In a "relaxed" double-helical segment of DNA, the two strands twist around the helical axis once every 10.4-10.5 base pairs of sequence. Adding or subtracting twists, as some enzymes can do, imposes strain. If a DNA segment...
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... twisting it about the other strand, to relax DNA to a thermodynamic equilibrium. Once DNA is relaxed, topoisomerase reconnects broken strands. Structure ...
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... with the amount of relaxed DNA to get you going and ... Relaxed, modified pBR-322 1000 ug -High purity DNA gyrase holoenzyme 2000 units -Assay buffer ...
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... enzyme with the amount of relaxed DNA to get you going and ... Relaxed, modified pBR-322 500 ug -High purity DNA gyrase holoenzyme 1000 units -Assay buffer ...
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Relaxation of DNA-loops was analysed by the method of anomalous viscosity time ... The dose-responses for DNA-loop relaxation, induction of at least one residual ...
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All topoisomerases can relax supercoiled DNA, and DNA gyrase, present in ... the inter-conversion of relaxed and supercoiled DNA, is not readily monitored. ...
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... way to estimate the relaxation time of DNA in varying degrees of ... relaxation times of DNA were extracted ... The relaxation behavior of DNA confined in a ...
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Any deviation from this relaxed state increases the energy of the DNA molecule. ... Relaxed DNA is in the "B" configuration, with 10 base pairs per helical turn. ...
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DNA in its relaxed (ideal) state usually assumes the B configuration, a right ... Any deviation from this relaxed state increases the energy of the DNA molecule. ...
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DNA in its relaxed (ideal) state usually assumes the B configuration. In a relaxed double-helical segment of DNA, the two strands twist around the ...
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The active DNA inside a cell is not relaxed DNA, but instead ... DNA is said to be relaxed (FIGURE ... For the relaxed DNA shown in FIGURE 44), the ...
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The DNA relaxation function of TOP1 is essential for the fundamental biological ... The supercoiled and relaxed DNA bands are shown. ...
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overwound compared with the relaxed DNA. ... in shape between supercoiled and relaxed DNA molecules. ... relaxed intracellular DNA, which is usually achieved ...
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This loss of superhelical stress puts the plasmid into a "relaxed DNA" form. ... micrograph: This one is of relaxed DNA. Relaxed DNA (Bock lab) What is a ...
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... to Relaxed and Linear Forms in Nucleobond AX Plasmid DNA Preparations ... DNA using the Nucleobond AX alkaline lysis-based protocol, occasionally relaxed ...
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DNA relaxation assay was used to characterize the purified MtTOP. ... Relaxation of negatively supercoiled DNA to determine unit activity. ...
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