Navigation Links
MGH researchers describe new way to identify, evolve novel enzymes
Date:8/15/2007

The intricate interplay of proteins and other chemicals that underlies most biological activities requires the participation of enzymes, specialized molecules that accelerate chemical reactions between molecules. The creation of totally new enzymes can help improve the synthesis of chemicals and pharmaceuticals, devise new tools for molecular biology research, and develop new therapies. In the August 16 issue of Nature, two Massachusetts General Hospital (MGH) researchers describe a way of creating novel enzymes that, for the first time, does not require prior understanding of exactly how the enzymes work.

To date, the only source of enzymes has been biology, says Jack Szostak, PhD, of the MGH Department of Molecular Biology, the reports senior author. Great efforts are going into modifying and improving these natural enzymes, and our work demonstrates the potential of evolving completely new enzymes in the laboratory.

Szostak and his co-author Burckhard Seelig, PhD, used a technique called mRNA display previously developed in Szostaks lab that allows the identification and amplification of proteins that fit particular criteria. In order to create an enzyme that would stimulate or catalyze the joining of two segments of RNA in a way that does not occur naturally, they began by generating a library of 4 trillion small proteins with slight variations in their sequences. Each protein was then brought together with the RNA segments to be joined, called substrates.

If a particular protein induced the RNA substrates to join, resulting in a significantly larger molecule, that signified the protein was an active enzyme. The investigators could select out the larger RNA strands, generate more of the enzymes, and repeat the experiment. The induction of random mutations to produce different forms of the enzymes and reducing the time allowed for the splicing reaction enabled the development of more efficient versions by means of guided
'/>"/>

Contact: Sue McGreevey
smcgreevey@partners.org
617-724-2764
Massachusetts General Hospital
Source:Eurekalert

Page: 1 2

Related biology news :

1. Researchers discover way to make cells in the eye sensitive to light
2. Researchers find how protein allows insects to detect and respond to pheromones
3. Researchers Uncover Key Step In Manufacture of Memory Protein
4. NYU researchers simulate molecular biological clock
5. Researchers reveal the infectious impact of salmon farms on wild salmon
6. Researchers identify target for cancer drugs
7. Vital step in cellular migration described by UCSD medical researchers
8. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
9. UCSD researchers maintain stem cells without contaminated animal feeder layers
10. Researchers discover molecule that causes secondary stroke
11. Researchers find missing genes of ancient organism
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/31/2014)... developed a new test that can distinguish between birds ... avian influenza virus or "bird flu" with those that ... step in the fight against this often fatal strain ... populations of South East Asia, particularly Indonesia and Vietnam. ... transmission to humans and the threat of a pandemic ...
(Date:10/31/2014)... 30, 2014  HITLAB SM , a healthcare ... its inaugural HITLAB Innovators Summit SM on ... HITLAB will partner with the Clinton Foundation,s Health ... ProtoHack, Columbia Business School Alumni Club of ... Engineering and Operations Research, bringing together leaders in ...
(Date:10/30/2014)... has received a $1.04 million grant from the ... and reduce osteoarthritis using a patient,s own stem ... of biodegradable polymers., Liping Tang, a bioengineering professor ... the research is primarily focused on helping soldiers ... found that if we inject microscaffolding that we,ve ...
Breaking Biology News(10 mins):New step towards eradication of H5N1 bird flu 2HITLAB Announces Inaugural HITLAB Innovators Summit 2HITLAB Announces Inaugural HITLAB Innovators Summit 3UTA researcher uses microscaffolding injections to mend cartilage, prevent osteoarthritis 2
... like cancer typically destroy nasty malignant cells, while ... in synthetic biology, researchers are designing molecules intelligent ... cells alone. "We basically design molecules that actually ... of the cellular state before delivering the therapeutic ...
... 1996 marked the beginning of a revolution in biological and ... by now the number of characterised species is approaching 1000, ... a few years away mapping the genetic evolution of ... able to identify the genetic causes of human disease and ...
... , A mutual epidemiological relationship between aggressive periodontitis and ... at the universities of Kiel, Dresden, Amsterdam and Bonn have ... on chromosome 9, which maps to a genetic region that ... latest edition of the specialist journal PLoS Genetics . ...
Cached Biology News:Engineers create intelligent molecules that seek-and-destroy diseased cells 2Engineers create intelligent molecules that seek-and-destroy diseased cells 3Genetic differences between yeasts greater than those between humans and chimpanzees 2
(Date:10/31/2014)... Caustic soda is utilized in the production of zeolites, sodium ... lead in the global caustic soda market, followed by the ... in the USA, Germany, Brazil and Canada, is currently the ... of output and production capacities. , The global caustic soda ... year in the years ahead. The APAC region is the ...
(Date:10/31/2014)... Lawrence, MA (PRWEB) October 30, 2014 ... from Latin America were hosted by Charm Sciences ... October. During their week in the US, the ... chemical residue control, hygiene management, and process verification, ... state-of-the-art facility. , This was the first visit ...
(Date:10/31/2014)... (PRWEB) October 31, 2014 Following the ... a free learning webinar to introduce its new product ... directors and biostatisticians. , Launched in September, Formedix On ... suite of clinical trial automation tools. For the first ... web browser. And by introducing Formations – a revolutionary ...
(Date:10/31/2014)... The report "Dairy Alternative (Beverage) ... Flavored, Sweetened, Unsweetened), Channel (Supermarket, Health Store, Pharmacy, ... Forecast to 2018," defines and segments the dairy ... of revenue and volume for plant-based dairy alternative ... reach $14 Billion by 2018 with the Asia-Pacific ...
Breaking Biology Technology:China to Dominate Caustic Soda Market Through 2018, Says Merchant Research & Consulting in Its New Study 2Charm Sciences Hosts Executives from Latin America for Dairy Safety Executive Seminar 2Formedix to Introduce "Revolutionary" Clinical Trial Automation Software with Learning Webinar 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 2Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 3Dairy Alternative Beverage Market Projected to Reach $14 Billion by 2018 - Report by MarketsandMarkets 4
... are pleased to release the third in a ... , This third Podcast titled "Commercialization ... crucial issues around how nanotechnology can be taken ... commercial outcomes. , Dr Peter Binks, CEO of ...
... 7 Dendreon Corporation (Nasdaq:,DNDN) today announced that ... position of senior vice president of clinical affairs ... with over a decade of oncology,drug development and ... was instrumental to the filing of the company,s ...
... in the Department of Physics & Engineering ... with colleagues from the University of California, ... authored the article, High-frequency single-photon source with ... December 2007 issue of Nature Photonics ( ...
Cached Biology Technology:Commercialization of nanotechnology 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 2Dendreon Promotes Dr. Mark Frohlich to Senior Vice President of Clinical Affairs and Chief Medical Officer 3Professor Strauf's research is Nature Photonics’ cover article 2
... Ambion's custom siRNA service provides the premium ... silencing research. Custom siRNAs can be readily ... base target sequence, or by providing the ... out Ambion's Silencer Validated and Silencer Pre-designed ...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
, Premium quality synthesis and purification , You provide the sequence, we'll do the rest , Rigorous quality control , Your choice of purification method, amount and format , Available annealed...
Human TGF-beta RIIb Affinity Purified Polyclonal Ab...
Biology Products: