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Iowa State, USDA researchers discover eye test for neurological diseases in livestock
Date:10/19/2010

AMES, Iowa The eyes of sheep infected with scrapie a neurological disorder similar to mad cow disease return an intense, almost-white glow when they're hit with blue excitation light, according to a research project led by Iowa State University's Jacob Petrich.

The findings suggest technologies and techniques can be developed to quickly and noninvasively test for transmissible spongiform encephalopathies, progressive and fatal neurological diseases such as mad cow disease in cattle and Creutzfeldt-Jakob disease in humans. Petrich, in fact, is working to develop a testing device.

The findings were published earlier this year in the journal Analytical Chemistry. The project was supported by a grant from the U.S. Department of Defense.

The research is the result of an accidental discovery while Petrich and his collaborators were developing a fluorescence spectroscopy device that's now used in slaughterhouses to test livestock carcasses for feces and possible E. coli contamination.

"One day we were testing the apparatus by shining light on the carcass and we saw the spinal cord glow it fluoresced," said Petrich, professor and chair of Iowa State's chemistry department. "We saw the spinal cord through the skin. The light was pretty intense. It was an amazing result."

That sparked some new thinking: Maybe fluorescence technology could be used to test animals for transmissible spongiform encephalopathies such as bovine spongiform encephalopathy what's often called mad cow disease. To reduce the risk of human exposure to the diseases, the brains and spinal cords of animals are removed during slaughter and processing. But there is no quick test to identify animals with the diseases.

And so Petrich and a team of researchers began studying the feasibility of a fluorescence test. The team included Ramkrishna Adhikary, an Iowa State graduate student in chemistry; Prasun Mukherjee, a former Iowa State graduat
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Contact: Jacob Petrich
jwp@iastate.edu
515-294-9422
Iowa State University
Source:Eurekalert

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