Navigation Links
A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
Date:2/7/2013

tissue, in a compact environment where they touch their neighbours. When they are cultivated they stick to the bottom and grow until they come in contact with other cells thus forming a monolayer," adds the researcher.

Avoiding cell stress

According to Brags, the electroporation method used up to now is very aggressive. Firstly, an enzyme must be added to detach the cells adhered to the bottom of the culture plate. Then they must be transferred to a special cuvette with electrodes where the electrical discharge is applied. Lastly, the cells must then be returned to the plate. All this combined causes stress in the cells and only a fraction survives. This translates into significant losses.

"We have developed a small plate in the shape of a disk. In its lower face it contains a collection of electrodes that allow for high output electroporation. The devices are made to match the size of the plate containing the cultures, the most common being a centimetre in diameter."

Ramn Brags explains that the added value of the device is that it allows the electroporation in the recipient in which cell culture is already taking place without the need for them to be extracted. This usually occurs in Petri dishes or multiwell plates. The device incorporates microseparations that ensure that the disk is situated some 10 microns apart. Therefore, without touching or crushing the cells the discharge is performed under 20 volts and then removed.

"We initially thought of developing a device with microelectronic technology but we managed to do it with printed circuit technology, which is much cheaper. Each disk ends up costing less than one Euro per unit, which is very competitive compared to current devices that go from one Euro to 100 euros per unit," points out the researcher.

In addition, Brags points out that the low cost of the new devices means that laboratories can use them on a single use basis. This eliminates cultu
'/>"/>

Contact: SINC
info@agenciasinc.es
34-914-251-820
FECYT - Spanish Foundation for Science and Technology
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Spanish researcher releases a video showing a beetle from the inside
2. Spanish scientist participate in the most comprehensive study ever done on ice
3. Spanish consumers prefer national fish
4. Spanish researchers design biomarkers for the detection of dengue and West Nile virus
5. Breakthroughs in Chikungunya research from A*STAR spell new hope for better treatment and protection
6. Interventional radiology: Potential breakthrough to treat mens enlarged prostate
7. WanderID Launches Breakthrough ID Product for Children, Seniors
8. Nanotechnology breakthrough could dramatically improve medical tests
9. NTU researchers study little mighty creature for scientific breakthrough
10. Research breakthrough: High brain integration underlies winning performances
11. Breakthrough technology focuses in on disease traits of single cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
(Date:7/25/2014)... puberty is influenced by ,imprinted genes,a subset of genes whose ... This is the first evidence that imprinted genes can control ... study were published today in the journal Nature . ... a marker for the timing of puberty in females. ... girls, is an inherited trait, and is linked to breast ...
(Date:7/24/2014)... (July 24, 2014) Scientists at The New ... one step closer to creating a viable cell ... own cells. , For the first time, ... lines from skin samples of patients with primary ... accelerated protocol to induce these stem cells into ...
(Date:7/24/2014)... species for food or commercial food sale, has been ... decline of many species of primates and other mammals. ... half of the species being consumed are birds, particularly ... only the meat made available for sale but the ... hunters and brought to villages for consumption, we noted ...
Breaking Biology News(10 mins):Could age of first period influence development of diseases in older women? 2NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2
... around the ancient spruce woodlands of eastern North ... could respond to climate change? Matt Fitzpatrick of ... Appalachian Laboratory, along with researchers from the University ... received a three-year, $670,000 award from the National ...
... Uppsala University, Sweden, instil hope of efficient hydrogen production with ... scepticism based on previous research. The study, which is published ... view on the ability of green algae which is ... producing fuel from renewable energy sources to replace the fossil ...
... Monell Center molecular neurobiologist Peihua Jiang, PhD, is ... Investigators in Gustation, an annual award presented by the ... scientist who is a rising leader in the field ... the understanding of the structure and function of taste ...
Cached Biology News:Scientists look to ancient past to better predict how species may respond climate change 2Surprising findings on hydrogen production in green algae 2Peihua Jiang receives Ajinomoto Award for Young Investigators in Gustation from AChemS 2
(Date:7/24/2014)... Engineering at the University of Delaware, is known ... energy engineering, environmental sustainability and electronics. , His ... with a well-defined, crystalline structure. At the atomic ... that zeolites can separate molecules with size differences ... of a nanometer), making them useful to the ...
(Date:7/24/2014)... (PRWEB) July 24, 2014 Your ... advances in modeling lithium-ion battery storage capacity. , ... from a theoretical model created at Lawrence ... that predicts how carbon components will perform ... storage emphasizes the urgent need for higher-performance batteries. ...
(Date:7/24/2014)... 2014 2014 Deep Research Report ... a professional and in-depth research report on Potassium ... basic information, including its definition, classification, application, ... research covers the international market analysis, including China’s ... covering macroeconomic environment & economic situation analysis, the ...
(Date:7/24/2014)... The first part of the session ... in Human Studies” will cover comprehensive clinical support in ... With increasing R&D costs and declining industry success in ... the knowledge collected in early Phase I/IIa study data ... late stage development. , Next, the presenters will examine ...
Breaking Biology Technology:University of Delaware researcher describes new approach for creating organic zeolites 2Getting More Life out of Lithium-ion Batteries 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 2Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 3Potassium Sulphate Market & SHMP Industry 2014 (Global, China) Analysis Now Available at DeepResearchReports.com 4Best Practices for Optimizing Dose Escalation in First in Human Studies, New Webinar August 6th Hosted by Xtalks 2
... April 4, 2011 Reportlinker.com announces that ... in its catalogue: Antiviral ... http://www.reportlinker.com/p0203535/Antiviral-Therapeutics---Technologies-markets-and-companies.html ... of antiviral approaches including vaccines, pharmaceuticals and ...
... 2011 Reportlinker.com announces that a new market ... Nanobiotechnologies- applications, markets and companies ... Nanotechnology is the creation and utilization of materials, ... on the nanometer-length scale (a nanometer is one ...
... 4, 2011 GeneLink, Inc. (OTCBB: GNLK, "the ... reported financial results for the fiscal year ended December 31, ... Gross margin increased from $4,906,710 in 2009 to $4,997,367 in ... 2010 as compared to $8,561,455 in 2009; Operating losses ...
Cached Biology Technology:Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 2Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 3Reportlinker Adds Antiviral Therapeutics - Technologies, Markets and Companies 4Reportlinker Adds Nanobiotechnologies - Applications, Markets and Companies 2Reportlinker Adds Nanobiotechnologies - Applications, Markets and Companies 3GeneLink Reports 2010 Results 2
Goat polyclonal to Dysadherin ( Abpromise for all tested applications). entrezGeneID: 53827...
Ku80 Antibody Shipping Temperature: HOT Storage Temperature: -20C...
This antibody detects an ~25 kDa band, corresponding to the expected molecular mass of Rab3A, on immunoblots. The antibody specificity is confirmed in peptide inhibition immunoblotting studies....
Rabbit polyclonal to Shigella (HRP) ( Abpromise for all tested applications)....
Biology Products: