Navigation Links
Monitoring and robust induction of nephrogenic intermediate mesoderm from human iPSCs
Date:1/25/2013

The research group led by Associate Professor Kenji Osafune and his colleague Shin-ichi Mae, both from Center for iPS Cell Research and Application (CiRA), Kyoto University in Japan, has succeeded in developing a highly efficient method of inducing human induced pluripotent stem (iPS) cells to differentiate into intermediate mesoderm, the precursor of kidney, gonad, and other cell lineages. This represents a major step toward realizing renal regeneration.

As nearly all kidney cells are derived through differentiation from intermediate mesoderm, to realize kidney regeneration requires first the development of an efficient technology for differentiating human iPS or embryonic stem (ES) cells into intermediate mesoderm.

The research team established a method through which fluorescent protein can be readily inserted into the human iPS/ES cell genome through homologous recombination and used it in human iPS cells to successfully introduce green fluorescent protein (GFP) into Odd-skipped related 1: (OSR1), a marker gene for intermediate mesoderm differentiation. This makes it possible to ascertain whether differentiation into the target intermediate mesoderm cells has been achieved.

The system was then used to establish a protocol for inducing iPS cell differentiation into intermediate mesoderm which produced a high success rate of 90% or more. It was confirmed that the resulting human intermediate mesoderm was able to differentiate into various types of kidney cell, and renal tubule structures were successfully generated.

The findings indicate the possibility of using iPS cells to create a supply of cells for use in renal regenerative medicine. The differentiation system developed by the researchers is also expected to provide a new research tool to help elucidate the developmental mechanism of intermediate mesoderm.

The next step required is to develop a technique that allows efficient and specific differentiation in
'/>"/>

Contact: CiRA International Public Communications Office
cira-pr@cira.kyoto-u.ac.jp
81-753-667-000
Center for iPS Cell Research and Application - Kyoto University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Chemical pollution in Europes seas: The monitoring must catch up with the science
2. New CU-NOAA monitoring system clarifies murky atmospheric questions
3. Tattoo-like devices for wireless pregnancy monitoring
4. Preventice Expands Options for Wireless Patient Monitoring with Qualcomm Life
5. NTU start-up launches worlds first 3-in-1 water monitoring system
6. Forest carbon monitoring breakthrough in Colombia
7. Ecological monitoring on bird populations in Europe re-evaluated
8. Sandia shows monitoring brain activity during study can help predict test performance
9. Detection, analysis of cell dust may allow diagnosis, monitoring of brain cancer
10. Call for global monitoring of infectious diseases in dogs and cats
11. Wildlife monitoring cameras click jaguar and ocelot photos
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/29/2014)... York City on September 19, 2014, leading geneticist and ... of Genome Sciences and Medicine at the University of ... of Tel Aviv University, was awarded the 2014 Lasker-Koshland ... to her honorary degree from TAU, Prof. King was ... Medicine, and for the past 18 years she has ...
(Date:10/29/2014)... Cambridge, MA and Hebei, China — BGI Tech and ... sequencing of the Jujube genome. Jujube is the most ... Jujube genome is particularly difficult to sequence due the ... is the first time that a genome in the ... been recently published in Nature Communications ...
(Date:10/28/2014)... One of the tragic realities of cancer is ... highly toxic and their effectiveness varies unpredictably from ... is poised to change this reality by rapidly ... on an individual,s cancer before chemotherapy begins. , ... headed by Assistant Professor Melissa Skala has developed ...
Breaking Biology News(10 mins):Geneticist and humanitarian: Prof. Mary-Claire King receives Lasker Award 2BGI Tech and Hebei Agricultural University complete the genome sequencing of the jujube tree 2Improving breast cancer chemo by testing patient's tumors in a dish 2Improving breast cancer chemo by testing patient's tumors in a dish 3
... of the embryo in the uterus have found a ... vessels that sustain the embryo. Without this protein, which is ... embryo is unlikely to survive. This is the first ... estrogen spurs cell differentiation and blood-vessel growth in the uterus ...
... techniques are increasing hope of restoring fertility for women ... destruction of reproductive tissue. The same techniques can also ... protect against extinction of endangered animal species by maintaining ... can used to produce offspring at some point in ...
... percent of fish species in North American streams, rivers ... most detailed evaluation of the conservation status of freshwater ... 700 fishes now listed represent a staggering 92 percent ... previous 1989 study published by the American Fisheries Society. ...
Cached Biology News:Gap junction protein vital to successful pregnancy, researchers find 2Cryopreservation techniques bring hopes for women cancer victims and endangered species 2Silent streams? Escalating endangerment for North American freshwater fish 2Silent streams? Escalating endangerment for North American freshwater fish 3
(Date:10/27/2014)... 27, 2014 Ryan Carfley , Managing ... to sit down with Bryan Hamilton, the publisher of the ... discuss talent in the Research Triangle region of North Carolina. ... highlighted at the roundtable include the role of social ... of work/life balance and the unique role that Millennials play ...
(Date:10/27/2014)... -- The Italian company Roadrunnerfoot has ... its artificial "lower limb", after the German giant ...      (Photo: http://photos.prnewswire.com/prnh/20141027/713097 ) , ... judgement has arrived: Roadrunnerfoot, the small Italian company ... prostheses with composite materials, has won against global ...
(Date:10/27/2014)... , Oct. 27, 2014 Kalorama Information says ... Ebola concern in the United States ... The healthcare market researcher said the FDA,s Emergency Use Authorization ... diagnostic tests for the detection of the Ebola virus ... useful.  Kalorama routinely assess the IVD industry and its ...
(Date:10/27/2014)... MA (PRWEB) October 27, 2014 ... profit. SoundConnect , an industry leading unified ... its partners and agents to deliver cloud-based audio ... accelerate growth opportunities. , With SoundConnect’s Q4 ... every twenty-five video and/or web conferencing licenses sold, ...
Breaking Biology Technology:Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 2Personify's Ryan Carfley Talks Recruiting with Triangle Business Journal, RPG Solutions 3Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2Kalorama: PCR The Go-to Test in Ebola Fight 2Kalorama: PCR The Go-to Test in Ebola Fight 3SoundConnect Unveils Q4 Partner Incentives 2
... 2011 NewCardio, Inc., (OTC BB: NWCI) a cardiovascular ... Clinilabs, will utilize QTinno®, NewCardio,s automated cardiac safety solution, ... new drug under development for a central nervous system ... one important aspect of the trial. Clinilabs, ...
... a biotechnology company developing peptide therapeutics for metabolic diseases, ... as Chief Executive Officer. Dr. Gottesdiener joins Rhythm from ... President and Late-Stage Therapeutic Group Leader, with responsibility for ... and previously as head of early-stage clinical development programs. ...
... -- an open-source, web-based platform for data-intensive biomedical ... a "cloud computing" resource. A team of researchers ... and molecular biology at Penn State University; Kateryna ... State; and James Taylor from Emory University, developed ...
Cached Biology Technology:Clinilabs will Conduct Phase 1 Trial Using QTinno® 2Clinilabs will Conduct Phase 1 Trial Using QTinno® 3Clinilabs will Conduct Phase 1 Trial Using QTinno® 4Clinilabs will Conduct Phase 1 Trial Using QTinno® 5Rhythm Names Keith M. Gottesdiener Chief Executive Officer 2Galaxy DNA-analysis software is now available 'in the cloud' 2Galaxy DNA-analysis software is now available 'in the cloud' 3