Navigation Links
Development of a FRET sensor for real-time imaging of intracellular redox dynamics
Date:6/7/2011

In work published in the June 2011 issue of Experimental Biology and Medicine, Kolossov, Spring and their co-investigators - a multidisciplinary team within the Institute for Genomic Biology at the University of Illinois - have transferred the concept of redox-sensitive Green Fluorescent Proteins (GFPs) to a quantitative Frster resonance energy transfer (FRET) imaging platform. For the FRET-based sensors, a change in redox induces a conformational change in a redox-sensitive switch that links two fluorescent proteins (the donor and acceptor), changing their distance, which in turn causes a detectable change in FRET efficiency. In its oxidized state the wavelength spectrum of the sensor's fluorescence emission is red-shifted (due to increased acceptor fluorescence), independent of variations in the local sensor concentration or in the intensity of the excitation light. As explained by Robert Clegg, a pioneer in the development of novel applications of optical microscopy in the biological sciences and key collaborator on the study, "FRET-based sensors circumvent the complications associated with imaging methods based on fluorescence intensity, since the increase in the FRET acceptor molecule's fluorescence can only take place if there is a change in the efficiency of energy transfer. This specific and discriminatory feature of FRET is one of the driving motives behind our development of a FRET-based assay rather than relying only on changes in the fluorescent intensity of a single component."

The current publication builds on the authors' previous work, where they reported a series of first-generation redox-sensitive linkers flanked by FRET donor and acceptor GFP-variants. As summarized by co-author Vladimir Kolossov, "The major advance in the current study is an improved dynamic range of the spectroscopic signal; in other words, a greater difference between fully reduced and oxidized states. Increasing the dynamic range leads to better discrimin
'/>"/>

Contact: Vladimir L. Kolossov
viadimer@illinois.edu
Society for Experimental Biology and Medicine
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Developmental disease is recreated in an adult model
2. Ulcer bacteria may contribute to development of Parkinsons disease
3. A study will enable the survival and growth of the 3 development phases of the European eel
4. Building brains: An introduction to neural development
5. Gene variant linked with development of COPD in men
6. Scientists find new class of compounds with great potential for research and drug development
7. New organic catalyst should enhance drug research and development
8. Brain development goes off track as vulnerable individuals develop schizophrenia
9. Study: Pace of brain development still strong in late teens
10. Spikemoss genome offers new paths for biofuels research -- bridges plant development gap
11. Brain cell migration during normal development may offer insight on how cancer cells spread
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/7/2014)... BURBANK, Calif. , Nov. 7, 2014  In conjunction ... Burbank Police Departments, The Community Foundation of the ... BURBANK DNA JUSTICE PROJECT," a $250,000 initiative to ... successful initial phase. In its first six months of existence, ... desperately needed evidence support for local law enforcement. ...
(Date:11/6/2014)... asset for a predator. Except when that predator runs so ... relative to its size, is the fastest creature on Earth. ... per second (at about five miles per hour). The fastest ... sprinting gold from the tiger beetle, a person would have ... beetle has a problem. At peak speeds, everything becomes a ...
(Date:11/5/2014)... show great diversity in their ability to identify scents ... differ in their perceptual evaluation of odors, with women ... , Sex differences in olfactory detection may play ... connected to one,s perception of smell, which is naturally ... superiority has been suggested to be cognitive or emotional, ...
Breaking Biology News(10 mins):Community Foundation Of The Verdugos Unveils Campaign To Fund Glendale-Burbank DNA Justice Project 2The tiger beetle: Too fast to see 2The female nose always knows: Do women have more olfactory neurons? 2
... Amsterdam, November 17, 2009 Brain imaging can offer a ... A study conducted at the University of Kansas School of ... of even those who are healthy, but carry some risk ... the November 2009 issue of the Journal of Alzheimer,s ...
... Two University of New Hampshire assistant professors ... Early Career Development (CAREER) grants. Vaughn Cooper of ... received $1 million to better understand beneficial mutations ... data collection. Christopher White of the mechanical engineering ...
... available in German . , Scientists from ... Munich, Germany, have now deciphered the mechanism that illustrates how ... car, these cells have an engine, a clutch and wheels ... group leader at the MPI of Biochemistry. The results, which ...
Cached Biology News:2 UNH faculty receive $1.4M in CAREER grants from NSF 2Cross-country runabouts -- immune cells on the move 2
(Date:11/26/2014)... 25, 2014 Whitehouse Laboratories is ... the Morris County (New Jersey) Chamber of Commerce ... leading business organization in the North Jersey area, ... engage some key clients in the pharmaceutical and ... geographic area. Membership enables Whitehouse Laboratories a higher ...
(Date:11/26/2014)... Continuing its award-winning streak, Nerium International, the ... Awards for outstanding creative achievement by its marketing and ... the organization’s top honor, in the Branding category for ... April of this year. The event showcased top speakers ... was awarded a Gold MarCom Award in the Special ...
(Date:11/26/2014)... (PRWEB) November 25, 2014 Miles Holder, ... joined the Graphel Carbon Products team as Sales/Marketing Manager. ... marketing, and customer service for the graphite industry. , ... to our team,” stated Dave Trinkley, VP of Market ... wide range of experience in the graphite industry, along ...
(Date:11/26/2014)... November 26, 2014 2014 Deep ... an in-depth research report on the Global Palmitic ... introduces Palmitic Acid, basic information, including definition, classification, ... and news analysis, etc. , For international ... Palmitic Acid market in China and other ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 3
... optimizing electroporation., , , , , , , , ... Pulse length , , ... , of the electroporation buffer , Influence , ... of temperature , Influence , ...
... , , , , ... in most laboratories that RNA is more susceptible , ... metal ions for activity (and can therefore easily be inactivated ... (RNases) have virtually no cofactor , requirements and ...
... TM Help ? , , , , , , ... , , As ... that is performed everyday, for a good portion , of ... Due to the nature of the tests being conducted, only short breaks , ...
Cached Biology Technology:Optimizing electroporation parameters 2Optimizing electroporation parameters 3Optimizing electroporation parameters 4Optimizing electroporation parameters 5Decontamination and inhibition of ribonucleases 2Decontamination and inhibition of ribonucleases 3Decontamination and inhibition of ribonucleases 4Ergonomics and Liquid Handling, How can BrinkmannTM Help ? 2Ergonomics and Liquid Handling, How can BrinkmannTM Help ? 3Ergonomics and Liquid Handling, How can BrinkmannTM Help ? 4
... versatile ST-195 allows real-time observations of cell ... instrument has 64 different strain parameters in ... The thin silicon strain chamber used by ... and fluorescence microscopy. ST-195 can be used ...
Immunogen GST fusion protein corresponding to full-length rat JNK3/ SAPK1b. Quality Assurance Routinely evaluated by immunoblot. Stability 2 years at -20°C from date of shipment GenBank A...
... The EasyCount ViaSure Kit is used in ... and live nucleated cells automatically. The ViaSure ... acid dyes - Acridine Orange and Ethidium Bromide ... a single test, while the EasyCount Slide 6 ...
... a powerful yet simple technology for labeling ... kit provides the heterobifunctional linkers SFB and ... efficiencies. It enables conjugation of various tags ... with a variety of proteins. , ...
Biology Products: