Navigation Links
Family trees for yeast cells
Date:5/13/2013

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families. The team of Dr. Aime Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the renowned scientific journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads (DOI: 10.1038/nmeth.2479).

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads". To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that un
'/>"/>

Contact: Sabine Mosch
sabine.mosch@uni.lu
352-466-644-6423
University of Luxembourg
Source:Eurekalert

Page: 1 2

Related biology news :

1. New research constructs ant family tree
2. Signalling pathways meeting targeting the HER/EGFR family: Focus on breast, lung and colorectal cancers
3. FASEB SRC announces conference: TGF-β Superfamily: Signaling in Development & Disease
4. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
5. Bats split on family living
6. Networking ability a family trait in monkeys
7. All in the family: A genetic link between epilepsy and migraine
8. Bloomberg School receives $28 million for family planning advocacy
9. CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs
10. Millipede family added to Australian fauna
11. Helping family is key for social birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/19/2014)... to encourage healthier spending habits? A new study by ... by tracking the purchasing records in a supermarket chain ... nutritional value of foods for sale. , The researchers, ... and Brian Wansink PhD, author of Slim by Design ... Supermarkets in the Northeastern United States between January 2005 ...
(Date:8/19/2014)... the world, leprosy and tuberculosis live side-by-side. Worldwide ... per year, with nearly all of them occurring ... vaccine Bacille Calmette-Guerin, or BCG, provides only partial ... more potent vaccine is needed to combat both ... weapon against both diseases., In a study published ...
(Date:8/19/2014)... (Boston) Some infants born with neonatal abstinence ... a more difficult time going through withdrawal than ... understood. While genetic and epigenetic (when genes are ... identified as potential factors, researchers at Boston University ... (BMC) conducted a first of its kind study ...
Breaking Biology News(10 mins):New vaccine shows promise as stronger weapon against both tuberculosis and leprosy 2In-utero methadone, Subutex exposure could alter gene expression, cause severe Neonatal Abstience Syndrome 2
... American scientists have rediscovered a highly distinctive mammal C a ... years ago. Furthermore, it has never before been discovered in ... extinct. , The greater dwarf cloud rat (Carpomys melanurus) has ... eyes, small rounded ears, a broad and blunt snout, and ...
... throughout the sciences in the study of biological, technological, ... to visualize or understand. In a May 1 ... links in networks, Santa Fe Institute (SFI) researchers Aaron ... real-world networks can be understood as a hierarchy of ...
... Iowa and HOUSTON, Texas The first year ... at Iowa State University (ISU) has supported 26 ... to further diversify Americas sources of energy and ... third-largest integrated energy company in the United States, ...
Cached Biology News:Dwarf cloud rat rediscovered after 112 years 2Dwarf cloud rat rediscovered after 112 years 3Nature paper describes technique for extracting hierarchical structure of networks 2Iowa State-ConocoPhillips collaboration advances 26 research projects in first year 2
(Date:8/18/2014)... TORONTO , Aug. 18, 2014 /PRNewswire/ - SQI Diagnostics ... and commercializes proprietary technologies and products for advanced microarray ... call on Friday, August 22 at 2:00 pm ET.  ... , CEO, will provide a brief review of SQI,s ... Pronovost , a diagnostics industry veteran recently appointed to ...
(Date:8/18/2014)... , August 18, 2014 ... Transparency Market Research "Life Science Reagents Market(Products- PCR ... Electrophoresis, Chromatography, In-Vitro Diagnostic Reagents and Others; End ... Institutions, Clinical Laboratories, and Forensic Laboratories)- Global Industry ... - 2019", the global life science reagents market ...
(Date:8/18/2014)... , 18. August 2014 ... in eine internationale klinische Studie auf, in ... Die Linse soll das Sehvermögen bei allen ... der Operation möglicherweise keine Lese- oder Gleitsichtbrille ... dem Namen FluidVision Accommodating Intraocular Lens ...
(Date:8/15/2014)... FOREST, Ill. , Aug. 15, 2014   ... world,s leading provider of injectable drugs and infusion technologies, ... Morgan Stanley Global Healthcare Conference on Tuesday, Sept. 9, ... The presentation is scheduled to begin at 11:45 ... will be available to all interested parties through a ...
Breaking Biology Technology:SQI Diagnostics Announces Investor Conference Call with Dr. Allan Pronovost 2SQI Diagnostics Announces Investor Conference Call with Dr. Allan Pronovost 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 2Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 4Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 5Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 2Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 3Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 4
... YORK, April 17 The market for congestive ... billion in 2007, and is,expected to reach $30 ... by,Kalorama Information, Congestive Heart Failure: Major World Markets, ... three factors: an,increased incidence of CHF, the application ...
... JENA, Germany, April 17 Occlutech GmbH, the leading,European ... a recent news release., In a news release ... opinion from AIPPI the European "Association for the Protection,of ... of,certain European IP held by AGA Medical., AIPPI ...
... April 17 BioMed Realty Trust, Inc.,(NYSE: BMR ... of 5,700,000,shares of common stock at $25.50 per share. ... 22, 2008. Gross proceeds from the offering will be,approximately ... of the,offering to repay a portion of the outstanding ...
Cached Biology Technology:Genomics Likely to Replace Traditional Drug Therapies in Treating CHF 2Occlutech Clarifies Statement Regarding Favourable Opinion Obtained From AIPPI With Regard to Patents Held by AGA Medical 2BioMed Realty Trust Prices Public Offering of 5,700,000 Shares of Common Stock 2BioMed Realty Trust Prices Public Offering of 5,700,000 Shares of Common Stock 3
Whole blood is collected from healthy pigs (mixed breed, either sex) at the time of slaughter, refrigerated within 6 hours, processed within 24 hours and frozen at -20ºC....
... Rhesus Serum • Rhesus serum is collected ... 5-7 years of age. Available Anticoagulants: ... Heparin (Na) N-06: K2EDTA N-08: ... N-03: Alsevers N-05: ACD ...
Reacts with MBP from human, bovine and rat, epitope 129-138. Reacts weakly with rabbit. Does not react with guinea pig....
... DCTD_HUMAN GI Number: 4503277 ... Description: FUNCTION: Supplies the nucleotide ... dCMP + H 2 O = ... Homohexamer. SIMILARITY: Belongs to the Diseases: ...
Biology Products: