Navigation Links
Monitoring and robust induction of nephrogenic intermediate mesoderm from human iPSCs
Date:1/25/2013

The research group led by Associate Professor Kenji Osafune and his colleague Shin-ichi Mae, both from Center for iPS Cell Research and Application (CiRA), Kyoto University in Japan, has succeeded in developing a highly efficient method of inducing human induced pluripotent stem (iPS) cells to differentiate into intermediate mesoderm, the precursor of kidney, gonad, and other cell lineages. This represents a major step toward realizing renal regeneration.

As nearly all kidney cells are derived through differentiation from intermediate mesoderm, to realize kidney regeneration requires first the development of an efficient technology for differentiating human iPS or embryonic stem (ES) cells into intermediate mesoderm.

The research team established a method through which fluorescent protein can be readily inserted into the human iPS/ES cell genome through homologous recombination and used it in human iPS cells to successfully introduce green fluorescent protein (GFP) into Odd-skipped related 1: (OSR1), a marker gene for intermediate mesoderm differentiation. This makes it possible to ascertain whether differentiation into the target intermediate mesoderm cells has been achieved.

The system was then used to establish a protocol for inducing iPS cell differentiation into intermediate mesoderm which produced a high success rate of 90% or more. It was confirmed that the resulting human intermediate mesoderm was able to differentiate into various types of kidney cell, and renal tubule structures were successfully generated.

The findings indicate the possibility of using iPS cells to create a supply of cells for use in renal regenerative medicine. The differentiation system developed by the researchers is also expected to provide a new research tool to help elucidate the developmental mechanism of intermediate mesoderm.

The next step required is to develop a technique that allows efficient and specific differentiation in
'/>"/>

Contact: CiRA International Public Communications Office
cira-pr@cira.kyoto-u.ac.jp
81-753-667-000
Center for iPS Cell Research and Application - Kyoto University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Chemical pollution in Europes seas: The monitoring must catch up with the science
2. New CU-NOAA monitoring system clarifies murky atmospheric questions
3. Tattoo-like devices for wireless pregnancy monitoring
4. Preventice Expands Options for Wireless Patient Monitoring with Qualcomm Life
5. NTU start-up launches worlds first 3-in-1 water monitoring system
6. Forest carbon monitoring breakthrough in Colombia
7. Ecological monitoring on bird populations in Europe re-evaluated
8. Sandia shows monitoring brain activity during study can help predict test performance
9. Detection, analysis of cell dust may allow diagnosis, monitoring of brain cancer
10. Call for global monitoring of infectious diseases in dogs and cats
11. Wildlife monitoring cameras click jaguar and ocelot photos
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/18/2014)... a sample of patients with undiagnosed, suspected genetic conditions, ... with a higher molecular diagnostic yield than traditional molecular ... JAMA . The study is being released to coincide ... , Exome sequencing, which sequences the protein­coding region of ... material present in a cell or organism), has been ...
(Date:10/17/2014)... in German . ... day in order to reproduce? And why are there two ... the latest issue of the research journal Molecular Human ... Steven Ramm from Bielefeld University Bielefeld has compiled this special ... for a female to copulate with several males in quick ...
(Date:10/17/2014)... German . ... When treating overdoses, doctors are often limited to supportive therapy ... is a combination of drugs involved. So what can be ... grandmother,s pills? ETH professor Jean-Christophe Leroux from the Institute of ... to this question. "The task was to develop an agent ...
Breaking Biology News(10 mins):Study examines type of exome sequencing and molecular diagnostic yield 2Study examines type of exome sequencing and molecular diagnostic yield 3Sperm wars 2Emergency aid for overdoses 2Emergency aid for overdoses 3
... - Animals that choose to eat in the presence of a ... go into a defensive mode and pay a physical penalty for ... crop pest hornworm caterpillar, a study shows. While other animals ... hornworm caterpillars slow or stop eating but actually keep up their ...
... to participate in ELIXIR ( www.elixir-europe.org ), a major ... in Europe. With one of Europe,s larger economies now ... is well placed to continue its excellent progress. ... infrastructure for managing biological data to support research and ...
... the IRCM, led by geneticist Dr. Jacques Drouin, recently ... inflammation. This important breakthrough will be published in tomorrow,s ... . IRCM scientists study glucocorticoids, a class ... reduce inflammation. They are used in medicine to treat ...
Cached Biology News:Caterpillar gets more from its food when predator is on the prowl 2Italy pledges to be part of ELIXIR research infrastructure 2Controlling inflammatory and immune responses 2
(Date:10/20/2014)... 20, 2014 Asterias Biotherapeutics, Inc. (NYSE ... signed a Notice of Grant Award (NGA) with ... October 1, 2014.  The NGA provides for the ... release of additional grant funds pursuant to the ... clinical development of Asterias, product, AST-OPC1. The grant ...
(Date:10/20/2014)... OncLive® is pleased ... at Thomas Jefferson University has joined its Strategic ... Alliance Partnership program, the Sidney Kimmel Cancer Center ... awareness of the Center’s cutting-edge research programs, comprehensive ... and other health care professionals from the Sidney ...
(Date:10/19/2014)... , Oct. 19, 2014 NextCODE Health, which enables ... genome in real time, today announced the launch of its ... Exchange , at the American Society of Human Genetics (ASHG) ... learn more, sign up and apply for free beta access, ... features and benefits can be viewed here . ...
(Date:10/19/2014)... 19, 2014 The Asian Orthopedic braces and ... Asia with analysis and forecast of revenue. The Orthopedic braces ... to around $416.5 million by 2018, at a developing CAGR ... TOC of the Asian Orthopedic braces and support systems market, ... also provides a glimpse of the segmentation of orthopedic braces ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 2The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 3The Asian Orthopedic braces and support systems market is estimated to grow to around $416.5 million by 2018 - New Report by MicroMarket Monitor 4
... in Revenue and Positive ... EBITDA for Three Consecutive Quarters, PRINCETON, N.J., April 1 ... production company, today,announced record growth in revenues for 2007. The ... and generated positive EBITDA for,the third consecutive quarter. Laureate achieved ...
... FREMONT, Calif., April 1, 2008 WaferGen,Biosystems, ... developer of,state-of-the-art gene expression, genotyping, cell biology ... signing of an exclusive distribution,agreement for the ... in Germany. The distributor agreement covers WaferGen,s,entire ...
... 2008 Plethora Solutions Holdings PLC,("Plethora", AIM: PLE), ... management of urological disorders, today announced that it,has ... Paul Capital,Healthcare. Through this agreement, Plethora will receive ... payable upon signature and a further $10 million,upon ...
Cached Biology Technology:Laureate Pharma Reports Record Growth for 2007 2Laureate Pharma Reports Record Growth for 2007 3WaferGen Signs Exclusive Distribution Agreement for SmartSlide(TM) Micro-Incubation System in Germany 2WaferGen Signs Exclusive Distribution Agreement for SmartSlide(TM) Micro-Incubation System in Germany 3WaferGen Signs Exclusive Distribution Agreement for SmartSlide(TM) Micro-Incubation System in Germany 4WaferGen Signs Exclusive Distribution Agreement for SmartSlide(TM) Micro-Incubation System in Germany 5Plethora and Paul Capital Healthcare sign a $25 million revenue interest financing agreement with an option for an additional $3 million equity investment 2Plethora and Paul Capital Healthcare sign a $25 million revenue interest financing agreement with an option for an additional $3 million equity investment 3Plethora and Paul Capital Healthcare sign a $25 million revenue interest financing agreement with an option for an additional $3 million equity investment 4
... Derived Activator of Caspase/DIABLO Liquid. In ... AVOID FREEZE/THAW CYCLES. Amino acid residues 56 - ... His•Tag ® sequence was expressed in and ... released along with cytochrome c during ...
... Grace's medium was originally formulated to support ... Australian emperor gum moth,Antherea eucalypti. The medium ... more closely resembleAnthereahemolymph. The cell lines established ... first continuous lines developed. The basal medium, ...
...
... Fixed clamp trays and universal ... Magnetic drive Shaking ... 25 mm amplitude ... to 2000 ml, including Fernbach flasks ...
Biology Products: