Navigation Links
Developing microbial cell factories by employing synthetic small regulatory RNAs
Date:1/20/2013

Biotechnologists have been working hard to address the climate change and limited fossil resource issues through the development of sustainable processes for the production of chemicals, fuels and materials from renewable non-food biomass. One promising sustainable technology is the use of microbial cell factories for the efficient production of desired chemicals and materials. When microorganisms are isolated from nature, the performance in producing our desired product is rather poor. Metabolic engineering is performed to improve the metabolic and cellular characteristics to achieve enhanced production of desired product at high yield and productivity. Since the performance of microbial cell factory is very important in lowering the overall production cost of the bioprocess, many different strategies and tools have been developed for the metabolic engineering of microorganisms.

One of the big challenges in metabolic engineering is to find the best platform organism and to find those genes to be engineered so as to maximize the production efficiency of the desired chemical. Even Escherichia coli, the most widely utilized simple microorganism, has thousands of genes, the expression of which is highly regulated and interconnected to finely control cellular and metabolic activities. Thus, the complexity of cellular genetic interactions is beyond our intuition and thus it is very difficult to find effective target genes to engineer. Together with gene amplification strategy, gene knockout strategy has been an essential tool in metabolic engineering to redirect the pathway fluxes toward our desired product formation. However, experiment to engineer many genes can be rather difficult due to the time and effort required; for example, gene deletion experiment can take a few weeks depending on the microorganisms. Furthermore, as certain genes are essential or play important roles for the survival of a microorganism, gene knockout experiments cannot be p
'/>"/>

Contact: Lan Yoon
hlyoon@kaist.ac.kr
82-423-502-295
The Korea Advanced Institute of Science and Technology (KAIST)
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Developing second skin military fabric to repel chemical and biological agents
2. Inspired: Canada funds 68 bold, inventive ways to improve health, save lives in developing countries
3. Watching the developing brain, scientists glean clues on neurological disorder
4. Cilia guide neuronal migration in developing brain
5. First-ever 3-D stress map of developing embryonic heart sheds light on why defects form
6. The role of stem cells in developing new drugs
7. Sam Houston state developing lab test for bath salts
8. Purple corn compound may aid in developing future treatments for Type 2 diabetes, kidney disease
9. Risk of developing diabetes higher in neighborhoods that arent walk-friendly: Study
10. Students create low-cost biosensor to detect contaminated water in developing nations
11. UC Riverside developing biofuel formulations for California
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/14/2014)... published in Cancer Research shows SIRT6—a protein ... cancers—can promote the development of skin cancers by turning ... of sun-damaged skin cells. , Previously considered protective, SIRT6 ... sirtuins that help regulate genomic stability and prevent some ... repair DNA damage, which can lead to cancer. This ...
(Date:10/14/2014)... the house fly genome for the first time, revealing ... insect that thrives in pathogen-rich dung piles and garbage ... journal Genome Biology , will increase understanding of ... quickly adapt to resist insecticides, which could lead to ... carry and transmit more than 100 human and animal ...
(Date:10/14/2014)... Mount Sinai-led research team has discovered a new kind of ... a cell that lines liver blood vessels, according to a ... . The existence of such a cell type contradicts current ... embryo, and may hold clues to origins of, and future ... from a single cell into a complex being made up ...
Breaking Biology News(10 mins):Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2House fly genome reveals expanded immune system 2Stem cell discovery challenges dogma on how fetus develops; holds insights for liver cancer and reg 2Stem cell discovery challenges dogma on how fetus develops; holds insights for liver cancer and reg 3
... 19, 2012 A new study by scientists on ... in cell culture, a natural compound can virtually eliminate ... defines a novel class of HIV anti-viral drugs endowed ... and chronically infected cells. The HIV/AIDS pandemic continues ...
... , New Rochelle, NY, July 19, 2012To enable greater ... approaches such as co-firing of high percentages of biomass ... A comprehensive review of the strategies currently available and ... presented in I ndustrial Biotechnology , a peer-reviewed journal ...
... The Wyss Institute for Biologically Inspired Engineering at Harvard ... million contract (including option) from the Defense Advanced Research ... helps improve physical endurance for soldiers in the field. ... onset of fatigue, enabling soldiers to walk longer distances, ...
Cached Biology News:Scripps Research scientists show potent new compound virtually eliminates HIV in cell culture 2Strategies to improve renewable energy feedstocks 2Harvard's Wyss Institute to develop smart suit that improves soldiers' physical endurance 2
(Date:10/22/2014)... Cranford, NJ (PRWEB) October 22, 2014 ... technologies and services – has been working with a ... Health and New Haven Farms Community Farming Collaborative to ... iPad-based ePRO solution. The study, which started in ... Wellness program that provides participants weekly exposure to and ...
(Date:10/22/2014)... SC (PRWEB) October 22, 2014 ... of its cardiovascular pharmacogenetics menu, which enables healthcare ... outcomes. With PCLS’s evidence-based results, healthcare providers ... and optimize their therapy, while minimizing risks for ... the U.S., according to the FDA [1] more ...
(Date:10/22/2014)... Oct. 22, 2014 /PRNewswire/ - iCo Therapeutics ("iCo" or ... next steps for its Oral Amphotericin B program.  ... in vitro work involving samples from ... plans to complete pre clinical studies and regulatory ... 1A clinical trial, utilizing approximately $700,000 of funding ...
(Date:10/22/2014)... and HONG KONG , Oct. 22, ... therapeutics enterprise, announced today that rare disease expert ... vice president, research. Dr. McKew brings more than two ... positions at the National Institutes of Health, Wyeth Research ... Wyeth). Dr. McKew will lead aTyr,s efforts to expand and ...
Breaking Biology Technology:myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... difficult transfections when using the,complete control kit ... , We describe the vector pFB-ERV, an MMLV-based ... proteins RXR and VgEcR.,In stable cell lines infected ... induction ratios of greater than 200-fold are attainable ...
... One column purification of protein with native amino ... Wyborski * John C. Bauer * Barbara McGowan ... Systems, Inc. , Stratagene has improved the Affinity ... the pCAL-n-EK vector. This E. coli,cloning and expression ...
... highly specific digestion of purified,proteins or proteins in ... Claycomb * ChaoFeng Zheng * Denise Wyborski,* John ... Bernardino * Peter Vaillancourt ,Stratagene Cloning Systems, Inc. ... that cleaves at the carboxy terminus of its ...
Cached Biology Technology:pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 2pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 3pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 4pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 5pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins 6Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 2Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 3Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 4Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 5Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 6Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 7Expression and Purification of Recombinant Proteins That Have Native Amino,Acid Sequence 8Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 2Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 3Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini 4
... Genome-pULSe Kit delivers a novel procedure for ... labeling of whole genome DNA from small ... provide a quick and highly reproducible amplification ... consists of a REPLI-g Mini Kit module ...
... Introduction of a protein into cells permits ... signaling pathways, apoptotic pathways, and transcription factor-mediated ... studying the effects of a specific protein ... the protein directly into the cell. ProteoJuice ...
... ST-190-XY is an automated instrument with a ... in real-time using light or fluorescence microscopy. ... muscle, and fibroblast cells can be simulated ... X and Y axis of our strain ...
... Our Smart Solutions has now entered the ... (HCS) by partnering up with Cellomics, Inc. ... the integration of Beckman Coulter‘s automated solutions ... deeper biological information at the sub-cellular level. ...
Biology Products: