Navigation Links
BGI develops RNA-Seq (Quantification) from as low as 100 Ng total RNA
Date:9/20/2011

September 20th, 2011,Shenzhen, China BGI, the world's largest genomics organization, reported that they have achieved optimization RNA-Seq (Quantification) library construction with total RNA inputs as low as 100 ng. This breakthrough enables the application of RNA-Seq (Quantification) technology to experimental designs utilizing samples derived from small numbers of cells, such as those widely used in pharmaceutical research, cancer research, and immunology.

RNA-Seq (Quantification), a version of Next Generation Sequencing (NGS), is used for transcriptome quantification and analyzing the gene expression of certain biological objects in specific conditions. It can be widely applied in biomarker detection, basic medical research, drug discovery, among others. Compared with microarray technology, high-throughput RNA sequencing can provide comprehensive assessment of RNA expression profiles with the advantages of high-throughput data, low background, high sensitivity and repeatability.

However, some tissues or cultures from specialized cells involved in clinical and pharmaceutical research make it difficult to obtain sufficient RNA for RNA-Seq (Quantification), which previously required 1 μg or more total RNA. BGI has optimized the procedures to enable RNA-Seq (Quantification) using as little as 100 ng total RNA sample input to generate high-quality data. "The improvement of RNA-Seq (Quantification) not only can simplify sample preparation, but also make this sequencing service more cost-effective and with rapid turnaround time," said Jiong Zhang, Technical Specialist at BGI.

To ensure the accuracy and quality of data, many evaluations have been conducted at BGI, including the reads quality, assessments of reads randomness, gene coverage, experimental reproducibility, and data accuracy. Results demonstrated that the data generated from 100 ng sample input library was as high-quality as that from traditional 1 μg RNA input library. "I hope our enhanced technique can contribute more to drug discovery and therapeutic application in the future," added Zhang.


'/>"/>

Contact: Liu Jia
liujia@genomics.cn
Beijing Genomics Institute
Source:Eurekalert

Related biology news :

1. UConn scientist develops sterile variety of invasive plant
2. BGI develops new strategy to uncover structural variations of human genomes
3. How muscle develops: A dance of cellular skeletons
4. New research provides insight into how OCD develops
5. UBC-led team develops platform to monitor hematopoietic stem cells
6. NJIT professor develops a biologically inspired catalyst, an active yet inert material
7. NC State develops material to remove radioactive contaminants from drinking water
8. Advertising Research Foundation Develops Standards for Neuromarketing Research
9. Syracuse University team develops functionally graded shape memory polymers
10. WebLib develops application for Elseviers SciVerse applications
11. Researcher develops accurate method for detecting dangerous fluoride
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/4/2017)... April 4, 2017   EyeLock LLC , a ... the United States Patent and Trademark Office (USPTO) has ... the linking of an iris image with a face ... represents the company,s 45 th issued patent. ... very timely given the multi-modal biometric capabilities that have ...
(Date:3/30/2017)... The research team of The Hong Kong ... identification by adopting ground breaking 3D fingerprint minutiae recovery and matching ... and accuracy for use in identification, crime investigation, immigration control, security ... ... A research team led by ...
(Date:3/28/2017)... India , March 28, 2017 ... IP, Biometrics), Hardware (Camera, Monitors, Servers, Storage Devices), Software ... Vertical, and Region - Global Forecast to 2022", published ... Billion in 2016 and is projected to reach USD ... between 2017 and 2022. The base year considered for ...
Breaking Biology News(10 mins):
(Date:4/19/2017)... ... ... Alisa Wright, founder and CEO of Singota Solutions , has received ... Lafayette, Indiana. , The Distinguished Alumni Award was established in 1984 to honor ... endeavors. , Wright began her career in the pharmaceutical industry with firms such ...
(Date:4/19/2017)... ... 19, 2017 , ... ThermaGenix, the PCR Improvement Company, announces ... several other early achievements at ThermaGenix, including the business formation and licensing agreements, ... will use proceeds from the Series A-1 round to:, , ...
(Date:4/19/2017)... and PUNE, India , April 19, 2017 ... Market: Global Opportunity Analysis and Industry Forecast, 2014-2022 ," the global market was ... by 2022, registering a CAGR of 9.6% from 2016 to 2022. ... ... ...
(Date:4/19/2017)... FRANCISCO, Calif. , April 19, 2017 /PRNewswire/ ... VCYT ) today announced that it will report ... of market on Wednesday, May 3, 2017. Following the ... webcast at 4:30 p.m. Eastern Time to discuss the company,s ... live webcast and subsequent replay may be accessed ...
Breaking Biology Technology: