Navigation Links
A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
Date:2/7/2013

tissue, in a compact environment where they touch their neighbours. When they are cultivated they stick to the bottom and grow until they come in contact with other cells thus forming a monolayer," adds the researcher.

Avoiding cell stress

According to Brags, the electroporation method used up to now is very aggressive. Firstly, an enzyme must be added to detach the cells adhered to the bottom of the culture plate. Then they must be transferred to a special cuvette with electrodes where the electrical discharge is applied. Lastly, the cells must then be returned to the plate. All this combined causes stress in the cells and only a fraction survives. This translates into significant losses.

"We have developed a small plate in the shape of a disk. In its lower face it contains a collection of electrodes that allow for high output electroporation. The devices are made to match the size of the plate containing the cultures, the most common being a centimetre in diameter."

Ramn Brags explains that the added value of the device is that it allows the electroporation in the recipient in which cell culture is already taking place without the need for them to be extracted. This usually occurs in Petri dishes or multiwell plates. The device incorporates microseparations that ensure that the disk is situated some 10 microns apart. Therefore, without touching or crushing the cells the discharge is performed under 20 volts and then removed.

"We initially thought of developing a device with microelectronic technology but we managed to do it with printed circuit technology, which is much cheaper. Each disk ends up costing less than one Euro per unit, which is very competitive compared to current devices that go from one Euro to 100 euros per unit," points out the researcher.

In addition, Brags points out that the low cost of the new devices means that laboratories can use them on a single use basis. This eliminates cultu
'/>"/>

Contact: SINC
info@agenciasinc.es
34-914-251-820
FECYT - Spanish Foundation for Science and Technology
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Spanish researcher releases a video showing a beetle from the inside
2. Spanish scientist participate in the most comprehensive study ever done on ice
3. Spanish consumers prefer national fish
4. Spanish researchers design biomarkers for the detection of dengue and West Nile virus
5. Breakthroughs in Chikungunya research from A*STAR spell new hope for better treatment and protection
6. Interventional radiology: Potential breakthrough to treat mens enlarged prostate
7. WanderID Launches Breakthrough ID Product for Children, Seniors
8. Nanotechnology breakthrough could dramatically improve medical tests
9. NTU researchers study little mighty creature for scientific breakthrough
10. Research breakthrough: High brain integration underlies winning performances
11. Breakthrough technology focuses in on disease traits of single cells
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
(Date:12/17/2014)... 16, 2014 Valencell, a leader in performance ... technology to industry leaders such as Intel, Jabra, Atlas, ... biometric wearable products. These products will be showcased at ... Las Vegas . "Our partners ... and robust – with the clinical data to back ...
(Date:12/15/2014)... , Dec. 12, 2014 Research and ... the addition of the "Global Facial Recognition ... http://photos.prnewswire.com/prnh/20130307/600769 ... identification of individuals. Facial recognition system measures the ... nose, jaw edges, mouth, and the distance between ...
(Date:12/11/2014)... SALEM, N.C. , Dec. 10, 2014  That blood ... for quite a while. Hypertension – the medical term for ... in the early 1800s, and the inflatable cuff that,s used ... That doesn,t, however, mean there,s nothing new about hypertension, its ... some long-held beliefs about the condition and the best ways ...
Breaking Biology News(10 mins):Valencell PerformTek Biometrics Power the Most Accurate Wearables at CES 2015 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 2Global Facial Recognition Market 2015-2019: Key Vendors are 3M Cogent, Cognitec Systems, NEC and Safran Group 3Research points to need for new approaches to treatment of high blood pressure 2Research points to need for new approaches to treatment of high blood pressure 3Research points to need for new approaches to treatment of high blood pressure 4
... cost of saving a teenage life? When it comes ... there,s no price tag. Parents with good intentions, however, ... not protecting their teens from the ticking timebomb of ... premium-quality medical supplies, has formed an exclusive partnership with ...
... bottle or finger sucking may hamper a child,s speech ... In a study that took place in Patagonia, Chile, ... an increased risk of speech disorders in preschool children. ... certain word sounds and to simplify their pronunciation. ...
... the key to new ways to fight cancer and ... role in regulating circadian rhythm, says Liheng Shi, a ... Circadian rhythm is the roughly 24-hour cycle of physiological ... He and colleagues have had their research, currently focusing ...
Cached Biology News:MEDNET Launches MedSafe to Prevent Teenage Prescription Drug Abuse 2MEDNET Launches MedSafe to Prevent Teenage Prescription Drug Abuse 3Infant sucking habits may affect how baby talks 2Texas A&M researchers find new mechanism for circadian rhythm 2
(Date:12/17/2014)... Achim Noack has been named global vice president ... position, Noack will assist Jerry Stoller, CEO and president ... and supervise global marketing management and implementation of new ... and experience in the crop protection industry,ā€ says Jerry ... thinking will be a great asset for Stoller Group, ...
(Date:12/17/2014)... 2014 Gene synthesis and bioengineering ... entered into a technology access and services agreement ... DNA2.0ā€™s proprietary protein engineering technology, known as ā€œProteinGPS®ā€ ... are extremely excited that the global leader ADM ... proprietary bioengineering technology has now been validated through ...
(Date:12/17/2014)... 17, 2014 Tigercat Pharma, Inc. today ... evaluating the investigational oral NK-1 receptor antagonist VPD-737 ... chronic itch who are unresponsive or inadequately responsive ... steroids and antihistamines. The primary ... Scale (VAS) itch score from baseline, comparing drug ...
(Date:12/15/2014)... -- GlassesOff Inc. (OTCBB: GLSO) announced today the appointment of ... of the Company,s Board of Directors. Mr. ... CEO until its acquisition by Stanley Black ... as the inventor of the first Wi-Fi-based Active RFID ... RFID solutions focused on improving operational efficiency, safety and ...
Breaking Biology Technology:Achim Noack Joins Stoller Group as Global Vice President of Marketing and Administration 2ADM and DNA2.0 Enter Into Protein Engineering Technology Access and Service Agreement 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3
... developed by a University of Wisconsin-Madison Engineering professor could ... such devices as laptop computers and provide insights into ... This device will allow researchers to study for the ... on single electron transport in transistors. ...
... the first-ever Wisconsin Entrepreneurs Conference in Milwaukee last week, it ... what they were doing and what they planned to do. ... a plan to rid Lake Erie of the dreaded zebra ... quickly making it the scourge of the entire Great Lakes ...
... 29 Berbee Information Networks Corporation, a leading Midwest provider ... today that it has been selected by Microsoft as ... and Visual Studio .NET 2003 Launch. , , ... Express Center inMilwaukee, Wisconsin, Berbee will speak in depth ...
Cached Biology Technology:Cool Computers 2Cool Computers 3Entrepreneurial Spirit Infectious in Milwaukee 2Entrepreneurial Spirit Infectious in Milwaukee 3
assays for phosphatase, lipase, nucleoside triphosphatases (ATPase, GTPase etc). HTS and follow up studies for phosphatase inhibitors. Quantitation of phosphate content in protein, lipids, and DNA....
One-step, microplate or cuvet, colorimetric, detection range 0.1 mg/dL to 3 mg/dL. Procedure: 10 min....
Human DCIR/CLEC4A Phycoerythrin MAb (Clone 216110)...
Contains 100 positive controls for effective screening of HDAC inhibitors by fluorometric method....
Biology Products: