Navigation Links
Mimicking nature at the nanoscale: Selective transport across a biomimetic nanopore
Date:6/20/2011

Researchers at Delft University of Technology and the University of Basel have established a biomimetic nanopore that provides a unique test and measurement platform for the way that proteins move into a cell's nucleus. In the journal Nature Nanotechnology (June 19 - online), they report an artificial nanopore that is functionalized with key proteins which mimicks the natural nuclear pore. Upon testing the transport of individual proteins through the biomimetic pore, they found that most proteins cannot move through, but some specific ones can indeed pass. This is the hallmark of the intriguing selectivity that is also found in natural pores. The biomimetic pore is fully functional and can be used as a testing platform for studies of drug delivery into a cell's nucleus.

The nuclear pore complex

"Human cells have a nucleus, and proteins and RNA need to get in and out. This is regulated by small holes, called nuclear pore complexes. These are essential biological pores that act as gatekeepers of the cell nucleus. They transport proteins and RNA in and out of the nucleus in a highly selective manner, which means that some go through but others are blocked from passing. There is much debate on how this intriguing selectivity is achieved. Given the fact that it is very difficult to perform high-resolution measurements in the complex environment of the living cell, the exact mechanism is hard to resolve." Professor Cees Dekker, director of the Kavli Institute of Nanoscience at Delft and leader of this research, explains. In the new research by Dekker's group in collaboration with the group of dr. Roderick Lim of the University of Basel, they were able to make a biomimetic nanopore a synthetic pore that imitates the nuclear pore which acts as a new, powerful platform to monitor transport of individual proteins across.

Biomimetic nanopore

Dekker: "One promising approach to study this nuclear transport is biomimetics the development of synthetic systems that imitate biological structures and processes. Advances in nanotechnology now make it possible to study and shape matter at the nanometer scale, opening the way to imitate biological structures at the molecular level to both study and harness their ingenuity." The group of dr. Roderick Lim at the University of Basel purified the nuclear pore proteins and Dekkers group made the biomimetic nanopores of these by attaching these proteins to small holes in a solid-state support.

Selectivity

The new research, performed chiefly by lead author Stefan Kowalczyk, a graduate student in Dekkers lab, demonstrates that it is possible to establish a biomimetic nuclear pore and to monitor transport of individual proteins across the pore. Importantly, the biomimetic pore exhibits strong selectivity, just like the natural nuclear pore complex: ImpB proteins do pass the pores, whereas BSA proteins do not (as illustrated by the attached image). A differing degree of selectivity was found, depending on which exact nuclear pore proteins were used to functionalize the pore. The researchers have shown that the biomimetic pore is fully functional and can be used as a testing platform for studies of drug delivery into a cell's nucleus.


'/>"/>

Contact: Prof. Cees Dekker
c.dekker@tudelft.nl
Delft University of Technology
Source:Eurekalert

Related biology news :

1. Mimicking nature, water-based artificial leaf produces electricity
2. Boston Univ., Veterans Affairs find sports brain trauma may cause disease mimicking ALS
3. Wildlife Conservation Society finds wild cat mimicking monkey calls
4. Some vocal-mimicking animals, particularly parrots, can move to a musical beat
5. GigaBlitz will turn high-resolution images of nature into global inventory of organisms, habitats
6. Nanotechnologists must take lessons from nature
7. How natures best ideas inspire innovative new technologies
8. Nature helps to solve a sticky problem
9. 2011 PerkinElmer Signature Genomic Laboratories Travel Award winner announced
10. Nature paper calls for carbon labeling
11. New data published in Nature Genetics demonstrate that tiny LNA-based compounds developed by Santaris Pharma A/S inhibit entire disease-associated microRNA families
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/26/2016)... DUBLIN , April 27, 2016 ... of the  "Global Multi-modal Biometrics Market 2016-2020"  report ... ) , The analysts forecast ... a CAGR of 15.49% during the period 2016-2020.  ... a number of sectors such as the healthcare, ...
(Date:4/14/2016)... 2016 BioCatch ™, the ... announced the appointment of Eyal Goldwerger as ... Goldwerger,s leadership appointment comes at a time of ... deployment of its platform at several of the world,s ... discerns unique cognitive and physiological factors, is a winner ...
(Date:3/29/2016)... 2016 LegacyXChange, Inc. (OTC: ... SelectaDNA/CSI Protect are pleased to announce our successful effort ... variety of writing instruments, ensuring athletes signatures against counterfeiting ... from athletes on LegacyXChange will be assured of ongoing ... Bill Bollander , CEO states, "By ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... June 24, 2016 Epic Sciences unveiled ... cancers susceptible to PARP inhibitors by targeting homologous ... (CTCs). The new test has already been incorporated ... multiple cancer types. Over 230 clinical ... response pathways, including PARP, ATM, ATR, DNA-PK and ...
(Date:6/23/2016)... ... June 23, 2016 , ... UAS LifeSciences, one ... of their brand, UP4™ Probiotics, into Target stores nationwide. The company, which has ... add Target to its list of well-respected retailers. This list includes such fine ...
(Date:6/23/2016)... 2016 Houston Methodist Willowbrook Hospital has ... Association to serve as their official health care ... Willowbrook will provide sponsorship support, athletic training services, ... coaches, volunteers, athletes and families. "We ... Association and to bring Houston Methodist quality services ...
(Date:6/23/2016)... FRANCISCO , June 23, 2016   EpiBiome ... has secured $1 million in debt financing from Silicon ... ramp up automation and to advance its drug development ... its new facility. "SVB has been an ... beyond the services a traditional bank would provide," said ...
Breaking Biology Technology: