Navigation Links
CRISPR critters: Scientists identify key enzyme in microbial immune system
Date:9/9/2010

the RNA recognition mechanisms in Cys4 and Dicer, the enzyme that plays a critical role in eukaryotic RNA interference."

Doudna is a leading authority on RNA molecular structures who holds joint appointments with Berkeley Lab's Physical Biosciences Division and UC Berkeley's Department of Molecular and Cell Biology and Department of Chemistry. She is also an investigator with the Howard Hughes Medical Institute (HHMI). The results of this latest research on CRISPR are reported in the journal Science in a paper titled "Sequence- and structure-specific RNA processing by a CRISPR endonuclease." Co-authoring the paper with Doudna were Rachel Haurwitz, Martin Jinek, Blake Wiedenheft and Kaihong Zhou.

CRISPR is a unit of DNA, usually on a microbe's chromosome, made up of "repeat" elements, base-pair sequences ranging from 30 to 60 nucleotides in length, separated by "spacer" elements, variable sequences that are also from 30 to 60 nucleotides in length. CRISPR units are found in about 40-percent of all bacteria whose genomes have been sequenced, and about 90-percent of archaea. A microbe might have several CRISPR loci within its genome and each locus might contain between four and 100 CRISPR repeat-spacer units. Doudna and her colleagues studied CRISPR in Pseudomonas aeruginosa, a common bacterium that is ubiquitous in the environment.

Rachel Haurwitz, a graduate student in Doudna's research group and the first author on the Science paper, explains how the CRISPR/Cas immunity system works.

"When a bacterium recognizes that it has been invaded by a virus or a plasmid, it incorporates a small piece of the foreign DNA into one of its CRISPR units as a new spacer sequence. The CRISPR unit is then transcribed as a long RNA segment called the pre-crRNA. The Csy4 enzyme cleaves this pre-crRNA within each repeat element to create crRNAs about 60 nucleotides long that will contain sequences which match portions of the foreign DN
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
CRISPR critters: Scientists identify key enzyme in microbial immune system
(Date:9/30/2014)... (LMU) in Munich report that a new class ... to chemotherapeutic drugs. They have also pinpointed the ... for anti-tumor agents. , Researchers led by ... of the Technische Universitt Mnchen have identified a ... weapon in the fight against malignant tumors. The ...
(Date:9/30/2014)... a suite of perilous threats in today,s ocean. ... climate change, fragile coral ecosystems are disappearing at ... some species of corals surrounding the island of ... in their tropical environment: coral guard-crabs. New research ... Marine Station scientist Seabird McKeon and the museum,s ...
(Date:9/29/2014)... DC September 29, 2014 -- Plants have ... bacteria help the pants extract nutrients and defend ... pathogens from contaminating fruits and vegetables. Now, scientist ... bacteria inside of seeds; thus ensuring that sprouting ... from the University of Notre Dame, presented their ...
Breaking Biology News(10 mins):Cancer therapy: Driving cancer cells to suicide 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Plants prepackage beneficial microbes in their seeds 2
... of their woody material, could be the key to a ... who add that the approach could also help turn agricultural ... component of woody plant material, is woven in with cellulose ... of wind and microbial attack. However, this protective barrier or ...
... NEW YORK, Dec. 22, 2008 NYU Langone Medical Center ... La Jolla, Calif., have discovered an unexpected cause for the ... of the central nervous system, according to a study published ... new way of thinking about how the human immune system ...
... for humans, Plasmodium falciparum , has been linked ... fairly isolated on the malarial family treeuntil now. A ... Genomics at the American Museum of Natural History reveals ... close evolutionary relationship with P. falciparum and ...
Cached Biology News:Modified plants may yield more biofuel 2Peering inside the skull of a mouse to solve meningitis mystery 2Peering inside the skull of a mouse to solve meningitis mystery 3African thicket rat malaria linked to virulent human form 2
(Date:9/30/2014)... MAR, Calif. , Sept. 30, 2014 ... for women,s health announces its receipt of a patent ... FemmyCycle menstrual cup has also received FDA clearance in ... Canada, and the CE in Europe. This patent is related ... conjunction with this issuance, the company is releasing FemmyCycle ...
(Date:9/30/2014)... September 30, 2014 SoundConnect, ... collaboration company is dedicated to supporting business communication ... Julie Power, a carrier services ... collaboration solutions stays connected and productive at all ... increased movement of businesses to cloud communications services. ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 Back in the ... 500 square feet. per employee, which included space for circulation ... then.” says Jeff Howell, partner at Nidea Corporate Real ... in Toronto. “By the year 2000, however, 250 square ... seeing that number come down to 175 to 225 square ...
(Date:9/29/2014)... , Sept. 29, 2014  Ten of ... fields of cartilage repair, regeneration, allograft reconstruction and ... around the world gathered to discuss the future ... Institute of Regenerative Medicine symposium, Cartilage Regeneration: ... Topics included Orthopedic Biosurgery, Minced Juvenile Allograft, and ...
Breaking Biology Technology:The FemmyCycle 2SoundConnect Expands Carrier Services Division 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 2ITRA Global Reports on How Companies Are Squeezing More Employees into Less Office Space 3World Renowned Experts Discuss Future Of Cartilage Regeneration 2World Renowned Experts Discuss Future Of Cartilage Regeneration 3
... LOUIS, Aug. 27 Missouri Coalition for,Lifesaving Cures ... far-,reaching initiative petition filed last week by opponents ... worded and scientifically unsound that it,would exclude Missourians ... syndromes from its definition of "human." In ...
... Drug Royalty LP 2 (DRC) announced,today the ... worldwide,royalty interest in PEG-INTRON for $92.5M. DRC will ... certain royalty recognition levels be met.,PEG-INTRON (peginterferon alpha-2b), ... is marketed worldwide by Schering-Plough Corporation (SGP). ...
... 27 NovaQuest has,made a strategic investment ... specializing in respiratory,disorders. The investment was part ... million) in the Montreal-based company. Last ... partnering group,of Quintiles Transnational, helps TOPIGEN accelerate ...
Cached Biology Technology:New Initiative Has Shocking Effect: Thousands of Missourians with Abnormal Chromosome Structures Are Not Human 2Drug Royalty Corporation (DRC) Announces Acquisition Regarding PEG-INTRON(R) From Enzon Pharmaceuticals, Inc. (ENZN) 2NovaQuest Advances 'Virtual Development' Model With Strategic Investment in TOPIGEN Pharmaceuticals 2
...
... reagent; Ideal for clarifying many items ... nucleic acids, & proteoglycans; Contains no ... downstream processing; Utilizes a reagent that ... as a suspension in saline; Simple ...
A simple, sensitive and reliable method for determining free sulfhydryl content in peptides, protein...
... Tecans open platform for clinical diagnostic applications, ... performance in the clinical diagnostic and blood ... barcode reading and sample traceability as standard ... complies with the IVD-Directive* established in the ...
Biology Products: