Navigation Links
Ebola virus disarmed by excising a single gene
Date:1/21/2008

la is a genetic pauper. It has only eight genes and depends on host cells to provide much of the molecular machinery to make it a successful pathogen. The virus's VP30 gene makes a protein that enables it to replicate in host cells. Without the protein, the virus cannot grow.

"The altered virus does not grow in any normal cells," says Kawaoka. "We made cells that express the VP30 protein and the virus can grow in those cells because the missing protein is provided by the cell."

It took years, Kawaoka explains, to find which viral protein was not toxic to cells and could thus be used to develop a system, using monkey kidney cells, to confine the virus.

And Kawaoka, an internationally noted virologist, is convinced of the safety of the new system: "We did this work in a BSL 4, and the altered cells didn't produce any infectious virus after many passages or replication cycles."

With the exception that it is unable to grow in anything but cells engineered to express the VP30 protein, the virus is identical to the pathogen found in the wild, making it ideal for studies of basic biology, vaccine development and screening for antiviral compounds.

"This system can be used for drug screening and for vaccine production," Kawaoka says, noting that getting the equipment and compounds for such work into a BSL 4 lab is extremely difficult. "High throughput screening (for drugs) in a BSL 4 is almost impossible."

Currently, live Ebola virus can be studied only in a BSL 4 laboratory. Any proposal to permit studying the pathogen in lower safety level labs is certain to generate controversy.

But according to Kawaoka, making the agent available for study to a broader cross section of science is essential for thwarting the virus that kills a high percentage of its victims because there is now no defense against it. A new strain of Ebola, which so far has emerged only in remote areas of the world, was recently identified in U
'/>"/>

Contact: Yoshihiro Kawaoka
kawaokay@svm.vetmed.wisc.edu
608-265-4925
University of Wisconsin-Madison
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Features of replication suggest viruses have common themes, vulnerabilities
2. First all-African GM crop is resistant to maize streak virus
3. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
4. Gamma globulin effective in treating eye infections caused by adenoviruses
5. Discovery may help defang viruses
6. Who will recover spontaneously from hepatitis C virus infection
7. Prions and retroviruses -- an unholy alliance?
8. Scientists learn structure of enzyme in unusual virus
9. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
10. New field-deployable biosensor detects avian influenza virus in minutes instead of days
11. Depression, aging, and proteins made by a virus may all play role in heart disease
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/16/2014)... TALLAHASSEE, Fla. Florida State University researchers have identified ... deterioration of bone density and muscle mass with obesity. ... heavier you were the better your bones would be ... Ilich-Ernst, the Hazel Stiebeling Professor of Nutrition at Florida ...
(Date:4/16/2014)... Sometimes known as Kennedy,s disease, spinal and bulbar muscular atrophy ... progressive muscle weakness and atrophy. Researchers have long considered it ... the cells in the spinal cord and brainstem that control ... the April 16, 2014 online issue of Neuron , ...
(Date:4/16/2014)... , , Everything we do all of our movements, thoughts ... one another, and recent studies have suggested that some of ... known as astrocytes may be listening in on, or even ... study suggests that astrocytes might only be tuning in part ...
Breaking Biology News(10 mins):Researchers: Obesity can amplify bone and muscle loss 2Mutant protein in muscle linked to neuromuscular disorder 2Eavesdropping on brain cell chatter 2Eavesdropping on brain cell chatter 3
... BIO-key International, Inc.,(OTC Bulletin Board: BKYI), a leader ... that the U.S. Patent and Trademark,Office has approved the ... within a Biometric Security System" method. , ... protected by this patent (patent number,7,454,624) provide another layer ...
... Ore. December 3, 2008. The first 5-year forest inventory ... available to the public: California,s Forest Resources, 2001-2005: Five-Year ... findings from the report:, , Forests cover about ... acres is publicly managed. , Houses built in the ...
... Institution of Oceanography at UC San Diego returning ... details of vibrant sea life and ecosystems in ... species and marine animals previously never seen alive. ... depths, have revealed disturbing declines in sea-life populations ...
Cached Biology News:BIO-key(R) Granted Patent to Secure Biometric Enrollments From Unauthorized Use 2Forest inventories in California include more than trees 2Forest inventories in California include more than trees 3Expeditions reveal gulf of California's deep sea secrets, as well as human imprints 2Expeditions reveal gulf of California's deep sea secrets, as well as human imprints 3Expeditions reveal gulf of California's deep sea secrets, as well as human imprints 4
(Date:1/15/2014)... 2014 Freeslate, Inc ., the ... that Lupin Limited, one of India’s top five pharmaceutical ... PharmD System for high throughput solid form screening. ... on a wide range of quality, affordable generic and ...
(Date:1/14/2014)... 2014 Histogen, Inc., a regenerative medicine ... grown under simulated embryonic conditions, today announced that they ... Medical, Inc. for physician-dispensed aesthetic products containing Histogen’s proprietary ... agreement is an amendment to the existing license between ...
(Date:1/14/2014)... BETHESDA, Md. , Jan. 14, 2014  Organovo announced ... from the National Institutes of Health (NIH) to help ... effective treatments to patients on a faster timeline. Organovo ... Sciences (NCATS) and the National Eye Institute (NEI) to ...
(Date:1/14/2014)... 14, 2014   Kinaxis ®, provider of RapidResponse ®, ... and operations planning ( S&OP ) service, is proud to ... which will be held at the Hilton San Diego Bayfront, ... customer Elisabeth Kaszas , Director of Supply Chain at ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3Organovo Announces Collaboration with National Institutes of Health 2Organovo Announces Collaboration with National Institutes of Health 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... VIGILAIR Systems, an industry leader in Ultraviolet ... published, and first of its kind hospital study that ... (HAI) with the application of VIGILAIR® air cleaning technology ... The published article , Effect of enhanced ultraviolet germicidal ...
... BioNeutral Group, Inc. (OTCBB: BONU), a specialty life science ... J.D., as a new member to its Board of Directors. ... Wharton School of Business, University of Pennsylvania and received his ... been an attorney in the state of New Jersey since ...
... Sangamo BioSciences, Inc. (Nasdaq: SGMO ) announced ... its common stock pursuant to an effective shelf registration statement ... sole book-running manager in this offering and Sangamo will grant ... to 1,005,000 additional shares. The securities described ...
Cached Biology Technology:Newly Published Study Shows Dramatic Reduction in Hospital Acquired Infections 2BioNeutral Appoints Michael D. Francis, J.D. as Director to the Board 2BioNeutral Appoints Michael D. Francis, J.D. as Director to the Board 3Sangamo Announces Public Offering of Common Stock 2Sangamo Announces Public Offering of Common Stock 3
Mouse monoclonal antibody raised against a partial recombinant DHX8. NCBI Entrez Gene ID = DHX8...
... Mouse polyclonal antibody raised ... Immunogen: ... 241 a.a) partial recombinant protein ... Accession Number: NM_004693 ...
... DNA Visualizer DX's sensitivity of DNA detection has ... band in an agarose gel can be detected ... kit can be purified by organic solvent extraction ... commercially kits worked well.) DNA excised from ...
Mouse monoclonal antibody raised against a full length recombinant CSNK2A2. NCBI Entrez Gene ID = CSNK2A2...
Biology Products: