Navigation Links
Family trees for yeast cells
Date:5/13/2013

Researchers at the Institute for Systems Biology in Seattle (USA) and the Luxembourg Centre for Systems Biomedicine (LCSB) at the University of Luxembourg have jointly developed a revolutionary method to analyse the genomes of yeast families. The team of Dr. Aime Dudley from the ISB and Dr. Patrick May from LCSB published their paper in the renowned scientific journal Nature Methods on May 12th. It describes a new method called BEST: Barcode Enabled Sequencing of Tetrads (DOI: 10.1038/nmeth.2479).

Baker's yeast is one of the most powerful and widely used organisms in genetic research because of the ability to perform a technique called tetrad analysis. Matings between two yeast "parents" produce four yeast "children" or spores that stick together as "tetrads". To genetically analyse these progeny, one needs to physically separate ("dissect") the four spores of each tetrad. The current method for doing this is extremely labour-intensive and has remained essentially unchanged for 75 years. A number of methods have tried to circumvent this manual process, but until now, none has been able to capture a crucial piece of information, the tetrad relationships between the spores.

The research team designed a new method that is several hundred times faster and is based on the following principles: First, two DNA sequences are added to the cells: A short sequence, called a barcode, that labels spores that come from the same tetrad and additionally a GFP (Green Fluorescent Protein) reporter gene. This fluorescent reporter allows the separation of tetrads away from other types of yeast cells by FACS sorting. Then, the four spores of the tetrad are disrupted using a specific enzymatic reaction and allowed to grow up as individuals. Ultimately, the barcode and the same 2-3% of each individual's genome are sequenced allowing the spore strain to be placed in the correct position of the family tree for further genetic analysis.

"Identifying the genes that un
'/>"/>

Contact: Sabine Mosch
sabine.mosch@uni.lu
352-466-644-6423
University of Luxembourg
Source:Eurekalert

Page: 1 2

Related biology news :

1. New research constructs ant family tree
2. Signalling pathways meeting targeting the HER/EGFR family: Focus on breast, lung and colorectal cancers
3. FASEB SRC announces conference: TGF-β Superfamily: Signaling in Development & Disease
4. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
5. Bats split on family living
6. Networking ability a family trait in monkeys
7. All in the family: A genetic link between epilepsy and migraine
8. Bloomberg School receives $28 million for family planning advocacy
9. CEVA, Rubidium Partner to Deliver Speech Processing Solutions based on the CEVA-TeakLite Family of DSPs
10. Millipede family added to Australian fauna
11. Helping family is key for social birds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2015)... NexID Biometrics LLC, whose spoof-mitigation technology boosts protection ... beginning of shipments of version 2.0 of its industry ... in Potsdam, N.Y. , is making ... here today at the Walter E. Washington Convention Center. ... boosts the accuracy rate range to 96.5 to 99.5 ...
(Date:3/23/2015)... 23, 2015   HOYOS Labs , a leading ... announced that the Company will demonstrate multiple biometrics-based technologies ... consumers at Connect:ID on March 23 through 25, 2015, ... will highlight the IEEE Biometric Open Protocol Standard; free ... enterprise access control system. BOPS was invented by HOYOS ...
(Date:3/20/2015)... 2015 Research and Markets ( ... "India Sensors Market Forecast and Opportunities 2020" ... sensor market is projected to grow at a ... electronics, automotive, industrial and healthcare sectors are the ... In addition, adoption of MEMS technology in sensors ...
Breaking Biology News(10 mins):NexID Begins Shipments Of Version 2.0 Fake-Finger-Detection Solution 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 3
... and Shenzhen, China, September 30, 2013 - China,s genomics ... the initiation of a joint project to unravel the ... of over $900k, the project will be one of ... involve experts from the IPATIMUP, a leading cancer research ...
... Pace recognizes that we are at a critical time in ... decrease in new HIV infections worldwide and increasing numbers of ... and vaccine research and new evidence on Treatment as Prevention, ... Sharon Lewin, Local Co-Chair of AIDS 2014, Head of the ...
... the human body, particularly neuroproteins that maintain proper brain ... and Parkinson,s are known as "tangle diseases" because they ... in the brain. A team of Australian and ... BMAA can be inserted into neuroproteins, causing them to ...
Cached Biology News:New mechanism for protein misfolding may link to ALS 2
(Date:3/27/2015)... LANSING, Mich. , March 27, 2015  Neogen ... Properties, LLC, has adopted a prearranged trading plan in ... James Herbert , Chairman and CEO of Neogen Corporation, ... shares of Neogen Corporation common stock. Herbert ... the disclosure was appropriate. The filing is only for ...
(Date:3/27/2015)... , March 27, 2015  CASI Pharmaceuticals, Inc. ... the acquisition, development and commercialization of innovative therapeutics addressing ... market with a commercial focus on China ... and 12 months ended December 31, 2014.  ... million), or ($0.05) per share, for the three months ...
(Date:3/26/2015)... The Market Element LLC, a leading inbound ... Designing Gig LLC to provide website design, development, and ... this alliance with Designing Gig represents a pivotal moment ... comprehensive, end-to-end, digital solution for our clients, which now ... Miller, president of The Market Element. “For small and ...
(Date:3/26/2015)... 26, 2015 ReliantHeart, Inc., an innovative ... a supplier of precision transit-time flow measurement solutions, are ... improve the efficiency of the HeartAssist5® Left ... to experience greater mobility and peace of mind. ... flow board, will draw 1/6th of the power required ...
Breaking Biology Technology:Neogen CEO adopts 10b5-1 Trading Plan 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 2CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 3CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 4CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 5CASI Pharmaceuticals Reports 2014 Fourth Quarter And Full Year Financial Results 6The Market Element LLC and Designing Gig LLC Form Strategic Alliance 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 2LVAD patients to have greater mobility and peace of mind through Transonic and ReliantHeart’s True Flow collaboration for the HeartAssist5® 3
... 4 /Xinhua-PRNewswire-FirstCall/ -- Genesis,Pharmaceuticals Enterprises, Inc. (OTC Bulletin ... company in the People,s Republic,of China, today announced ... its common stock which will begin trading under ... Genesis, common stock will no longer trade,under the ...
... Shandong, China, Sept. 4 /Xinhua-PRNewswire-FirstCall/ --,China Biologic ... Biologic",or the "Company"), one of the leading ... China (the "PRC"), today announced that on,August ... Drug Administration (the "SFDA"),granted China Biologic,s production ...
... EDMONTON, Sept. 4 /PRNewswire-FirstCall/ - BioMS Medical Corp. ... multiple sclerosis (MS), today,announced that the Food and ... fast track designation for the Company,s lead drug, ... (SPMS). Dirucotide,(MBP8298) is currently being evaluated in a ...
Cached Biology Technology:Genesis Pharmaceuticals Completes Reverse Stock Split and Will Trade Under a New Symbol 2Genesis Pharmaceuticals Completes Reverse Stock Split and Will Trade Under a New Symbol 3China Biologic's Production Facility Renews GMP Certification 2China Biologic's Production Facility Renews GMP Certification 3BioMS Medical's lead drug, dirucotide (MBP8298) for the treatment of multiple sclerosis, receives fast track designation from FDA 2BioMS Medical's lead drug, dirucotide (MBP8298) for the treatment of multiple sclerosis, receives fast track designation from FDA 3BioMS Medical's lead drug, dirucotide (MBP8298) for the treatment of multiple sclerosis, receives fast track designation from FDA 4
Ps-Aeruginosa (IgA) -Ab EIA Sample Size: 100 l...
Request Info...
Candida (IgG) -Ab EIA Sample Size: 5 l...
... (Competitive) EIA Principle: The Oxidized LDL ... 4E6. Oxidized LDL in the sample competes with ... the microtiter well for the binding of the ... Sample Size: 25 l The Oxidized ...
Biology Products: