Navigation Links
In subglacial lake, surprising life goes on
Date:7/5/2013

ng culturing colonies of bacteria and fungi, but the process was slow, especially for graduate students who needed results for their theses.

"We started thinking of doing it a different way," Rogers said.

Instead of culturing living organisms, they concentrated on sequencing DNA and RNA in the ice. These methods, called metagenomics and metatranscriptomics, produced thousands of sequences at a time that were then analyzed using computers procedures referred to collectively as "Big Data" methods. In contrast, it usually took years to generate enough cultured organisms for a few dozen sequences.

The problem changed from having too few sequences to having too many sequences to analyze, Rogers said. After two years of computer analysis, the final results showed that Lake Vostok contains a diverse set of microbes, as well as some multicellular organisms.

Long before he began using metagenomics and metatranscriptomics to study the ice, Rogers and his team had developed a method to ensure purity. Sections of core ice were immersed in a sodium hypochlorite (bleach) solution, then rinsed three times with sterile water, removing an outer layer. Under strict sterile conditions, the remaining core ice was then melted, filtered and refrozen.

"Using this method, we can assure its reliability almost to 100 percent," Rogers said.

Eventually, the process rendered pellets of nucleic acids containing both DNA and RNA, able to be sequenced.

Rogers said the team erred strongly on the conservative side in reporting its results, including only those sequences of which it could be absolutely certain were from the accretion ice, but there are a multitude of others he feels are probably from the lake, opening the door to additional investigation.

The DNA sequences they produced have been deposited in the National Center for Biotechnology GenBank database, and will be available to other researchers for further study
'/>"/>

Contact: Jen Sobolewski
jsobole@bgsu.edu
Bowling Green State University
Source:Eurekalert

Page: 1 2 3 4 5

Related biology news :

1. Genetic survey of endangered Antarctic blue whales shows surprising diversity
2. Surprising pine beetle breeding habits help explain increasing tree damage, says CU study
3. A surprising new kind of proton transfer
4. First-of-its-kind study reveals surprising ecological effects of earthquake and tsunami
5. Seabirds study shows plastic pollution reaching surprising levels off coast of Pacific Northwest
6. Nuclear weapons surprising contribution to climate science
7. Surprising demographic shifts in endangered monkey population challenge conservation expectations
8. Despite their thick skins, alligators and crocodiles are surprisingly touchy
9. Surprising teaching tool in K-12 science education -- Zebrafish research
10. Climate changes effects on temperate rain forests surprisingly complex
11. New look at cell membrane reveals surprising organization
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/22/2014)... of Standards and Technology (NIST), working with the ... for analyzing biological cells and tissues based on ... is an advanced form of the widely used ... that are 10,000 times stronger than obtained from ... obtained from comparable "coherent Raman" instruments, and uses ...
(Date:7/22/2014)... as disease causing agents, some viruses can perform ... shape the world we live in today, according ... of Microbiology. , "Viruses participate in essential Earth ... planet, from contributing to biogeochemical cycles, shaping the ... Marilyn Roossinck of Pennsylvania State University, a member ...
(Date:7/22/2014)... Stem cells offer much promise for treating damaged organs ... survival is poor, limiting their effectiveness. New methods are ... optimize their therapeutic function after transplantation, as described in ... a peer-reviewed journal from Mary Ann Liebert, Inc., publishers. ... Open Access website . , In ...
Breaking Biology News(10 mins):Enhanced NIST instrument enables high-speed chemical imaging of tissues 2Enhanced NIST instrument enables high-speed chemical imaging of tissues 3Report on viruses looks beyond disease 2
... key to the blinding effects of diabetic retinopathy is ... Georgia researchers said. Interleukin-6, known to contribute ... helps ignite inflammation of the retina, a first step ... blindness is working-age adults, MCG researchers reported online in ...
... of dollars each year on exotic smelling perfumes and lotions ... a new study in Psychological Science , a journal ... be the best way to capture a potential mate,s attention. ... habits: Animal studies have shown that male testosterone levels are ...
... Malaga have created the ,Inventory of Daily Stressors, a ... physical appearance, taking part in numerous extracurricular activities and ... that increase the risk of suffering from childhood stress. ... to assess daily stress amongst schoolchildren", Mara Victoria Trianes, ...
Cached Biology News:Discovery points toward anti-inflammation treatment for blinding disease 2A new method to measure childhood stress 2
(Date:7/22/2014)... July 22, 2014 The DNA ... and concise visual representation of history, migration, and culture. ... always regaled by family history stories stemming from Europe. ... the "old country." Pictures and 8mm home movies painted ... of the family, and Steve ordered a test from ...
(Date:7/22/2014)... 2014  Neogen Corporation (NASDAQ: NEOG ) ... which ended May 31, increased 19% from the previous ... compared to the prior year,s $27,190,000. Adjusted for a ... share in the current year were $0.76, compared to ... for the 2014 fiscal year established new all-time highs ...
(Date:7/22/2014)... SHENZHEN, China , 22 juli 2014 ... van BGI, ,s wereld grootste organisatie op het gebied ... nieuwe service ten behoeve van hele menselijke genoomsequentie via ... Genomics. In 2013 acquireerde BGI Complete ... aanzien staat vanwege de in eigen beheer ontwikkelde sequentietechnologieën ...
(Date:7/22/2014)... 22, 2014 In chemical and ... increasingly common sight. The rapid rise of TDL ... well- established as a core gas measurement technology, ... made them the gas analyzer technology of choice ... encountered in some processes have limited their application ...
Breaking Biology Technology:DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 2DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 3DNA Test from DNA Spectrum Shows Heritage Like a Map of the Past 4Neogen reports 19% increase in annual revenues 2Neogen reports 19% increase in annual revenues 3Neogen reports 19% increase in annual revenues 4Neogen reports 19% increase in annual revenues 5Neogen reports 19% increase in annual revenues 6Neogen reports 19% increase in annual revenues 7Neogen reports 19% increase in annual revenues 8BGI Tech introduceert service op gebied van hele menselijke exoomsequencing op geavanceerd Complete Genomics-platform 2METTLER TOLEDO Shows How Tunable Diode Lasers (TDLs) Can Be Installed Where No TDLs Have Gone Before 2
... , SEATTLE, Aug. 5 ... Murray, M.D., Ph.D. as Vice President of Clinical Development. ... at Omeros, and currently is leading the ongoing clinical ... the Company,s lead candidate being evaluated in a Phase ...
... The FIME 2009 ... John Rizvi will deliver a presentation titled, "Preservation of Legal Rights in Medical Innovations" at ... ... 2009 at 1:00 PM, attorney John Rizvi will deliver a presentation titled, "Preservation of Legal ...
... to develop organic solar cells that can be produced easily ... to generate electricity. But a major obstacle is coaxing ... the nanoscale (tinier than 2-millionths of an inch) to be ... to develop cells made from low-cost plastics that will transform ...
Cached Biology Technology:Omeros Appoints New Vice President of Clinical Development 2Patent Attorney John Rizvi Speaks at FIME International Medical Expo 2Plastics that convert light to electricity could have a big impact 2Plastics that convert light to electricity could have a big impact 3