Navigation Links
From scourge to saint: E. coli bacteria becomes a factory - to make cheaper, faster pharmaceuticals
Date:3/26/2012

Escherichia coli a bacteria considered the food safety bane of restaurateurs, grocers and consumers is a friend. Cornell University biomolecular engineers have learned to use E. coli to produce sugar-modified proteins for making pharmaceuticals cheaper and faster. (Nature Chemical Biology, March 25, 2012.)

Matthew DeLisa, Cornell associate professor of chemical and biomolecular engineering, and his research team, now have published a novel method for engineering human therapeutic glycoproteins simply and quickly by using E. coli bacteria as a platform. Their methods are now being developed and commercialized through a startup company, Glycobia Inc., which recently took up residence in Cornell's McGovern Family Center for Venture Development in the Life Sciences. While there are no firm plans yet, the professor hopes that within a year, testing this kind of pharmaceutical could be done at the Weill Cornell Medical College in Manhattan.

Glycoproteins are proteins that are modified at specific amino acid "acceptor" sites with complex carbohydrate structures, or oligosaccharides a basic human chemical reaction that's essential to life. That's why specifically designed, genetically engineered glycoproteins are so commonly used as drugs they bind to certain protein receptor sites and, for example, block cancer cells from multiplying. Among glycoproteins used to treat diseases today are monoclonal antibodies and interferons.

Current manufacturing methods rely mainly on costly, time-consuming mammalian culture cells, such as the Chinese Hamster Ovary (CHO) cell line. The process is also susceptible to viral contamination, further driving up production cost. In fact in 2009, another biopharmaceutical company temporarily shut down its plant after such a contamination occurred.

The Cornell research uses a method to assemble a synthetic pathway for the simple and quick production of a glycoprotein that forms the basis of many of today's therapeutic protein drugs, including, for example, the protein GCase, used in a drug that treats Gaucher's disease. To do so, they artificially introduced the machinery of glycosylation the chemical process by which proteins become glycoproteins into E. coli cells, rather than animal cells.

The synthetic pathway they designed, which can be tailored to many amino acid acceptor sites to make different drugs, starts with native enzymes in E. coli. Added to that was a mixture of four enzymes taken from yeast cells, which triggered the biosynthesis of a specific glycan (sugar structure) that resembles the core structure found in virtually all eukaryotic glycans. A fifth enzyme from the bacterium, Campylobacter jejuni, transferred these core glycans to pre-engineered protein acceptor sites, resulting in the desired glycoproteins.

DeLisa and his colleagues are now working to improve their approach that they call "glycans by design" using the enzyme-based protein production method to specifically tailor sugar structures to make many different glycans and glycoproteins.


'/>"/>
Contact: Blaine Friedlander
bpf2@cornell.edu
607-254-8093
Cornell University
Source:Eurekalert

Related biology news :

1. Seafarers scourge provides hope for biofuel future
2. Disinfectants can make bacteria resistant to treatment
3. H. Pylori bacteria may help prevent some esophageal cancers
4. Scientists discover bacteria that can cause bone infections
5. Waste from gut bacteria helps host control weight, UT Southwestern researchers report
6. Gene against bacterial attack unravelled
7. Predatory bacterial swarm uses rippling motion to reach prey
8. Bacteria manage perfume oil production from grass
9. Nature study demonstrates that bacterial clotting depends on clustering
10. Battling bacteria in the blood: Researchers tackle deadly infections
11. Shifts in soil bacterial populations linked to wetland restoration success
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/19/2019)... ... , ... Hosted at the Las Vegas Convention Center, and brought to you ... gathering of MJBiz business professionals in the world. With over 1,300 exhibitors from more ... no show quite like it. Atlantic Ultraviolet will exhibit at Booth C9217, showcasing its ...
(Date:11/12/2019)... ... 12, 2019 , ... In’Tech Medical SAS ( http://www.intech-medical.com ), ... of a Global Regulatory Affairs Department, to support Medical Device companies across its ... element to any Medtech organization is more important than Research & Development, Manufacturing ...
(Date:11/12/2019)... ... November 12, 2019 , ... ... proposals from nonprofit U.S. universities and research institutions for its Instrumentation Grant ... of up to $1.2 million per site for the acquisition of instrumentation, development ...
Breaking Biology News(10 mins):
(Date:11/9/2019)... ... November 08, 2019 , ... PathAI, ... research, today announced the results of analyses examining the severity of nonalcoholic ... 3 selonsertib studies (STELLAR). Strong correlations were demonstrated between AI-powered and manual ...
(Date:11/5/2019)... POWAY, Calif. (PRWEB) , ... November 05, 2019 , ... Ole, a German Shepherd, was ... difficulty with stairs and jumping. Though his energy level and his willingness to play ... to veterinary surgeon Dr. Holly Mullen of VCA Emergency Animal Hospital and Referral Center ...
(Date:10/30/2019)... ... October 30, 2019 , ... Western University of Health ... and technology play in educating the next generation of health care providers. , ... presentations by WesternU administrators and representatives of companies working with the University on ...
(Date:10/22/2019)... ... October 22, 2019 , ... nQ Medical, Inc. of Cambridge, MA, was ... Companies List which was announced at a showcase event yesterday in Los Angeles. nQ ... strong business plans, and impressive near-term growth projections to be named one of the ...
Breaking Biology Technology: