Navigation Links
Bacterial reporters that get the scoop
Date:3/17/2014

ling dogma in synthetic biology, which is to design genetic systems that drive cell behavior from scratch, said Wyss Institute Senior Staff Scientist Jeff Way, Ph.D., a coauthor on the paper. On the other hand, "Nature has a tried-and-true blueprint for memory systems if you know where to look," Way said. "Why not just accept Nature as it is, and develop the system from there?"

The genetic switch the team inserted in E. coli came from lambda phage, a virus that commonly attacks this bacterium.

After invading E. coli, lambda typically lays low, living in a stealth mode called lysogeny in which its DNA simply hangs out in the E. coli's genome. But when the bacterium's DNA is damaged and only then the switch flips, instructing the virus to enter a mode called lysis in which it multiplies inside the cell and breaks through its membrane in a kind of microbial explosion.

"This is a very stable system in Nature," said lead author Jonathan Kotula, Ph.D., a Postdoctoral Fellow at HMS who is also affiliated with the Wyss Institute. "We knew the lambda switch would be a great candidate for the memory element, and we simply tweaked it to meet our needs."

The cells with the engineered lambda switch would not become lytic under any conditions. Kotula and the rest of Silver's team used standard molecular genetic tools to rig the switch such that it turned on only in the presence of an inactive form of the antibiotic tetracycline.

In laboratory experiments, the switch turned on within a few hours of exposure to the antibiotic and stayed in this 'ON' state inside E. coli for a week or more, even as the bacteria grew and divided. In short, the cells "remembered" that they had seen that molecule in the gut.

"It was truly shocking how cleanly the experiments worked," said Jordan Kerns, Ph.D., a Wyss Institute Postdoctoral Fellow.

But to function as a living diagnostic, the engineered
'/>"/>

Contact: Kristen Kusek
kristen.kusek@wyss.harvard.edu
617-432-8266
Wyss Institute for Biologically Inspired Engineering at Harvard
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Spread of antibiotic resistance understood by unravelling bacterial secretion system
2. Bacterial fibers critical to human and avian infection
3. £4 million to tackle spread of bacterial infections
4. Puzzling question in bacterial immune system answered
5. Little but lethal -- small RNAs coordinate bacterial attack on epithelial cells
6. Gut microbes affect MicroRNA response to bacterial infection
7. In surprise finding, blood clots absorb bacterial toxin
8. New method of DNA editing allows synthetic biologists to unlock secrets of a bacterial genome
9. Structure of bacterial nanowire protein hints at secrets of conduction
10. Evidence of 3.5 billion-year-old bacterial ecosystems found in Australia
11. Changing the conversation -- polymers disrupt bacterial communication
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Bacterial reporters that get the scoop
(Date:2/5/2015)... , Jan. 28, 2015 Research and Markets ... addition of the "Global Biometrics Market (2014-2020): ... and Countries " report to their offering. ... Asia-Pacific is anticipated to overtake ... owing to increasing government spending towards IT security, ...
(Date:1/22/2015)... ( http://www.researchandmarkets.com/research/4trb7t/biometrics_a ) has announced the addition of ... to their offering. , The primary goal ... who she/he is claiming to be, and for this purpose, ... characteristics, such as fingerprint, hand or palm print, iris, face ...
(Date:1/22/2015)... , Jan. 13, 2015  Today, FreeWavz ( ... launched its crowdfunding campaign on Fundable, https://www.fundable.com/freewavz ... build of production capacity to meet customer demand. ... Photo - http://photos.prnewswire.com/prnh/20150113/168790 ...
Breaking Biology News(10 mins):Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 2Global Biometrics Market (2014-2020): Market Forecast By Technologies, Applications, End Use, Regions and Countries 3Biometrics - A Global Market Overview 2Biometrics - A Global Market Overview 3FreeWavz Launches on Fundable to Drive Speed-to-Market in 2015 2
... This press release is available in Spanish . ... real headache. U.S. Department of Agriculture (USDA) scientists have identified ... and offer a potential bio-based approach to controlling them. Other ... termite, a nonnative species from Asia, feeds on cellulose from ...
... shown that 30 minutes of daily training provide an equally effective ... have just been published in the American Journal of Physiology ... thirteen weeks, a research team at the Faculty of Medical and ... in their efforts to get into better shape. Half of the ...
... BOSTON, Aug. 22, 2012 Evermedia Group, Inc. (OTC: EVRM) ... 2012 revenue of $703,620, an increase of more than 375% ... all revenue generated in fiscal year 2011 as represented by ... increase in revenue is largely attributable to revenue generated by ...
Cached Biology News:Nematodes with pest-fighting potential identified 230 minutes of daily exercise does the trick 2Evermedia Group Announces 375% Increase in Revenue for Second Quarter 2012 2Evermedia Group Announces 375% Increase in Revenue for Second Quarter 2012 3
(Date:3/3/2015)... AtheroNova Inc. (OTCQB: AHRO) today announced that the Company and ... 11 of the U.S. Bankruptcy Code in the U.S. Bankruptcy ... Santa Ana division (the "Bankruptcy Court") on ... to be jointly administered. The Company will evaluate ... for all or substantially all of its assets to a ...
(Date:3/3/2015)... Cambridge Semantics, the leading provider of smart ... that it has been named to KMWorld’s 2015 list ... , To create the list each year, KMWorld ... integrators who identify organizations dedicated to the true value ... the right people at the right time. , ...
(Date:3/3/2015)...  Johnson & Johnson Innovation LLC today announced the ... (JLABS @SSF), a 30,000-square foot incubator located at ... Calif. The Johnson & Johnson Innovation, JLABS ... across the healthcare spectrum including biotech, pharmaceutical, medical device, ... resident startups have been selected, including the winners of ...
(Date:3/3/2015)... 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ... announced it has initiated commissioning activities for its 30,000 ... Sarnia, Ontario , Canada.  Commissioning ... with the facility being in commercial operation in Q3 ... two months and it is carrying out commissioning and ...
Breaking Biology Technology:AtheroNova and its Subsidiary File for Chapter 11 Bankruptcy Protection 2AtheroNova and its Subsidiary File for Chapter 11 Bankruptcy Protection 3AtheroNova and its Subsidiary File for Chapter 11 Bankruptcy Protection 4Cambridge Semantics Named to KMWorld’s 2015 ‘100 Companies That Matter in Knowledge Management’ 2Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 2Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 3Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 4Johnson & Johnson Innovation Opens JLABS @South San Francisco and Announces First Five Resident Startups 5BioAmber Begins Commissioning of Sarnia Commercial Plant 2BioAmber Begins Commissioning of Sarnia Commercial Plant 3
... GmbH and BASF developed pro-t-action(TM) product based on probiotic ... oral careFull research studies introduced at International Association of ... FL , April 1-4 LUDWIGSHAFEN, Germany and ... remedy a main cause of tooth decay affecting an ...
... Board: SNKTY), a Life Sciences company engaged in the ... today announced plans for the creation of a new communication ... updated on key events. Bi-weekly updates will be recorded on ... and running in early May. In addition, callers will be ...
... Neuroscience paper describes role of Zinc in developing Alzheimer,s ... PRAN ) today announced that an independent ... California, Irvine , validates Prana,s fundamental drug strategy for ... and brain metals, such as zinc, that damages synapses ...
Cached Biology Technology:BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 2BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 3BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 4BASF Set To Commercialize pro-t-action(TM) Eliminating Caries Causing Bacteria From the Mouth 5Senetek PLC Announces New Investor Communication Hotline 2Independent Study Corroborates Prana's Strategy to Treat Alzheimer's Disease Patients. 2Independent Study Corroborates Prana's Strategy to Treat Alzheimer's Disease Patients. 3Independent Study Corroborates Prana's Strategy to Treat Alzheimer's Disease Patients. 4
Peptide Sequence: ovine COX-1 amino acids 272-282 (LMHYPRGIPPQ) To be used in conjunction with Cayman's COX-1 (ovine) polyclonal antiserum (Catalog No. 160108) to block protein-antibody complex f...
Laminin beta-1/gamma-1 (612C10)...
... choice of Printing Surface (3 glass slides coated with ... 1 silicon wafer coated with 1000 Angstroms of Gold ... PDMS Stamps (3 PDMS micropatterned stamps with series of ... 10 mm or 200 um x 10 mm lines, ...
X box-binding protein 1...
Biology Products: