Navigation Links
Embryonic stem cell strategy advanced with UCSF finding
Date:9/11/2007

lls in the culture dish. His team showed that over-expressing these genes oct4, sox2, klf4 and c-myc can cause the full complement of genes in mouse cells to lose their adult functions and begin functioning as they would have as embryonic stem cells. Yamanaka named these cells induced pluripotent (iPS) cells.

But because only a very low percentage of cells complete reversion to the embryonic stem cell state with this technique, and because the cells are situated among millions of cells in the culture dish that do not complete the transformation, the scientists had a difficult time identifying the fully reprogrammed cells. Thus, they developed the technique of inserting the foreign drug-resistance gene into the mouse skin cells. This gene was designed to only switch on in cells that completed the reversion to the embryonic stem cell state. With addition of the drug to the culture dish, the vast majority of cells, those that had not reverted to embryonic stem cells, died. Only those that had reverted survived and could then be expanded.

With the alternative technique developed by the UCSF team, the efficiency of embryonic stem cell production remained low. However, the mouse skin cells that did start to revert to embryonic stem cells could readily be identified by their form and structure in the absence of any drug. The researchers went on to show that these cells indeed behaved like embryonic stem cells and could give rise to all cell types of the body.

Separately, the team demonstrated that reprogramming could be achieved when one of the four genes over-expressed to initiate reprogramming -- c-myc -- was replaced with a related gene, known as n-myc. These genes are involved in the formation of different tumors, so by beginning to replace genes in this method the researchers may find combinations of reprogramming genes that are safer, says Blelloch.

Studies should address the relative efficacy of n-myc versus c-myc in reprogram
'/>"/>

Contact: Jennifer O'Brien
jobrien@pubaff.ucsf.edu
415-476-2557
University of California - San Francisco
Source:Eurekalert

Page: 1 2 3 4

Related biology news :

1. Priming embryonic stem cells to fulfill their promise
2. Current human embryonic stem cell lines contaminated UCSD/Salk team finds
3. Transplantation Of Monkey Embryonic Stem Cells Reverses Parkinson Disease In Primates
4. UCSD Discovery Shows How Embryonic Stem Cells Perform Quality Control Inspections
5. Researchers devise way to mass-produce embryonic stem cells
6. Protein helps regulate the genes of embryonic stem cells
7. Embryonic Stem Cells Treated With Growth Factor Reverse Hemophilia In Mice: UNC Researchers
8. Vitamin As paradoxical role in influencing symmetry during embryonic development revealed
9. Human embryonic stem cells have the potential to develop into eggs and sperm in the laboratory
10. Canadas first embryonic stem cell lines
11. Researchers make embryonic-like stem cells from umbilical cord blood
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/2/2014)... accidentally touch a hot oven, you rapidly pull your ... involved in sensing and responding to such painful stimuli, ... Duke researchers have made a surprising discovery about the ... worms, and have built a structural model of the ... Communications , may help direct new strategies to treat ...
(Date:9/1/2014)... ─ Dietary quality in the U.S. has improved steadily ... fat intakebut overall dietary quality remains poor and disparities ... to a new study from Harvard School of Public ... evidence to date that the extensive efforts by many ... having some payoff, but it also indicates that these ...
(Date:9/1/2014)... Within minutes after birth, every child in the U.S. ... host of conditions, including sickle cell disease. Thousands of ... lucky, meaning many suffer and die from the disease ... a halt to at least some of those deaths. ... Biology working in the lab of George Whitesides, the ...
Breaking Biology News(10 mins):Surprising new role for calcium in sensing pain 2Surprising new role for calcium in sensing pain 3Quality of US diet shows modest improvement, but overall remains poor 2Quality of US diet shows modest improvement, but overall remains poor 3Faster, cheaper tests for sickle cell 2Faster, cheaper tests for sickle cell 3
... connection between the body, mind and spirit has been ... years. The notion that psychosocial and societal considerations have ... crystallized as a result of the experiences of a ... writer and magazine editor of the popular Saturday Review, ...
... / b3c newswire / - Addressing significant product safety and ... has announced the commercial launch of two protease-free ... Albumin-DX (Lipid-Free) perform better in diagnostics applications than ... serum albumin (pHSA).  , , ...
... is a tragic disease that robs an individual of their memory ... 65 now suffer from the disease and one in two people ... Alzheimer,s does not only affect the elderly. Familial Alzheimer,s disease (FAD), ... The Study , Alzheimer,s is a complex ...
Cached Biology News:Laughter remains good medicine 2Laughter remains good medicine 3InVitria Announces Launch of Recombinant Albumin for Diagnostics 2Computational model examines the pathways of Alzheimer's that strikes at the young 2
(Date:8/29/2014)... 29, 2014  Pfenex Inc. (NYSE MKT: PFNX), ... of high-value and difficult to manufacture proteins including ... reported financial results for the second quarter ended ... completion of our initial public offering, we have ... product candidates, derived from our proprietary protein expression ...
(Date:8/29/2014)... 29, 2014 The global companion ... in 2019. It is expected to grow at ... and was valued at $1.8 billion in 2013, ... Market Research. , For more information regarding analysis ... , The research report, titled “Companion Diagnostics Market ...
(Date:8/29/2014)... Pittsburgh, PA (PRWEB) August 29, 2014 ... Chairman, Hub MacDonald, and colleague Koichiro Matsuda, Horiba Scientific, ... JASIS on September 5, 2014. JASIS , Asia’s ... September 3-5, 2014, in Makuhari Messe, Japan. , ... New Tools for Bioanalysis from Single Molecules to Single ...
(Date:8/29/2014)... 2014 Local veterinarian, Dr. Keith Clement, ... seeking candidates to participate in an investigational study of ... ultimate goal of this study is to determine if ... or two arthritically affected joints can help reduce pain ... for the current investigational study must be older than ...
Breaking Biology Technology:Pfenex Reports Second Quarter 2014 Results and Provides Business Update 2Pfenex Reports Second Quarter 2014 Results and Provides Business Update 3Pfenex Reports Second Quarter 2014 Results and Provides Business Update 4Pfenex Reports Second Quarter 2014 Results and Provides Business Update 5Pfenex Reports Second Quarter 2014 Results and Provides Business Update 6Pfenex Reports Second Quarter 2014 Results and Provides Business Update 7Pfenex Reports Second Quarter 2014 Results and Provides Business Update 8Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 2Companion Diagnostics Market to Hit $5.6 Billion in 2019: Transparency Market Research 3Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2Burnt Hills Veterinary Hospital Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... today its financial results for the year ending 31,December 2008. , , ... regulatory process for intravenous iclaprim in US, - ... of "intravenous-to-oral" Phase II trial with oral, ... - Share placing, Cash and financial investments of ...
... better restaurant menu optionsINDIANAPOLIS, Feb. 25 This year,s ... America with the reduction of nearly a half billion ... three years. Since the market introduction in 2006, Omega-9 ... replaced more than 340 million pounds of trans fat, ...
... collaborative agreement with CureDM to continue the preclinical development of CureDM,s HIP2B ... , ... Cary, NC (PRWEB) February 25, 2009 -- Calvert ... the signing of a collaborative agreement with CureDM to continue ...
Cached Biology Technology:Arpida Announces Full Year 2008 Financial Results 2Arpida Announces Full Year 2008 Financial Results 3Arpida Announces Full Year 2008 Financial Results 4Arpida Announces Full Year 2008 Financial Results 5Heart Health Month Milestone: Half a Billion Pounds of Bad Fats Eliminated From North American Diet 2Heart Health Month Milestone: Half a Billion Pounds of Bad Fats Eliminated From North American Diet 3Calvert Research and CureDM Sign Collaborative Agreement 2Calvert Research and CureDM Sign Collaborative Agreement 3
Mouse monoclonal antibody raised against a partial recombinant DENR. NCBI Entrez Gene ID = DENR...
Mouse monoclonal antibody raised against a partial recombinant CRKRS. NCBI Entrez Gene ID = CRKRS...
... BD PhosFlow Perm/Wash Buffer I can be ... signaling proteins to permeabilize cells and to ... wash buffer. Because saponin-mediated cell permeabilization is ... keep the cells in the presence of ...
Mouse monoclonal antibody raised against a full length recombinant GPT. NCBI Entrez Gene ID = GPT...
Biology Products: