Navigation Links
Family trees for yeast cells
Date:5/13/2013

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families. The team of Dr. Aime Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the renowned scientific journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads (DOI: 10.1038/nmeth.2479).

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads". To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that un
'/>"/>

Contact: Sabine Mosch
sabine.mosch@uni.lu
352-466-644-6423
University of Luxembourg
Source:Eurekalert

Page: 1 2

Related biology news :

1. New research constructs ant family tree
2. Signalling pathways meeting targeting the HER/EGFR family: Focus on breast, lung and colorectal cancers
3. FASEB SRC announces conference: TGF-β Superfamily: Signaling in Development & Disease
4. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
5. Bats split on family living
6. Networking ability a family trait in monkeys
7. All in the family: A genetic link between epilepsy and migraine
8. Bloomberg School receives $28 million for family planning advocacy
9. CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs
10. Millipede family added to Australian fauna
11. Helping family is key for social birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/6/2014)... 6, 2014 - Insilico Medicine, Inc, a Baltimore-based ... age related diseases announced a research collaboration with ... Champions Oncology, Inc (OTC: CSBR). , "There are ... medicine, but Champions Oncology,s TumorGraft technology is unique ... chemotherapy regimen experimentally generating vast amounts of valuable ...
(Date:11/5/2014)... Kelly Redmond has dedicated his career to understanding ... a general audience. , As deputy director and ... at Nevada,s Desert Research Institute, Redmond has more ... application and dissemination of climate data to the ... Meeting in San Francisco on December 15-19, 2014 ...
(Date:11/4/2014)... LAKE CITY, Nov. 5, 2014 – University of Utah ... enzymes to help jet fuel produce electricity without needing ... be used to power portable electronics, off-grid power and ... online today in the American Chemical Society journal ... electricity through a chemical reaction between a fuel and ...
Breaking Biology News(10 mins):Insilico Medicine, Inc announces research collaboration with Champions Oncology, Inc 2Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2Jet-fueled electricity at room temperature 2
... for the first time, how a potted plant,s roots are arranged ... be presented at the Society for Experimental Biology meeting on 30th ... makes plants grow over 40% larger. From their 3-D ... their roots to the pot,s walls. It is likely that the ...
... new batch of Geology papers posted online ... asynchrony; oceanic crust; sand budgets; the Alpine fault,s seismic ... weathering in the Critical Zone; giant wave ripples; the ... network; and soils as ledgers recording transactions of energy ...
... apart DNA that bears certain signature sequences is a defense ... can be a powerful research tool. With a newly discovered ... a targeted method of slicing DNA that they say can ... lab. Tools that snip apart DNA strands in defined ...
Cached Biology News:Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 2Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 3Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 4Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 5Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 6Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 7Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 8Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 9Sandy beaches, hydrocarbon reservoirs, tectonic tilting: It's all about geology 10Programmable RNA complex could speed genome editing in the lab 2Programmable RNA complex could speed genome editing in the lab 3
(Date:11/22/2014)... , November 21, 2014 ... wechselt als Chief Commercial Officer zu KLOX    ... der Unternehmensentwicklung und Chief Financial Officer bestellt    ... mit der Vermarktung seines jüngst zugelassenen Behandlungssystems zur Wundheilung ... ("KLOX" bzw. das "Unternehmen") ist sehr erfreut, die folgenden ...
(Date:11/21/2014)... 20, 2014 Offering a complete line ... Entris Balance promotion . The Sartorius Entris Balance is ... high accuracy, and easy-to-maintain balance entry level balance. , ... was designed to help customer’s bridge the gap between ... that is very durable. Sartorius is the second biggest ...
(Date:11/21/2014)... NY (PRWEB) November 20, 2014 The ... now available as an open access journal featuring the ... infrared fluorescent proteins to preserve photoresponsiveness in the retina ... Two-color fluorescent in situ hybridization using chromogenic substrates in ... for DNA, RNA, and protein electrophoresis , ...
(Date:11/21/2014)... 2014 2014 Deep Research Report ... in-depth research report on the transformer market. The ... application, and industry chain structure as well as ... analysis, including China’s domestic market as well as ... situation analysis. The report also covers information on ...
Breaking Biology Technology:KLOX Technologies meldet Neubesetzung von Führungspositionen 2KLOX Technologies meldet Neubesetzung von Führungspositionen 3KLOX Technologies meldet Neubesetzung von Führungspositionen 4KLOX Technologies meldet Neubesetzung von Führungspositionen 5Pipette.com Announces an Extension on the Sartorius Entris Balance Promotion to the End of 2014 2BioTechniques: The International Journal of Life Science Methods Announces the Publication of the November 2014 Issue—Available in Print and Online 2BioTechniques: The International Journal of Life Science Methods Announces the Publication of the November 2014 Issue—Available in Print and Online 3International Transformer Industry - Analysis on Applications, Development & Forecasts Now Available at ReportsnReports.com 2International Transformer Industry - Analysis on Applications, Development & Forecasts Now Available at ReportsnReports.com 3
... /PRNewswire-FirstCall/ - MIGENIX Inc.,(TSX: MGI; OTC: MGIFF), ... reports financial results for the three months ... its programs., UPDATE ON DRUG DEVELOPMENT ... cationic peptide;,prevention of catheter-related infections): A pivotal ...
... a Planned Phase II Study of ... Sclerosis, COPENHAGEN, Denmark, December 13 Genmab A/S,(OMX: ... study of,ofatumumab (HuMax-CD20(R)) for the treatment of relapsing remitting,multiple ... study which will be conducted under Genmab,s collaboration with,GlaxoSmithKline ...
... SAN FRANCISCO, Calif., Dec. 13 Anesiva,Inc. (Nasdaq: ... a common stock offering,of 11,111,111 shares of its ... discount to the closing bid price on December ... before deducting underwriting,discounts and commissions (approximately $50 million ...
Cached Biology Technology:MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 2MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 3MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 4MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 5MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 6MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 7MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 8MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 9MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 10MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 11MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 12MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 13MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 14MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 15MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 16MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 17MIGENIX Reports Second Quarter Fiscal Year 2008 Financial Results 18Genmab Announces Details of Planned Ofatumumab Phase II Study in Multiple Sclerosis 2Genmab Announces Details of Planned Ofatumumab Phase II Study in Multiple Sclerosis 3Anesiva Raises $45 Million in Common Stock Offering 2
... α chain (human), clone 41D ... Immunogen : Native clusterin purified from ... 7.4, 0.15M NaCl and 0.05% sodium ... RIPA lysates from HeLa cells or ...
Rabbit polyclonal to Methionine Aminopeptidase 2 ( Abpromise for all tested applications). entrezGeneID: 10988 SwissProtID: P50579...
Chlamydia pneumoniae (TWAR)...
Mouse anti-Insulin-Like-3 Class: Antibody Product Group: Adipokine and Obesity related...
Biology Products: