Navigation Links
Monitoring and robust induction of nephrogenic intermediate mesoderm from human iPSCs
Date:1/25/2013

The research group led by Associate Professor Kenji Osafune and his colleague Shin-ichi Mae, both from Center for iPS Cell Research and Application (CiRA), Kyoto University in Japan, has succeeded in developing a highly efficient method of inducing human induced pluripotent stem (iPS) cells to differentiate into intermediate mesoderm, the precursor of kidney, gonad, and other cell lineages. This represents a major step toward realizing renal regeneration.

As nearly all kidney cells are derived through differentiation from intermediate mesoderm, to realize kidney regeneration requires first the development of an efficient technology for differentiating human iPS or embryonic stem (ES) cells into intermediate mesoderm.

The research team established a method through which fluorescent protein can be readily inserted into the human iPS/ES cell genome through homologous recombination and used it in human iPS cells to successfully introduce green fluorescent protein (GFP) into Odd-skipped related 1: (OSR1), a marker gene for intermediate mesoderm differentiation. This makes it possible to ascertain whether differentiation into the target intermediate mesoderm cells has been achieved.

The system was then used to establish a protocol for inducing iPS cell differentiation into intermediate mesoderm which produced a high success rate of 90% or more. It was confirmed that the resulting human intermediate mesoderm was able to differentiate into various types of kidney cell, and renal tubule structures were successfully generated.

The findings indicate the possibility of using iPS cells to create a supply of cells for use in renal regenerative medicine. The differentiation system developed by the researchers is also expected to provide a new research tool to help elucidate the developmental mechanism of intermediate mesoderm.

The next step required is to develop a technique that allows efficient and specific differentiation in
'/>"/>

Contact: CiRA International Public Communications Office
cira-pr@cira.kyoto-u.ac.jp
81-753-667-000
Center for iPS Cell Research and Application - Kyoto University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Chemical pollution in Europes seas: The monitoring must catch up with the science
2. New CU-NOAA monitoring system clarifies murky atmospheric questions
3. Tattoo-like devices for wireless pregnancy monitoring
4. Preventice Expands Options for Wireless Patient Monitoring with Qualcomm Life
5. NTU start-up launches worlds first 3-in-1 water monitoring system
6. Forest carbon monitoring breakthrough in Colombia
7. Ecological monitoring on bird populations in Europe re-evaluated
8. Sandia shows monitoring brain activity during study can help predict test performance
9. Detection, analysis of cell dust may allow diagnosis, monitoring of brain cancer
10. Call for global monitoring of infectious diseases in dogs and cats
11. Wildlife monitoring cameras click jaguar and ocelot photos
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/25/2014)... Wash.Five years ago this month, one of the first ... Washington State University campus, striking some 2,000 people. A ... of data gathered at the time to gain insight ... the virus,s rapid spread across the university community., The ... cases came in to the university,s Health and Wellness ...
(Date:8/25/2014)... battery-powered DNA diagnostic device invented at New Zealand,s ... commonly used field tool for rapidly detecting suspected ... the level of infection. , The breakthrough device, ... Queenstown Molecular Biology main meeting. It takes advantage ... target DNA sequences in real-time, without the need ...
(Date:8/25/2014)... a device the size of a cell phone may ... according to a team of engineers., "For biological testing ... said Tony Jun Huang, professor of engineering science and ... of the cells, damages them in any way, the ... waves can separate cells using very small amounts of ...
Breaking Biology News(10 mins):WSU flu outbreak provides rare study material 2WSU flu outbreak provides rare study material 3Revolutionary handheld DNA diagnostic unit allows lab-quality analysis in the field 2Tilted acoustic tweezers separate cells gently 2
... for many people with depression and anxiety disorders, and ... care providers, according to researchers who analyzed the results ... shown to have tremendous benefits for mental health," says ... Program at Southern Methodist University in Dallas. "The more ...
... at Ume University and the Sahlgrenska Academy at the University ... can be found in fish that have been swimming in ... found in higher concentrations in the blood of fish than ... this hormone can lead to infertility in fish. The ...
... neurodegenerative disease spinocerebellar ataxia type 5 (SCA5) damages nerve ... report that the defective protein responsible for the disease ... inside neurons. The study appears in the April 5 ... ( www.jcb.org ). SCA5 results from a faulty ...
Cached Biology News:Mental health providers should prescribe exercise more often for depression, anxiety 2Medicine residues may threaten fish reproduction 2Defective protein is a double hit for ataxia 2
(Date:8/26/2014)... San Francisco, CA (PRWEB) August 26, 2014 ... to reach USD 8,020.1 million by 2020, according to ... demand for personalized medicine and theranostics, and the subsequent ... be key factors driving market growth over the next ... population and chronic diseases such as cancer, coupled with ...
(Date:8/26/2014)... Genedata, a leading provider of ... science research, today announced the Genedata Screener® ... in Asia, Europe, and North America during September ... leading research organizations will gather for annual User ... and upcoming challenges in all areas of screening. ...
(Date:8/26/2014)... 26, 2014   Loveland Products, a subsidiary of ... crop input products and proprietary products provider for ... has acquired a controlling interest in Agricen, a ... delivering biochemical-based products for efficient and sustainable plant ... growing plant nutrition brands, Accomplish ® LM ...
(Date:8/26/2014)... -- That,s Nice LLC, a research-focused strategic marketing agency dedicated ... a new dedicated team that will support clients with ... current and new staff in both market research and ... Guy Tiene has been hired as Director ... previously Director of Marketing for Royal DSM,s Pharmaceutical Products ...
Breaking Biology Technology:World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 2World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 3World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 4World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 5World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 6Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 2Thought Leaders to Explore Innovations in SPR & Combination Screening at Genedata Screener User Group Meetings 3Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 2Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 3Agrium, through Loveland Products, Acquires a Controlling Interest in Agricultural Biotechnology Company Agricen 4That's Nice Announces Expanded Offering For Strategic Content And Public Relations 2That's Nice Announces Expanded Offering For Strategic Content And Public Relations 3
... just the expected crowd of educators is congregating at ... Conference that runs through Friday; as the technologies ... fields involved. In addition to K-12 and higher education ... and military organizations. , ,Distance Learning 2005 is addressing ...
... The Centers for Medicare and Medicaid Services plan to officially ... Vista within the next few weeks, according to news accounts ... ,CMS officials hope that providing the new program, known as ... records by private clinicians. , ,Vista, a software program used ...
... Ann Stunden, director of the Division of Information Technology ... 2005 award for information technology leadership from the national ... for "strong and ground-breaking leadership in the information technology ... ago." , ,A co-founder and executive director of ...
Cached Biology Technology:Education conference focusing on importance of integrating technology 2Education conference focusing on importance of integrating technology 3Education conference focusing on importance of integrating technology 4
Identify and characterize Phosphoinositide (PI(4,5)P2) binding...
... Immunogen corresponds to amino acids 2-105 from ... been successfully used in Western blot, immunocytochemistry ... this antibody detects an ~ 60 kDa ... also been used in immunoprecipitation and immunocytochemical ...
Caspr2 Antigen: Fusion protein amino acids 96-1265 (extracellular domain) of human Caspr2 Accession Number: NP_054860...
... Our Accumix standard is a new tuning ... electrospray ionization mass spectrometry. Accumix is ... mass range coverage for both tuning and ... and LTQ-MS) and Fourier transform mass spectrometers. ...
Biology Products: