Navigation Links
In subglacial lake, surprising life goes on
Date:7/5/2013

ng culturing colonies of bacteria and fungi, but the process was slow, especially for graduate students who needed results for their theses.

"We started thinking of doing it a different way," Rogers said.

Instead of culturing living organisms, they concentrated on sequencing DNA and RNA in the ice. These methods, called metagenomics and metatranscriptomics, produced thousands of sequences at a time that were then analyzed using computers procedures referred to collectively as "Big Data" methods. In contrast, it usually took years to generate enough cultured organisms for a few dozen sequences.

The problem changed from having too few sequences to having too many sequences to analyze, Rogers said. After two years of computer analysis, the final results showed that Lake Vostok contains a diverse set of microbes, as well as some multicellular organisms.

Long before he began using metagenomics and metatranscriptomics to study the ice, Rogers and his team had developed a method to ensure purity. Sections of core ice were immersed in a sodium hypochlorite (bleach) solution, then rinsed three times with sterile water, removing an outer layer. Under strict sterile conditions, the remaining core ice was then melted, filtered and refrozen.

"Using this method, we can assure its reliability almost to 100 percent," Rogers said.

Eventually, the process rendered pellets of nucleic acids containing both DNA and RNA, able to be sequenced.

Rogers said the team erred strongly on the conservative side in reporting its results, including only those sequences of which it could be absolutely certain were from the accretion ice, but there are a multitude of others he feels are probably from the lake, opening the door to additional investigation.

The DNA sequences they produced have been deposited in the National Center for Biotechnology GenBank database, and will be available to other researchers for further study
'/>"/>

Contact: Jen Sobolewski
jsobole@bgsu.edu
Bowling Green State University
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. Genetic survey of endangered Antarctic blue whales shows surprising diversity
2. Surprising pine beetle breeding habits help explain increasing tree damage, says CU study
3. A surprising new kind of proton transfer
4. First-of-its-kind study reveals surprising ecological effects of earthquake and tsunami
5. Seabirds study shows plastic pollution reaching surprising levels off coast of Pacific Northwest
6. Nuclear weapons surprising contribution to climate science
7. Surprising demographic shifts in endangered monkey population challenge conservation expectations
8. Despite their thick skins, alligators and crocodiles are surprisingly touchy
9. Surprising teaching tool in K-12 science education -- Zebrafish research
10. Climate changes effects on temperate rain forests surprisingly complex
11. New look at cell membrane reveals surprising organization
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/20/2014)... demonstrate a bizarre property never before seen at a ... scientists at the University of Cambridge. The property ... application as wide-ranging as soundproofing, super-absorbent sponges and bulletproof ... if one pulls on an elastic band, the elastic ... squeeze a material and it will expand for ...
(Date:4/20/2014)... used to treat lung, breast and pancreatic cancers also ... the University of California, San Diego School of Medicine ... the surface of drug-resistant tumors that appears responsible for ... of cancer cells. , The findings, published in ... Cell Biology , may point to new therapeutic opportunities ...
(Date:4/20/2014)... with Down syndrome have a heightened risk of developing ... able to explain why. Now, a team of ... the two conditions. , In a study posted online ... researchers track the genetic chain of events that links ... havoc that occurs in ALL. Their findings are ...
Breaking Biology News(10 mins):Bulletproof nuclei? Stem cells exhibit unusual absorption property 2Cancer stem cells linked to drug resistance 2Dana-Farber researchers uncover link between Down syndrome and leukemia 2Dana-Farber researchers uncover link between Down syndrome and leukemia 3
... published by ASM Press educates the reader by focusing ... teaching virology for more than 35 years, Virology: Molecular ... understanding of fundamental virology by emphasizing principles and discussing ... Molecular Biology and Pathogenesis is meant to be used ...
... have found that the hydrodynamic environment of fish can shape ... the Journal of Experimental Biology Web site, was ... Dynamics. Catch a glimpse of a fish,s body shape, ... and mackerel look as if they should outpace frilly reef ...
... Mediterranean Sea has a stable and constant dolphin population off ... also consider the dolphins," says Dr. Aviad Scheinin of the ... commercial fishing endangers dolphin populations in the Mediterranean. This has ... University of Haifa,s Department of Maritime Civilizations. "Unfortunately, we turn ...
Cached Biology News:Virology text focuses on families 2Research finds water movements can shape fish evolution 2Commercial fishing endangers dolphin populations 2Commercial fishing endangers dolphin populations 3
(Date:1/15/2014)... VA (PRWEB) January 15, 2014 Two ... have continued their sponsorship of an annual competition for ... in, and access to, innovative STEM study. The competition ... TEAMS: Tests of Engineering Aptitude, Mathematics, and Sciences ...
(Date:1/15/2014)... (PRWEB) January 15, 2014 Freeslate, ... solutions, today announced that Lupin Limited, one of India’s ... CM Protégé PharmD System for high throughput ... India, is focused on a wide range of quality, ...
(Date:1/14/2014)... DC (PRWEB) January 14, 2014 Carahsoft ... for Thursday, January 23, 2014 at 2pm EST (11am ... Knowledge.” The topic focuses on how technology can turn ... critical decisions for government agencies. The online webinar will ...
(Date:1/14/2014)... EquitiesIQ, a leading informational research provider, ... is an emerging biomedical company acquiring, developing, manufacturing, and ... , Free report download: http://equitiesiq.com/reports/alliqua/ , In ... team and Board, which launched the company’s new strategy ...
Breaking Biology Technology:Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 2Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 3Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 4Technology Student Association Partners with Leaders in STEM Education Advocacy for Annual Competition 5Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... YORK , July 8 Bion Environmental Technologies, ... a public issues forum on July 14 , hosted ... Relations at their Campus Center auditorium.  The forum, sought by ... development group, will address Bion,s proposed beef cattle production facility ...
... Calif. , July 8 ... announced the completion of a Series A financing totaling ... with U.S. Venture Partners, Advanced Technology Ventures, Delphi Ventures ...  The capital will be used to support the company,s ...
... ... interactive healthcare marketing at Digital Pharma West. , ... (PRWEB) -- Viscira™, a leading provider of interactive and ... Return on Interactive” at the Digital Pharma West (DPW) conference last week in San ...
Cached Biology Technology:Bion Confirms Participation in SUNY Oswego's Forum 2Bion Confirms Participation in SUNY Oswego's Forum 3Bion Confirms Participation in SUNY Oswego's Forum 4Calithera Biosciences Closes $40 Million in Series A Financing 2Calithera Biosciences Closes $40 Million in Series A Financing 3Calithera Biosciences Closes $40 Million in Series A Financing 4Viscira Delivers Key Presentation to Pharmaceutical Marketing Professionals at Leading Digital Industry Conference 2
Homo sapiens similar to Cytosolic acyl coenzyme A thioester hydrolase Antigen: Peptide...
Borrelia Burgdorfen (IgG) -Ab EIA Sample Size: 100 l...
96 Well Base Plate...
... ANTI-RNase is a protein ribonuclease inhibitor that noncovalently ... No inhibition is seen with RNase 1, T1, ... distinct from human placental ribonuclease inhibitor in that ... and does not release active RNase in the ...
Biology Products: