Navigation Links
Bacterial reporters that get the scoop
Date:3/17/2014

ling dogma in synthetic biology, which is to design genetic systems that drive cell behavior from scratch, said Wyss Institute Senior Staff Scientist Jeff Way, Ph.D., a coauthor on the paper. On the other hand, "Nature has a tried-and-true blueprint for memory systems if you know where to look," Way said. "Why not just accept Nature as it is, and develop the system from there?"

The genetic switch the team inserted in E. coli came from lambda phage, a virus that commonly attacks this bacterium.

After invading E. coli, lambda typically lays low, living in a stealth mode called lysogeny in which its DNA simply hangs out in the E. coli's genome. But when the bacterium's DNA is damaged and only then the switch flips, instructing the virus to enter a mode called lysis in which it multiplies inside the cell and breaks through its membrane in a kind of microbial explosion.

"This is a very stable system in Nature," said lead author Jonathan Kotula, Ph.D., a Postdoctoral Fellow at HMS who is also affiliated with the Wyss Institute. "We knew the lambda switch would be a great candidate for the memory element, and we simply tweaked it to meet our needs."

The cells with the engineered lambda switch would not become lytic under any conditions. Kotula and the rest of Silver's team used standard molecular genetic tools to rig the switch such that it turned on only in the presence of an inactive form of the antibiotic tetracycline.

In laboratory experiments, the switch turned on within a few hours of exposure to the antibiotic and stayed in this 'ON' state inside E. coli for a week or more, even as the bacteria grew and divided. In short, the cells "remembered" that they had seen that molecule in the gut.

"It was truly shocking how cleanly the experiments worked," said Jordan Kerns, Ph.D., a Wyss Institute Postdoctoral Fellow.

But to function as a living diagnostic, the engineered
'/>"/>

Contact: Kristen Kusek
kristen.kusek@wyss.harvard.edu
617-432-8266
Wyss Institute for Biologically Inspired Engineering at Harvard
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Spread of antibiotic resistance understood by unravelling bacterial secretion system
2. Bacterial fibers critical to human and avian infection
3. £4 million to tackle spread of bacterial infections
4. Puzzling question in bacterial immune system answered
5. Little but lethal -- small RNAs coordinate bacterial attack on epithelial cells
6. Gut microbes affect MicroRNA response to bacterial infection
7. In surprise finding, blood clots absorb bacterial toxin
8. New method of DNA editing allows synthetic biologists to unlock secrets of a bacterial genome
9. Structure of bacterial nanowire protein hints at secrets of conduction
10. Evidence of 3.5 billion-year-old bacterial ecosystems found in Australia
11. Changing the conversation -- polymers disrupt bacterial communication
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Bacterial reporters that get the scoop
(Date:11/4/2014)... Fla. (November 4, 2014) — Think about the way ... How do neighboring cells know that they are supposed ... how do these tissues find the correct place and ... answering these crucial questions. , In a new study, ... to communicate with their surrounding neighbors, at the head-trunk ...
(Date:11/4/2014)... Lithuania , Nov. 4, 2014   ... technologies, today announced that the latest version of ... in the Ongoing MINEX evaluation organized ... of fingerprint algorithms using the INCITS 378 fingerprint ... mandatory requirement in public tenders in ...
(Date:11/3/2014)... that low birth weight and preterm birth are linked ... adulthood. Findings published in the American College of Rheumatology ... that low birth weight and pre-term babies were not ... as adults. , According to the ACR, 27 ... with clinical OA. Symptoms of OA range from mild ...
Breaking Biology News(10 mins):The inside story: How the brain and skull stay together 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 3Preterm, low birth-weight babies may need new hips in adulthood 2
... world in which polymer membranes with molecular-sized ... fuels, or desalinating sea water, among many other ... membranes can be readily fabricated in bulk quantities. ... that road has now been successfully demonstrated. ...
... presented in the thesis has examined children, adolescents and young ... with and without asthma. The first study revealed that 3-,year-olds ... asthma. "The children with asthma had a greater tendency to ... and were therefore given sugary drinks more often. This may ...
... of the challenges of gene therapy - a set of ... RNA) - is to assure that this material arrives directly ... amount along the way and without producing any undesired side ... of different types of vectors, molecules capable of transporting genetic ...
Cached Biology News:Polymer membranes with molecular-sized channels that assemble themselves 2Polymer membranes with molecular-sized channels that assemble themselves 3Young people with asthma run a greater risk of developing caries 2Nanodisk gene therapy 2
(Date:11/21/2014)... , Todd Martensen ... Mariano Rodríguez es elegid vicepresidente senior de desarrollo empresarial ... en marcha para comenzar de forma rápida la comercialización de ... aprobación en Europa   , KLOX ... los siguientes nombramientos: Todd Martensen ha ...
(Date:11/21/2014)... Palo Alto, Calif. (PRWEB) November 20, 2014 ... (CHI), a nonprofit, public policy research organization representing ... and pharmaceutical firms, and PwC US ... other states in life sciences industry growth. The ... and 2013. The 2015 California Biomedical Industry ...
(Date:11/21/2014)... 21, 2014 2014 Deep Research ... is a professional and in-depth research report on ... information, like its definition, classification, application, and ... This report covers the international market analysis, including ... as the US, Europe, Asia, China, Japan etc. ...
(Date:11/21/2014)... /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ("Biorem" or "the Company") today announced ... 30, 2014.  Biorem,s complete 2014 third quarter financial statements and MD&A ... Summary:Three-months ended September 30, , Nine-months ended ... 2014 , 2013 , 2014 ... , 6,715 , 14,476 Gross profit , ...
Breaking Biology Technology:KLOX Technologies anuncia sus nombramientos ejecutivos 2KLOX Technologies anuncia sus nombramientos ejecutivos 3KLOX Technologies anuncia sus nombramientos ejecutivos 4KLOX Technologies anuncia sus nombramientos ejecutivos 5California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 2California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 4Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 2Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 3Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3
... Post-Its, a text file on my laptop, whiteboards, notepads or whatever ... out a free online service, Ta-Da Lists , that provides ... It was made by 37 Signals , a Web development ... offer it free without any advertising. , ,(Mostly, it's promoting their ...
... the state has dozens of them operating but ... state grants to inject fresh life into a tried-and-true ... 26 grants totaling more than $750,000 from the state's ... Grant (CDBG) programs. Many of the grant winners are ...
... biotech researchers, including stem-cell pioneer James Thomson, have ... new drug screening company in Madison's University ... II Ventures LP, a venture capital firm that ... Thomson, Craig January and Timothy Kamp have started ...
Cached Biology Technology:Ta-Da Lists makes it fun to check things off 2Grant winners hope to cultivate job-creating tech businesses 2Grant winners hope to cultivate job-creating tech businesses 3Cellular Dynamics hits market with UW research heavyweights 2
... IDS 25-Hydroxy Vitamin D kit is ... determination of 25-hydroxyvitamin D (25-OH D) ... serum or plasma. Our patented DIRECT ... solvent precipitation and centrifugation and therefore ...
... is a complete assay system for the ... human serum or plasma. The assay incorporates ... the IDS 1,25D RIA followed by quantitation ... ready to use reagents, no organic or ...
Rabbit polyclonal to Nck alpha ( Abpromise for all tested applications). entrezGeneID: 4690 SwissProtID: P16333...
anti-achaete...
Biology Products: