Navigation Links
Ant guts could pave the way for better drugs
Date:3/25/2008

Scientists have discovered two key proteins that guide one of the two groups of pathogenic bacteria to make their hardy outer shells -- their defense against the world.

The work, they said, could allow researchers to create new antibiotics against gram-negative bacteria, like E. coli and salmonella, that would destroy these bacteria by disabling the mechanism that produces their protective coating.

"A long-term goal is to find inhibitors of these proteins we have discovered," said Natividad Ruiz, a research molecular biologist at Princeton University and the lead author on the paper describing the work. "Small molecule inhibitors could become antibiotics that subvert the outer membrane."

The research, conducted by Ruiz, Thomas Silhavy, Princeton's Warner-Lambert Parke-Davis Professor of Molecular Biology, and others from Harvard University, is described in the online edition of the April 8 Proceedings of the National Academy of Sciences.

The team discovered the proteins through an extended process of elimination. The scientists looked at microbes in the guts of carpenter ants. The bacteria, which have lived there for millions of years -- passed on over many generations -- have lost many of the traits necessary for survival in the outer world. As a result, their collection of genes, known as a genome, is far smaller and simpler than the genome of E. coli.

Scientists sequenced the genome of the model bacterium E. coli 11 years ago, yet they still do not understand the functions of about 40 percent of the thousands of proteins produced by those genes, according to Ruiz. Proteins are the workhorses of cells, directing and producing the creation of many key cell structures and functions.

In contrast, the genome of the bacteria found in the ant gut, Blochmannia floridanus, contains the instructions for only 583 proteins. Since the bacteria are closely related, nearly all of Blochmannia's genes -- 564 -- are found in E. coli. The scientists reasoned that they could find the protein containing the instructions for building the germ's outer casing.

"We designed a computer-based search that filtered out proteins that lacked the characteristics essential for outer membrane construction," Ruiz said. "In the end, only two of the 564 proteins remained."

They found the two missing proteins of a pathway that ferries one of the key components of the outer shell, called LPS, to the cell surface.

Members of Silhavy's laboratory use E. coli as a model system to better understand the workings of the cell, such as how it senses changes in its environment. Silhavy is a bacterial geneticist who has made fundamental contributions to the field of cell biology.


'/>"/>

Contact: Kitta MacPherson
kittamac@princeton.edu
609-258-5729
Princeton University
Source:Eurekalert

Related biology news :

1. Newly created cancer stem cells could aid breast cancer research
2. Obesity and lack of exercise could enhance the risk of pancreatic cancer
3. Finding that 1-in-a-billion that could lead to disease
4. 60 second test could help early diagnosis of common brain diseases
5. Auto immune response creates barrier to fertility; could be a step in speciation
6. Paracetamol, one of most used analgesics, could slow down bone growth
7. Drug could improve pregnancy outcomes in wider range of women with insulin resistance
8. Thousands of starving children could be restored to health with peanut butter program
9. Nanotech could make solar energy as easy and cheap as growing grass
10. CO2 emissions could violate EPA ocean-quality standards within decades
11. MIT model could improve some drugs effectiveness
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/30/2017)... -- On April 6-7, 2017, Sequencing.com will host the world,s ... at Microsoft,s headquarters in Redmond, Washington ... health and wellness apps that provide a unique, personalized ... is the first hackathon for personal genomics and the ... the genomics, tech and health industries are sending teams ...
(Date:3/30/2017)... YORK , March 30, 2017 Trends, ... type (physiological and behavioral), by technology (fingerprint, AFIS, iris ... voice recognition, and others), by end use industry (government ... and immigration, financial and banking, and others), and by ... Europe , Asia Pacific , ...
(Date:3/28/2017)... India , March 28, 2017 ... IP, Biometrics), Hardware (Camera, Monitors, Servers, Storage Devices), Software ... Vertical, and Region - Global Forecast to 2022", published ... Billion in 2016 and is projected to reach USD ... between 2017 and 2022. The base year considered for ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... , ... October 10, 2017 , ... ... Center’s FirstHand program has won a US2020 STEM Mentoring Award. Representatives of the ... for Excellence in Volunteer Experience from US2020. , US2020’s mission is to change ...
(Date:10/10/2017)... CRUZ, Calif. , Oct. 10, 2017 ... grant from the NIH to develop RealSeq®-SC (Single Cell), ... kit for profiling small RNAs (including microRNAs) from single ... Analysis Program highlights the need to accelerate development of ... "New techniques for measuring ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
(Date:10/9/2017)... ... October 09, 2017 , ... The Giving Tree Wellness Center ... the needs of consumers who are incorporating medical marijuana into their wellness and ... , As operators of two successful Valley dispensaries, The Giving Tree’s two founders, ...
Breaking Biology Technology: