that you trusted to give accurate readings at any temperature within its range. However, it isn't that simple for researchers who need to measure temperatures in microfluidic systemstiny, channel-lined devices used in medical diagnostics, DNA forensics and "lab-on-a-chip" chemical analyzersas their current "thermometer" can only be precisely calibrated for one reference temperature. Now, researchers at the National Institute of Standards and Technology (NIST) have proposed a mathematical solution that enables researchers to calibrate the "thermometer" for microfluidic systems so that all temperatures are covered.

Reactions taking place in microfluidic systems often require heating, meaning that users must accurately monitor temperature changes in fluid volumes ranging from a few microliters (a droplet approximately 1 millimeter in diameter) to sub-nanoliters (a droplet approximately 1/10 of millimeter in diameter). A common DNA analysis technique, for example, depends heavily on precise temperature cycling. Ordinary thermometers or other temperature probes are useless at such tiny dimensions, so some groups have turned to temperature-sensitive fluorescent dyes, particularly rhodamine B. The intensity of the dye's fluorescence decreases with increasing temperature. The idea is that the dye can be used as a noninvasive way to map the range of temperatures occurring within a microfluidic system during heating and, in turn, provide a means of calibrating that system for experiments.

However, the technique currently requires the user to base all readings on the fluorescence at a single reference temperature. Previous groups have developed "calibration curves" that relate temperature to rhodmaine B fluorescent intensity based on a reference temperature of about 23 degrees Celsius (a technique first p
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Contact: Michael E. Newman
michael.newman@nist.gov
301-975-3025
National Institute of Standards and Technology (NIST)
Source:Eurekalert

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