Navigation Links
BGI develops RNA-Seq (Quantification) from as low as 100 Ng total RNA
Date:9/20/2011

September 20th, 2011,Shenzhen, China BGI, the world's largest genomics organization, reported that they have achieved optimization RNA-Seq (Quantification) library construction with total RNA inputs as low as 100 ng. This breakthrough enables the application of RNA-Seq (Quantification) technology to experimental designs utilizing samples derived from small numbers of cells, such as those widely used in pharmaceutical research, cancer research, and immunology.

RNA-Seq (Quantification), a version of Next Generation Sequencing (NGS), is used for transcriptome quantification and analyzing the gene expression of certain biological objects in specific conditions. It can be widely applied in biomarker detection, basic medical research, drug discovery, among others. Compared with microarray technology, high-throughput RNA sequencing can provide comprehensive assessment of RNA expression profiles with the advantages of high-throughput data, low background, high sensitivity and repeatability.

However, some tissues or cultures from specialized cells involved in clinical and pharmaceutical research make it difficult to obtain sufficient RNA for RNA-Seq (Quantification), which previously required 1 μg or more total RNA. BGI has optimized the procedures to enable RNA-Seq (Quantification) using as little as 100 ng total RNA sample input to generate high-quality data. "The improvement of RNA-Seq (Quantification) not only can simplify sample preparation, but also make this sequencing service more cost-effective and with rapid turnaround time," said Jiong Zhang, Technical Specialist at BGI.

To ensure the accuracy and quality of data, many evaluations have been conducted at BGI, including the reads quality, assessments of reads randomness, gene coverage, experimental reproducibility, and data accuracy. Results demonstrated that the data generated from 100 ng sample input library was as high-quality as that from traditional 1 μg RNA input library. "I hope our enhanced technique can contribute more to drug discovery and therapeutic application in the future," added Zhang.


'/>"/>

Contact: Liu Jia
liujia@genomics.cn
Beijing Genomics Institute
Source:Eurekalert

Related biology news :

1. UConn scientist develops sterile variety of invasive plant
2. BGI develops new strategy to uncover structural variations of human genomes
3. How muscle develops: A dance of cellular skeletons
4. New research provides insight into how OCD develops
5. UBC-led team develops platform to monitor hematopoietic stem cells
6. NJIT professor develops a biologically inspired catalyst, an active yet inert material
7. NC State develops material to remove radioactive contaminants from drinking water
8. Advertising Research Foundation Develops Standards for Neuromarketing Research
9. Syracuse University team develops functionally graded shape memory polymers
10. WebLib develops application for Elseviers SciVerse applications
11. Researcher develops accurate method for detecting dangerous fluoride
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2017)... , May 16, 2017   Bridge Patient ... organizations, and MD EMR Systems , an ... partner for GE, have established a partnership to ... product and the GE Centricity™ products, including Centricity ... These new integrations will allow ...
(Date:4/17/2017)... MELBOURNE, Florida , April 17, 2017 ... security technology company, announces the filing of its 2016 Annual Report ... Securities and Exchange Commission. ... Report on Form 10-K is available in the Investor Relations section ... well as on the SEC,s website at http://www.sec.gov . ...
(Date:4/11/2017)... April 11, 2017 No two people ... at the New York University Tandon School of ... have found that partial similarities between prints are ... in mobile phones and other electronic devices can ... The vulnerability lies in the fact that fingerprint-based ...
Breaking Biology News(10 mins):
(Date:6/27/2017)... ... June 27, 2017 , ... Brain State Technologies, a leading ... on June 15th to fund production of the new B2v2 wearable brainwave balancing ... goal by more than 150% in a little over a week. , Utilizing ...
(Date:6/27/2017)... (PRWEB) , ... June 27, 2017 , ... ... Certification Program (ESTCP), powder activated carbon (PAC)-based materials do not have negative short- ... community at a site contaminated with polychlorinated biphenyls (PCBs) located at the Puget ...
(Date:6/27/2017)... ... June 27, 2017 , ... DuPont Pioneer (DuPont) and ERS ... the ERS patent portfolio covering CRISPR-Cas genome editing technology for all agricultural uses ... (IP) of the CRISPR-Cas technology from co-inventor and co-owner Emmanuelle Charpentier, Ph.D. ...
(Date:6/26/2017)... (PRWEB) , ... June 26, 2017 , ... Third Wave ... role of Sales Director, focused on leading new business development and ensuring quality customer ... of experience in the food ingredient industry in technical, marketing and sales roles. “Brett’s ...
Breaking Biology Technology: