Navigation Links
Identifying microbial species
Date:7/3/2014

Millions of microbial species populate the world, but so far only a few have been identified due to the inability of most microbes to grow in the laboratory. Edgar Goluch, an engineer, and Slava Epstein, a biologist, aim to change this. The pair, both researchers at Northeastern University, has developed a device that allows scientists to cultivate a single species of bacteria that can then be studied and identified.

Goluch's previous research devices incorporated permeable membranes that allow sequestered bacteria to be exposed to the nutrients and molecules of their native environment. But natural competition between species, even in the wild, has so far limited the number of species of bacteria that biologists have been able to isolate with these methods and in traditional lab settings.

Goluch and Epstein's device, detailed in a paper released July 1 in the journal PLOS ONE, solves this problem. This new device permits just a single bacterial cell to enter an inner chamber containing a food source, to which the only access is a microscopic passageway just slightly narrower than a single cell. The passageway is so small that the first cell to enter it gets stuck, blocking entry by any other cell or species. Once inside, this cell pro-liferates as in previous devices, and when it does it fills up the inner chamber with a pure, single-species sample, since it is isolated from competition from other species.

In the paper, the team demonstrates the device's ability to separate mixtures of cell types in a laboratory setting. In one experiment, the researchers separated two different bacterial species whose cells are slightly different sizesE. coli and P. aueruginosa. In a second experiment, they isolated a combination of similarly sized but differently shaped species that commonly show up together in the marine environmentRoseobacter sp. and Pscyhoserpens sp. Finally, they used the device to separate cells of the same species that had been differentially tagged to glow either red or green. This final experiment validates the hypothesis that the cells grown inside the food chamber are daughters of the single cell caught in the entryway. Epstein will test the devices in the biological setting beginning this month during a research trip to Greenland.

Going forward, funding from an Instrument Development Biological Research Grant from the National Science Foundation will enable Goluch and his team of engineers to begin optimizing the device and its manufacture on a larger scale.


'/>"/>

Contact: John O'Neill
j.oneill@neu.edu
617-373-5460
Northeastern University
Source:Eurekalert  

Related biology news :

1. Beyond the microscope: Identifying specific cancers using molecular analysis
2. A new type of data papers designed to publish online interactive keys identifying biodiversity
3. Researchers develop new method for identifying lung nodules
4. UC research takes a new approach to identifying food deserts
5. Identifying a mystery channel crucial for hearing
6. DNA test better than standard screens in identifying fetal chromosome abnormalities
7. New technique for identifying gene-enhancers
8. Two researchers known for identifying and treating bubble boy disease honored by March of Dimes
9. Viral infections: Identifying the tell-tale patterns
10. Novel dark matter searches, identifying muzzle flashes, and more at 2014 APS DAMOP annual meeting
11. Childrens Research Institute finds key to identifying, enriching mesenchymal stem cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Identifying microbial species
(Date:4/26/2016)... DUBLIN , April 27, 2016 ... of the  "Global Multi-modal Biometrics Market 2016-2020"  report ... ) , The analysts forecast ... a CAGR of 15.49% during the period 2016-2020.  ... a number of sectors such as the healthcare, ...
(Date:4/15/2016)... , April 15, 2016 ... "Global Gait Biometrics Market 2016-2020,"  report to their ... ) , ,The global gait biometrics market ... 13.98% during the period 2016-2020. Gait ... which can be used to compute factors that ...
(Date:3/31/2016)... 31, 2016   ... the "Company") LegacyXChange is excited to release ... soon to be launched online site for trading 100% ... ) will also provide potential shareholders a sense of ... to an industry that is notorious for fraud. The ...
Breaking Biology News(10 mins):
(Date:5/23/2016)... ... May 23, 2016 , ... PrecisionAg® Media ... in 2017 and Beyond. The paper outlines the key trends that are creating ... , “We’ve witnessed a lot of highs and lows as the precision agriculture ...
(Date:5/23/2016)... May 23, 2016 - Leading CRO,s Use ... - Frontage Implement a Single Platform to Manage End-to-end Operations ... Within the Bioanalytical lab Frontage Laboratories, a full-service contract ... and China , has selected IDBS, ... In addition to serving as the global electronic lab notebook (ELN), ...
(Date:5/22/2016)... (PRWEB) , ... May 22, 2016 , ... Doctors in ... in combating the asbestos cancer, malignant mesothelioma. Surviving Mesothelioma has just posted an article ... in the University of Rome’s Department of Clinical Sciences and Translational Medicine evaluated more ...
(Date:5/20/2016)... ... ... Korean researchers say Manumycin A triggers apoptosis, or natural cell death, in ... disease. Surviving Mesothelioma has just posted an article on the new study. Click ... mesothelioma study on the fact the Manumycin A, a derivative of Streptomyces parvulus, is ...
Breaking Biology Technology: