Navigation Links
Family trees for yeast cells
Date:5/13/2013

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families. The team of Dr. Aime Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the renowned scientific journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads (DOI: 10.1038/nmeth.2479).

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads". To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that un
'/>"/>

Contact: Sabine Mosch
sabine.mosch@uni.lu
352-466-644-6423
University of Luxembourg
Source:Eurekalert

Page: 1 2

Related biology news :

1. New research constructs ant family tree
2. Signalling pathways meeting targeting the HER/EGFR family: Focus on breast, lung and colorectal cancers
3. FASEB SRC announces conference: TGF-β Superfamily: Signaling in Development & Disease
4. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
5. Bats split on family living
6. Networking ability a family trait in monkeys
7. All in the family: A genetic link between epilepsy and migraine
8. Bloomberg School receives $28 million for family planning advocacy
9. CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs
10. Millipede family added to Australian fauna
11. Helping family is key for social birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/15/2014)...   Neurotechnology , a provider of high-precision ... the VeriLook Surveillance 3.0 software development kit ... face identification using live video streams from single ... version not only identifies faces in a crowd, ... they are moving through the video frame. It ...
(Date:10/14/2014)... house fly genome for the first time, revealing robust ... that thrives in pathogen-rich dung piles and garbage heaps. ... Genome Biology , will increase understanding of house ... adapt to resist insecticides, which could lead to novel ... and transmit more than 100 human and animal diseases, ...
(Date:10/14/2014)... LA JOLLA, CA – October 14, 2014 – Scientists ... $6.6 million from the National Institutes of Health (NIH) ... most prevalent virus-induced hemorrhagic fever disease in Africa. The ... disease and why some patients die, while others survive ... researchers to understand the basic mechanism of how Lassa ...
Breaking Biology News(10 mins):VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3House fly genome reveals expanded immune system 2Scripps Research Institute team receives $6.6 million to investigate deadly Lassa virus 2
... in the February 15th issue of Genes & Development ... in sperm while they reside in the female reproductive ... of successful fertilization. , It had been believed for ... Gur and Haim Breitbart (Bar-Ilan University, Israel) now show ...
... A version of a gene previously linked to ... regulate impulses, emotional memory and thinking in humans, ... National Institute of Mental Health (NIMH) have found. ... ?especially males ?tended to have relatively smaller emotion-related ...
... lactic acid is poison, a waste product that builds ... reduced performance and pain. , Some 30 years of ... a different story: Lactic acid can be your friend. ... physiologist George Brooks, UC Berkeley professor of integrative biology, ...
Cached Biology News:Aggression-related gene weakens brain's impulse control circuits 2Aggression-related gene weakens brain's impulse control circuits 3Lactic acid not athlete's poison, but an energy source - if you know how to use it 2Lactic acid not athlete's poison, but an energy source - if you know how to use it 3Lactic acid not athlete's poison, but an energy source - if you know how to use it 4
(Date:10/20/2014)... Calif. , Oct. 20, 2014 ... that the Company has signed a Notice of ... Regenerative Medicine (CIRM), effective October 1, 2014.  The ... Development payments and the release of additional grant ... CIRM grant award for clinical development of Asterias, ...
(Date:10/20/2014)... GAITHERSBURG, Md. , Oct. 20, 2014  GenVec, Inc. ... Zola P. Horovitz , Ph.D., from its board of ... GenVec board in August 2003, and served as its chairman ... also served on the Nominating and Corporate Governance and Audit ... Zola for more than a decade of dedicated service to ...
(Date:10/20/2014)... 20, 2014 Earle Martin ... announced today that Ellen Teplitzky, an experienced attorney ... the pharmaceutical industry, has joined the firm as ... legal services practice. NDA Partners provides legal ... and testimony, to top law firms and their ...
(Date:10/19/2014)... (PRWEB) October 20, 2014 ... Industry Meeting (ABIM). ABIM will take ... More information about ABIM 2014 is now ... , Delegates representing companies and organizations from ... and obtain information on the latest products ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4Zola P. Horovitz To Retire From GenVec Board 2NDA Partners Appoints Ellen Teplitzky, JD as Director of its Legal Services Practice 2
... deCODE genetics,(Nasdaq: DCGN ) today announced that it ... presentation at the JPMorgan 26th Annual Healthcare,Conference., The ... 7:30 pm GMT on,Thursday, January 10 at the Westin ... through the Investors page on deCODE,s website, http://www.decode.com, ...
... Faced with modest growth in the,traditional life ... to,opportunities in agricultural, biodefense and molecular diagnostics. ... research and,advisory firm BioInformatics, LLC today released ... preferences of scientists working in,these dynamic markets. ...
... million in ... 2007-, - sNDA submitted for ... showing favorable results - - New proteasome inhibitor, MLN2238, enters development ... 2007 net income financial guidance -, CAMBRIDGE, Mass., Jan. 4 Building ...
Cached Biology Technology:Study Examines Media Preferences of Life Scientists in Applied Markets 2Study Examines Media Preferences of Life Scientists in Applied Markets 3Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 2Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 3Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 4Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 5Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 6Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 7Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 8Millennium Announces 2008 Goals for Growth, Building on Record 2007 Results 9
... Performance The TSQ Quantum Ultra defines ... bioanalytical and environmental analysis. It features ... APCI probes, a titanium skimmer with superior ... and sensitivity. Its redesigned ion transfer ...
... Cellular uptake of long double stranded RNA ... interference in a diverse group of organisms ... RNA interference leads to the inhibition of ... of the target messenger RNA (mRNA). Attempts ...
... a tetramethylrhodamine-conjugated jetSI-ENDO (excitation at ... nm), the powerful siRNA transfection ... to determine the transfection efficiency ... and trafficking of siRNAs. More ...
... and Bruker BioSpins Metabolic Profiler is ... and analysis of complex mixtures. It ... optional Avance NMR spectrometer and integrated ... statistical analysis for applications like:,Assessment of ...
Biology Products: