Navigation Links
MIT probe may help untangle cells' signaling pathways
Date:6/27/2008

CAMBRIDGE, Mass.--MIT researchers have designed a new type of probe that can image thousands of interactions between proteins inside a living cell, giving them a tool to untangle the web of signaling pathways that control most of a cell's activities.

"We can use this to identify new protein partners or to characterize existing interactions. We can identify what signaling pathway the proteins are involved in and during which phase of the cell cycle the interaction occurs," said Alice Ting, the Pfizer-Laubach Career Development Assistant Professor of Chemistry and senior author of a paper describing the probe published online June 27 by the Journal of the American Chemical Society.

The new technique allows researchers to tag proteins with probes that link together like puzzle pieces if the proteins interact inside a cell. The probes are derived from an enzyme and its peptide substrate. If the probe-linked proteins interact, the enzyme and substrate also interact, which can be easily detected.

To create the probes, the researchers used the enzyme biotin ligase and its target, a 12-amino-acid peptide.

Their work is conceptually related to an approach that uses GFPs (green fluorescent proteins), which glow when activated, as probes. Half of each GFP molecule is attached to the proteins of interest, and when the proteins interact, the GFP halves fuse and glow. However, this technique results in many false positives, because the GFP halves seek each other out and bind even when the proteins they are attached to are not interacting, said Ting.

The new probes could be used to study nearly any protein-protein interaction, Ting said. The researchers tested their probes on two signaling proteins involved in suppression of the immune system, and on two proteins that play a role in cell division. They are currently using the probe to image the interaction of proteins involved in synapse growth in live neurons.


'/>"/>

Contact: Elizabeth Thomson
thomson@mit.edu
617-258-5402
Massachusetts Institute of Technology
Source:Eurekalert

Page: 1

Related biology news :

1. Mini subs to probe odd structures in BC lake
2. UCLA researchers develop new PET scanning probe that will allowing monitoring of the immune system
3. Fast AFM probes measure multiple properties of biomolecules or materials simultaneously
4. ESF conference probes waters mysterious interactions at molecular level
5. NOAA aircraft to probe arctic pollution
6. Team probes mysteries of oceanic bacteria
7. Astronomy technology brings nanoparticle probes into sharper focus
8. Researchers probe a DNA repair enzyme
9. New MIT tool probes brain circuits
10. Studying component parts of living cells with carbon nanotube cellular probes
11. TAU scientists probe deep questions aboard EcoOceans environmental research ship
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/30/2014)... is available in German . ... prompt our fellow humans to behave socially? This is ... in game theory. Previous studies assumed that it is ... scientists from the Max Planck Institute for Evolutionary Biology ... an influence on the cooperative behaviour of others. However, ...
(Date:10/30/2014)... PA, October 30, 2014 – Bacteria in the ... critical for digestion. Yet, these same bacteria can ... if they penetrate the gut and enter the ... response to protect the body, chronic or systemic ... Prior research has established the involvement of inflammatory ...
(Date:10/30/2014)... New York | Heidelberg, 30 October 2014 Biological ... a better understanding of adsorption of solution ions ... biological cells. Now, a new study provides a ... and surrounding solution ions. Joanna Kotyńska and Zbigniew ... the authors of a study describing these findings, ...
Breaking Biology News(10 mins):Together we are strong -- or insufferable 2Together we are strong -- or insufferable 3Together we are strong -- or insufferable 4Breakdown in gut barriers to bacteria may promote inflammation and craving in alcoholics 2Ion adsorption matter in biology 2
... University of Melbourne shows how the malaria parasite ( ... sexual reproduction, a finding that could provide targets for ... parasite evades the human immune system. The work ... Dr Matthew Dixon and PhD student Megan Dearnley from ...
... of the Max Delbrck Center for Molecular Medicine (MDC) ... underlying the most common malformation of the brain in ... incompletely formed. Here a binding site (receptor) for cholesterol ... specific signals cannot be received, and the forebrain cannot ...
... like difficulty hearing or remembering things can complicate everyday ... to date has focused on the disease,s less frequent ... number of patients have expressed their desire for a ... attacks its own central nervous system, which can cause ...
Cached Biology News:Malaria parasite goes bananas before sex: New study 2MDC researchers reveal molecular mechanism underlying severe anomalies of the forebrain 2Wayne State University researcher's model will target causes of everyday MS symptoms 2Wayne State University researcher's model will target causes of everyday MS symptoms 3
(Date:10/30/2014)... James Sherley says he has been working ... cell technology since his days as a principal investigator at ... late 1990’s. Sherley founded the ASCTC as a new ... the intellectual property developed in Sherley’s research over the years ... of Technology (MIT), and more recently as a senior scientist ...
(Date:10/30/2014)... UAE (PRWEB) October 30, 2014 Grace Century ... Dr. James A. Manganello, Chairman of the Board, to the ... Bahamas. The appointment was made by the Ministry of ... September 24th, 2014. , More than a year ... Research Bill , Prime Minister Perry Christie said the government ...
(Date:10/30/2014)... one of the key challenges in the development of ... physicists. , In a paper published today (28 October) ... colleagues show how to make a new type of ... devices, such as high-precision sensors and specialised superfast computers, ... But the methods for trapping these tiny particles are ...
(Date:10/27/2014)... 2014 The Italian company ... and sell its artificial "lower limb", after the ...      (Photo: http://photos.prnewswire.com/prnh/20141027/713097 ) ... the definitive judgement has arrived: Roadrunnerfoot, the small ... accessible hi-tech prostheses with composite materials, has won ...
Breaking Biology Technology:Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 2Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 3Boston’s Adult Stem Cell Technology Center, LLC Introduces A New Technology for Monitoring Previously Elusive Adult Stem Cells 4Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2
... SHENZHEN, China, Aug. 15 /Xinhua-PRNewswire-FirstCall/ -- Mindray,Medical International ... manufacturer,and marketer of medical devices worldwide announced today ... Healthcare Unplugged Conference in,New York, NY on September ... be attended by Mr. Hang Xu, Mindray,s chairman ...
... ROCKVILLE, Md., Aug. 14 Neuralstem Inc.,(Amex: CUR ... ended,June 30, 2008., (Logo: http://www.newscom.com/cgi-bin/prnh/20061221/DCTH007LOGO ), ... reported a net loss of,$2,957,672, or $(0.09) per share, ... share, for the comparable 2007 period. Net loss attributable ...
... Totals $17.8 million at June 30 ... for September 5, NAPA, Calif., Aug. 14 Senetek ... the development,of technologies that target the science of healthy aging, ... Revenue for the quarter ended June 30, 2008 was ...
Cached Biology Technology:Mindray Medical to Attend Morgan Stanley Global Healthcare Unplugged Conference 2Neuralstem Reports Second Quarter Financial Results and Highlights 2Neuralstem Reports Second Quarter Financial Results and Highlights 3Neuralstem Reports Second Quarter Financial Results and Highlights 4Neuralstem Reports Second Quarter Financial Results and Highlights 5Neuralstem Reports Second Quarter Financial Results and Highlights 6Senetek PLC Reports Second Quarter 2008 Financial Results 2Senetek PLC Reports Second Quarter 2008 Financial Results 3
... assays utilize the membrane-permeant ester forms ... charged fluorescent beta-lactamase substrates, CCF2 and ... the cell, where cleavage by endogeneous ... their negatively charged forms, thereby trapping ...
... utilize the membrane-permeant ester forms (CCF2-AM ... fluorescent beta-lactamase substrates, CCF2 and CCF4. ... cell, where cleavage by endogeneous cytoplasmic ... negatively charged forms, thereby trapping them ...
1M stock solution (238mg/ml)...
Request Info...
Biology Products: