Navigation Links
CRISPR critters: Scientists identify key enzyme in microbial immune system
Date:9/9/2010

A. Cas proteins will use these matching sequences to bind the crRNA to the invading virus or plasmid and silence it."

Haurwitz says the CRISPR/cas system for silencing foreign DNA in prokaryotes is analogous to the way in which short interfering or siRNAs correct genetic problems in eukaryotes. Over time, the CRISPR/cas system will build up inheritable DNA-encoded immunity from future invasions by the same types of viruses and plasmids.

With their crystal structure model of the Csy4 enzyme bound to its cognate RNA, which features a resolution of 1.8 Angstroms, the Berkeley CRISPR research team has shown that Csy4 makes sequence-specific interactions in the major groove of the CRISPR RNA repeat stem-loop. Together with electrostatic contacts to the phosphate backbone, these interactions enable Csy4 to selectively bind to and cleave pre-crRNAs using phylogenetically conserved residues of the amino acids serine and histidine in the active site.

"Our model explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases," Doudna says.

Doudna and her colleagues produced their 1.8 Angstrom resolution crystallographic structure using the experimental end stations of Beamlines 8.2.1 and 8.3.1 at Berkeley Lab's Advanced Light Source (ALS). Both beamlines are powered by superconducting bending magnets "superbends" and both feature state-of-the-art multiple-wavelength anomalous diffraction (MAD) and macromolecular crystallography (MX) capabilities. Beamline 8.2.1 is part of the suite of protein crystallography beamlines that comprise the Berkeley Center for Structural Biology.

"The ALS and its protein crystallography beamlines continue to be a critical resource for our research, Doudna says.

The crRNAs used by the CRISPR/cas system for the targeted interference of foreign DNA join the growing ranks of small RNA molecules that mediate a variety of processes in both eukaryotes an
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
CRISPR critters: Scientists identify key enzyme in microbial immune system
(Date:7/31/2015)... de julho de 2015 A 10 a. ... será realizada pela BGI de 22-25 de outubro de 2015, ... A conferência está celebrando seu 10 o. aniversário ... se tornou uma das reuniões mais influentes do mundo ... mais dinâmicos, entusiastas e prazerosos. A ...
(Date:7/31/2015)... 2015 La 10 th International Conference on ... BGI del 22 al 25 de octubre de 2015 en ... la conferencia celebra su décimo aniversario. Desde su inauguración en ... las reuniones anuales más influyentes del mundo en el campo ... entusiastas y mejores a nivel científico. ICG-10 ...
(Date:7/31/2015)... --NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... mobile commerce market and creator of the Wocket® smart ... for ELECTRONIC CRYPTO-CURRENCY MANAGEMENT METHOD AND SYSTEM ... crypto-currencies such as Bitcoin into the consumer market of ... manage all payments.  The technology manages ...
Breaking Biology News(10 mins):A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 2La 10th International Conference on Genomics (ICG-10) se llevará a cabo en octubre 3NXT-ID Patents Electronic Crypto-Currency Management Technology 2NXT-ID Patents Electronic Crypto-Currency Management Technology 3NXT-ID Patents Electronic Crypto-Currency Management Technology 4
... ear. Sylvia Plath stuck her head in the oven. History ... ways. Were these artists simply mad or brilliant? According to ... of the Association for Psychological Science, maybe both. In ... Szabolcs Kri of Semmelweis University in Hungary focused his research ...
... first experiences with exotic species in the Basque Country, ... concretely in Laukiz, Lezama and Alonsotegui (Muro, 1975) where ... expansion of rapid growth plantations such as those of ... of the growing paper pulp industry. Blights and diseases ...
... MADISON, WI, SEPTEMBER 28, 2009 -- Emphasizing cross-disciplinary ... mass-volume relationships, can help undergraduates learn real-world, problem-solving ... soil science careers. Drs. Josh Heitman and ... Department, highlighted this need for quantitative measurement skills ...
Cached Biology News:Mad genius: Study suggests link between psychosis and creativity 2Canker disease in eucalyptus in the Basque Country 2Canker disease in eucalyptus in the Basque Country 3Canker disease in eucalyptus in the Basque Country 4Help students think like soil scientists 2Help students think like soil scientists 3
(Date:8/28/2015)... ... August 28, 2015 , ... Riordan-McKenna Institute ... DO, announced today that the use of sterile, dehydrated amniotic tissue AlphaPATCH™ developed ... an otherwise non-healing surgical knee wound. , The case involved a 78-year-old male, ...
(Date:8/27/2015)... ... 27, 2015 , ... The Kansas City Animal Health Investment ... health companies to hear from animal health companies with the newest technology. Companies ... agreements or distribution contracts. This meeting is one of the only investment ...
(Date:8/27/2015)... Mich. , Aug. 27, 2015  Neogen Corporation ... acquired the stock of United Kingdom ... of microbiological culture media and diagnostic systems. ... grown into a leading provider of microbial testing and ... clinical testing markets. The company currently sells into more ...
(Date:8/27/2015)... CELEBRATION, Fla. , Aug. 27, 2015 ... announced that a patient,s eyes—and life—were saved by doctors ... , working with NNFF, NovaBay Pharmaceuticals and Dr. ... Wound Care Center at Seton Medical Center in ... The successful treatment used a breakthrough approach, pioneered by ...
Breaking Biology Technology:Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 2VetStem Biopharma, Inc. Among Seventeen Companies Selected to Present at the KC Animal Health Investment Forum on September 1st 2015 3Neogen acquires U.K.-based Lab M Holdings 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3
... N.J., Oct. 6 NEXCORE Technology, Inc., a,world-class ... an agreement with GI Supply of Camp Hill, ... Wand. Polar Wand is a portable,device that uses ... the gastrointestinal tract in patients resistant to conventional,endoscopic ...
... Environmental,Tectonics Corporation,s (Amex: ETC ) ("ETC" or ... of two (2) BARA-MED(R) XD,Monoplace Hyperbaric Chambers to ... delighted to work with Saint Barnabas Medical Center, ... one of the largest health care providers in ...
... BioMed Realty Trust, Inc.,(NYSE: BMR ) today ... its over-allotment option in full to purchase an ... BioMed,s previously,announced offering that priced on October 1, ... common stock, raising net proceeds of,approximately $212.4 million ...
Cached Biology Technology:NEXCORE Technology Selected to Manufacture New Endoscopic Cryotherapy Device 2Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 2Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 3Environmental Tectonics Corporation Announces the Placement of Two Monoplace Hyperbaric Chambers at Saint Barnabas Medical Center in New Jersey 4BioMed Realty Trust Announces Exercise of Over-Allotment Option 2BioMed Realty Trust Announces Exercise of Over-Allotment Option 3
... the first biological reagent that ... and not just by inhibiting ... anti-mycoplasma agent that can be ... directly. The cytotoxicity of Mynox ...
A component of the Acridinium protein labeling kit....
... can be used to label ... covalently bound acridinium NHS ester ... presence of hydrogen peroxide. Acridinium ... used as a detection method ...
... Guava is making flow cytometry accessible to anyone ... appreciate the latest addition to Guavas on-demand microcapillary ... easy to use, surprisingly affordable, very compact, ... 3-color detection. The Guava EasyCyte Mini has all ...
Biology Products: